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nCounter Academy: SPRINT

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Welcome to training on the nCounter SPRINT platform!

The nCounter SPRINT Profiler has been designed to accelerate sample-to-data with a simple workflow and – minimal hands on time. This training will provide you with all the information you need to run the nCounter SPRINT System. Let’s get started!

Introduction
Start Here
Hybridization
Preparing a Run
Sample Processing
Maintenance
Troubleshooting
Need More Help?
Introduction

nCounter Technology Overview

Now that you are preparing to take on biomolecule profiling using the nCounter, it is necessary to get an overview of the assay and the functions of the instrumentation. For a quick orientation on the nCounter technology, check out this short 2-minute video:

nCounter Technology by NanoString

Start Here

Before Training on the nCounter SPRINT

Before beginning training on the SPRINT, it is necessary to gather all the reagents needed for the training. For any NanoString Assay on the nCounter Platform, please consult the appropriate hybridization manual for a list of required ancillary equipment required for the assay, such as a thermocycler and pipettes.

In addition to laboratory equipment, please confirm all assay reagents are present and stored at the correct temperature.

nCounter SPRINT Training Introduction (<1 minute)

For information on RNA preparation and sample quality control for the nCounter SPRINT, please consult the sample preparation manuals for RNA from FFPE tissue or fresh frozen materials.

Hybridization

Sample Hybridization for the SPRINT

For training on the SPRINT, we will be demonstrating how to perform the gene expression assay using the reagents supplied with the nCounter Training kit. This video will walk you through the steps of preparing the CodeSet hybridization reaction using the Customer Assay Evaluation (CAE) training kit. For a step-by-step protocol of the CodeSet hybridization process, please consult the CodeSet Hybridization Setup user manual (applicable for experiments utilizing nCounter Panels and nCounter Custom CodeSets).

nCounter SPRINT CodeSet Hybridization Setup (6 minutes)

Preparing a Run

The SPRINT Control Center

We will walk you through the steps to prepare the instrument with all your sample information before your run and discuss all the file types associated with the SPRINT profiler. Information found in the following videos is also described in depth in the SPRINT user manual.

nCounter SPRINT File Definitions (4 minutes)

nCounter SPRINT Control Center (5 minutes)

Sample Processing

Processing on the SPRINT Profiler

This section will demonstrate how to load samples into the SPRINT cartridge, initiate a run on the instrument, and download the data following a run. The quick start guide for loading samples on a SPRINT cartridge provides a detailed overview of how to prepare samples for the SPRINT instrument. This information will also be demonstrated in the video tutorial below. Further information for each step of this process can also be found in the SPRINT user manual.

Loading Samples onto the SPRINT Cartridge (7 minutes)

Initiating a run on the SPRINT Instrument (4.5 minutes)

Downloading Data from the SPRINT (2 minutes)

Maintenance

nCounter SPRINT Maintenance

In this section, you will find resources for routine maintenance for your nCounter SPRINT to ensure the longevity of your instrument. This information is also available in the SPRINT user manual. For any questions or for further information on instrument maintenance, please contact our technical support team.

Top 10 Tips and Tricks on the nCounter SPRINT

  1. Record the lot number of the reagents you use with each experiment. Many users will peel the labels from CodeSet tubes and cartridges and insert these directly into a lab notebook for easy reference.
  2. Connect your SPRINT to your local network to access the Web Application, which lets you remotely enter run information, check run status, download data, and more.
  3. Always warm your SPRINT cartridge to room temperature for at least 15 minutes before loading your samples.
  4. When loading your SPRINT cartridge, always add an air gap behind your sample. Simply depress your pipette plunger past the second stop to add an air bubble. It’s okay if the bubbles are slightly different sizes, the instrument will take care of the rest.
  5. Never force or apply pressure to the cartridge drawer, instead use the open and close commands on the touchscreen.
  6. When initializing a run, stay with your SPRINT for the first 5–10 minutes to ensure that it initiates without errors. In general, any errors that occur during this time will be recoverable.
  7. Used the wrong RLF for your scan? You can easily re-define an RLF by using the “Fix RLF” function in the Web Application.
  8. For faster response times when contacting Technical Support, attach your instrument log files to an email request to support@nanostring.com. Log files can be downloaded via Sprint GUI or Web Application.
  9. Always check your data for quality control immediately after each run, even if you are not ready to perform your complete data analysis at that time. Checking your data after each run is especially important if you are performing a large experiment over many months.
  10. For a lab with multiple users, you can enable usernames and passwords to keep your run information safe.

Exchanging SPRINT Buffers (9.5 minutes)

For further information on the SPRINT instrument, please consult our instrument knowledgebase or download the SPRINT FAQ.

Troubleshooting

nCounter SPRINT Troubleshooting

In this section, you will find resources that can be used for troubleshooting any issues with assays on the SPRINT. This information is also available in the SPRINT user manual. The quick guide on sending log files to the NanoString support team can be found here. For any questions or for further information on instrument maintenance, please contact our technical support team.

Downloading Log Files from the nCounter SPRINT (4 minutes)

For further information on the SPRINT instrument, please consult our instrument knowledgebase or download the SPRINT FAQ.

Need More Help?

How to Contact Support

Have data analysis questions or what to learn more about options to analyze your nCounter data? Make sure to check out the nCounter Academy Data Analysis section!