nCounter® ChIP-String Assay

The nCounter ChIP-String Assay is a read-out method designed to measure dsDNA fragments enriched via user-defined chromatin immunoprecipitation protocols. Based on nCounter Technology, the assay reliably quantifies as few as 5,000 molecules of DNA without amplification. The assay is ideal for validating ChIP-Seq results and screening large sample sets against focused sets of loci involved in chromatin remodeling and gene expression. Additionally, streamline the screening of ChIP antibodies, library pre-sequencing, or optimize IP protocols. nCounter ChIP-String delivers the same level of performance, flexibility, and low hands-on time as other nCounter assays.

  • No library prep or amplification required
  • Accurate differentiation/quantification of enriched DNA and excellent correlation with ChIP-Seq results
  • Analyze up to 800 loci in 24 hours with 15 minutes of hands-on time

Description

Specifications

Maximum number of probes per CodeSet

800

Recommended size of target region submitted

> 300 bases

Recommended amount of starting material

~10ng unamplified ChIP DNA
~100ng of WGA amplified ChIP DNA or NGS library prepared DNA

Sample type supported

Genomic DNA prepared by ChIP

Hybridization reaction volume

30 μL

Synthetic spike titration correlation

> 0.95

Linear dynamic range

7 x 10⁵ total counts

nCounter Prep Station throughput

12 samples < 2.5 hours

nCounter Digital Analyzer throughput

12 samples/2.7 hours (up to 108 samples per day unattended running in continuous mode)

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For Research Use Only. Not for use in diagnostic procedures