The nCounter Analysis System utilizes a novel digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (< 1 copy per cell). The technology uses a molecular "barcodes" and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.
Each color-coded barcode is attached to a single target-specific probe corresponding to a gene of interest. Mixed together with controls, they form a multiplexed CodeSet.
- Multiplex hundreds of gene targets in a single reaction
- High sensitivity (< 1 copy per cell)
- Fully automated system
- Exceptionally easy-to-use
- No enzymes required to perform assay
- Analyze DNA, RNA, and protein simultaneously in a single reaction
- 10 invariant region controls included in every Codeset
Each color-coded barcode represents a single target molecule. Barcodes hybridize directly to your target molecules and can be individually counted without the need for amplification—providing sensitive digital data.
NanoString protein analysis leverages antibodies for detection of proteins of interest that are barcoded with unique synthetic DNA oligonucleotides. This enables protein detection where each DNA oligonucleotide is recognized by a unique Reporter probe that contains a fluorescent barcode.
- The Power of Single-Molecule Counting
By Joseph Beechem, SVP of Research & Development
- Accelerating Cancer Research with 3D Biology: Simultaneous Detection of DNA, RNA and Protein in FFPE Samples
By Gordon B. Mills, M.D., Ph.D. MD Anderson Cancer Center
For Research Use Only. Not for use in diagnostic procedures.