Biomarker Discovery with Morphological Context
Morphology driven high plex profiling on a single tissue section
- Highly multiplexed protein or RNA detection: up to 96 proteins and over 1000 RNA targets
- Minimum sample: protein or RNA analysis from a single FFPE section
- Morphological context: whole slide 4 color imaging to guide profiling
- Maintains sample integrity: samples can be reused
- Digital quantitation: up to 6 logs (base 10) dynamic range
- Tunable resolution: select from 600 micron regions of interest down to single-cell
- High throughput: up to 20 sections per day
Available through our Technology Access Program
Changing How Tissue Specimens are Analyzed
Historically, immunohistochemistry and immunofluorescence have been used to assess spatial heterogeneity of proteins and nucleic acids in tissue slices. However, these techniques are of limited utility due to restricted dynamic range, difficult quantitation, and limited multiplexing capability.
NanoString’s GeoMx™ Digital Spatial Profiler* combines standard immunofluorescence techniques with digital optical barcoding technology to perform highly multiplexed, spatially resolved profiling experiments. In a single reaction, the DSP technology performs whole slide imaging with up to four fluorescent stains to capture tissue morphology and select regions of interest for high plex profiling. The ability to perform tissue morphology guided profiling experiments increases the likelihood of capturing rare events often missed by typical grind and bind assays. The DSP chemistry enables spatially resolved high plex profiling of RNA and protein targets on just two serial sample sections. The ability to perform multianalyte analysis enables deep characterization of the sample.
What is Digital Spatial Profiling?
GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Profiling is based on nCounter® barcoding technology and enables spatially resolved, digital readout of up to 96 proteins or RNA targets in a multiplexed assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags. After hybridization of probes to slide-mounted formalin fixed paraffin-embedded (FFPE) tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are quantitated in a standard nCounter assay, and counts are mapped back to tissue location, yielding a spatially-resolved digital profile of analyte abundance.
The DSP Workflow
- Process: Apply high-plex oligo-labeled antibody cocktail + morphology markers
- View: Use visible wavelength low-plex imaging to establish tissue "geography." Select regions-of-interest (ROIs) for high-plex profiling
- Profile: UV-release oligo tags at selected ROIs
- Plate: Store released tags in microtiter plate, index, and hybridize to barcodes
- Digitally count up to 1 million data points per ROI
- Analyze the data with nSolver™Advanced Analysis Software
Many different applications can be supported on GeoMx Digital Spatial Profiler*. The content menu is continuously expanding. Two key applications explored during Technology Access include:
1. Tumor and tumor microenvironment profiling
2. Profiling of microglial populations surrounding plaque formation
*Commerically available 2019
For all available content, please contact us through our Technology Access Program.
- Highly multiplexed analysis of immune cell subsets in non-small cell lung cancer: validation of protein and RNA analysis by the NanoString Digital Spatial Profiling (DSP) platform
- Validation of novel high-plex protein spatial profiling quantitation based on NanoString's Digital Spatial Profiling (DSP) technology with quantitative fluorescence (QIF)
- Spatially resolved, multiplexed digital characterization of protein and mRNA distribution and abundance in formalin-fixed, paraffin-embedded (FFPE) tissue sections based on NanoString’s Digital Spatial Profiling (DSP) technology: applications to immuno-oncology (IO) and tumor heterogeneity
- Spatially resolved, multiplexed digital characterization of protein and mRNA abundance in FFPE tissue sections: application to immuno-oncology
- Spatially resolved, multiplexed digital characterization of protein and RNA expression in FFPE tissue sections
- Spatially resolved, multiplexed digital characterization of protein and RNA distribution and abundance in FFPE tissue sections
- A new approach for immuno-oncology biomarker discovery: high-plex, spatial protein profiling based on NanoString digital quantification
- Multidimensional spatial characterization of the tumor microenvironment in synchronous melanoma metastases yields insights into mixed responses to therapy
- (Neo-)adjuvant ipilimumab + nivolumab (IPI+NIVO) in palpable stage III melanoma: Updated data from the OpACIN trial and first immunological analyses
For Research Use Only. Not for use in diagnostic procedures.