The Fine Balance of Immunology
The immune system often plays a role in the onset and progression of different disorders such as autoimmunity, fibrosis, and cancer. Understanding which cell types, cytokines, pathways, and transcription factors are involved in the balance between immune activation and suppression can lead to better treatments for chronic inflammation and disease. In addition to traditional bulk expression and proteomic analysis, spatial profiling of the location of immune cells in tissue can yield a better understanding of disease pathogenesis, uncovering differences within individuals as well as novel biomarkers for stratification and treatment.
Challenges
We know it’s a challenge as an immunologist to piece together what underlies healthy versus exhausted or abnormal immune system function. Projects often involve many different research techniques, cell types, and biomolecules and you may be working with a variety of sample types. Even more of a challenge is understanding how different immune cells and biomolecules function and communicate in situ in the tissue in response to disease.
How much could you advance your understanding of the immune system if you had access to a multiplexed technology platform for transcriptomic and proteomic analysis of multiple sample types such as Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections, fresh frozen tissue, cell lysates, PBMCs and whole blood? Make an impact on human health faster with streamlined bulk and spatial analysis of RNA, protein, and immune cell types using the combined power of the nCounter® Analysis System and the GeoMx® Digital Spatial Profiler.
Featured Solutions
Choose from curated, multiplexed nCounter Gene Expression Panels, nCounter Vantage 3D™ Protein Assays, GeoMx DSP RNA Assays, and/or GeoMx Protein Assays to build your next experiment and discover answers to your immunology questions. Take advantage of embedded immune cell typing signatures in nCounter expression panels to quantify the relative abundance of 14 different immune cell types or run an nCounter panel downstream of FACs to profile specific immune cell populations.
Publications
Remodeling of the tumor microenvironment via disrupting Blimp1(+) effector Treg activity augments response to anti-PD-1 blockade.
BACKGROUND: Accumulation of Foxp3(+) regulatory T (Treg) cells in the tumor often represents an important mechanism for cancer immune evasion and a critical barrier to anti-tumor immunity and immunotherapy. Many tumor-infiltrating Treg cells display an activated phenotype and express the transcription factor Blimp1.
Gene expression of oxidative stress markers and lung function: A CARDIA lung study.
BACKGROUND: Circulating markers of oxidative stress have been associated with lower lung function. Our objective was to study the association of gene expression levels of oxidative stress pathway genes (ALOX12, ALOX15, ARG2, GSTT1, LPO, MPO, NDUFB3, PLA2G7, and SOD3) and lung function forced expiratory volume in one second (FEV1 ), forced vital capacity (FVC) in Coronary Artery Risk Development in Young Adults study.
Circadian dynamics of the teleost skin immune-microbiome interface.
Background: Circadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, however, is yet to be realised.
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