Dynamically Choose Housekeepers

Number of Housekeepers Auto-Select

"Auto-select" utilizes geNorm to identify the optimal number of housekeepers.

Dynamically Choose Housekeepers
Uses the geNorm algorithm (Vandesompele et al., Genome Biology, 2002) to identify the most stably expressed genes up to the number specified from among the annotated housekeepers and normalizes the data using these genes. This option is recommended for use with raw data. Selection of this option with normalized data will override normalization performed in the experiment wizard.

Threshold Low Count Data

Threshold Count Value Observation Frequency

Threshold Low Count Data
Removes genes from the analysis based on a specified threshold count value and observation frequency across all samples. Genes that fall below the threshold at a frequency greater than the specified observation frequency will be removed from the analysis.

Threshold count value: (min = 0, max = 100)

Observation frequency: (min = 0, max = 1)

Choose additional image types to create

Select optional additional image output format - .png files will be created automatically