Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model

Andrey Elchaninov; Maria Nikitina; Polina Vishnyakova; Anastasia Lokhonina; Andrey Makarov; Gennady Sukhikh; Timur Fatkhudinov

doi:10.1016/j.biopha.2021.111516

Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model

Biomed Pharmacother. 2021 Jun:138:111516. doi: 10.1016/j.biopha.2021.111516. Epub 2021 Mar 23.

Authors

Andrey Elchaninov¹, Maria Nikitina², Polina Vishnyakova³, Anastasia Lokhonina³, Andrey Makarov⁴, Gennady Sukhikh⁵, Timur Fatkhudinov⁶

Affiliations

¹ Laboratory of Regenerative Medicine, FSBI National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow 117997, Russia; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow 117198, Russia. Electronic address: elchandrey@yandex.ru.
² Laboratory of Growth and Development, FSBSI Scientific Research Institute of Human Morphology, Moscow 117418, Russia.
³ Laboratory of Regenerative Medicine, FSBI National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow 117997, Russia; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow 117198, Russia.
⁴ Laboratory of Regenerative Medicine, FSBI National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow 117997, Russia; Histology Department, Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow 117997 Russia.
⁵ Laboratory of Regenerative Medicine, FSBI National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow 117997, Russia.
⁶ Laboratory of Growth and Development, FSBSI Scientific Research Institute of Human Morphology, Moscow 117418, Russia; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow 117198, Russia.

PMID: 33765583
DOI: 10.1016/j.biopha.2021.111516

Abstract

Macrophages are important regulators of liver repair. Participation of migratory monocytes/macrophages in regeneration of hepatic tissues after resection remains disputable. In mouse the resection promotes migration of Ly6C+CD11b+ monocytes/macrophages to the remnant liver accompanied by a reduction in its CD206 + macrophage content. Macrophage proliferation within the liver reaches maximum on day 3 after the surgery. Corresponding macro- and microtranscriptomic profiles of macrophages in regeneration liver cannot be unambiguously defined as pro- or anti-inflammatory. Their typical features include elevated expression of leukocyte chemoattractant factors, and many of the differentially expressed sequences are related to the control of cell growth and metabolic processes in the liver. These findings revealed essential roles of immigration of monocytes/macrophages and macrophages proliferation in maintenance of macrophage populations in the mouse liver during its recovery from a massive resection.

Keywords: Liver; Macrophage; MicroRNA; Monocyte; NanoString; Regeneration.

MeSH terms

Animals
Cells, Cultured
Disease Models, Animal*
Hepatectomy / methods*
Ki-67 Antigen / biosynthesis
Ki-67 Antigen / genetics
Ki-67 Antigen / immunology
Liver / immunology
Liver / metabolism*
Liver / surgery
Liver Regeneration / physiology*
Macrophages / immunology
Macrophages / metabolism
Male
Mice
Mice, Inbred BALB C
Monocytes / immunology
Monocytes / metabolism*
Transcriptome / physiology*

Substances

Ki-67 Antigen