nCounter® Vantage 3D™ Lung Tumor Assays

Profile variations in DNA, RNA fusions, and protein expression simultaneously from minimal material. By combining multi-analyte, multiplex, digital measurements on a single platform, lung cancer researchers can gather more informative data from the most precious samples.

  • Detect 104 actionable SNV and INDELS, 63 lung gene fusions, and up to 28 total and phospho-proteins
  • Low sample inputs; as little as 5 ng DNA, 50 ng RNA, and 250 ng protein or just 2 FFPE slides
  • Integrated SNV, Fusion, and protein measurements allow for simplified data analysis
  • Compatible with your sample types, including cell or tissue lysate and FFPE

Integrated profiling of lung tumor FFPE samples

The following mutations were identified in 16 FFPE lung samples profiled:

Simultaneous analysis of samples with specific genotype revealed activation of the ERK MAPK pathway at the phospho-protein level in samples with activating KRAS mutations, as would be predicted.

Lung Cancer Profiling with 3D Biology Technology

Vantage 3D DNA SNV Solid Tumor Panel

Complete lung tumor characterization with detection of key SNV and INDELS.

Feature

Specifications

Number of Targets

104 SNV and INDELS covering 25 genes

Input Material

5 ng genomic DNA

Sample Type(s)

FFPE, fresh frozen tissue, cell extracts, cell lysates

Species

Human

Time to Results

<24 hours

Data Analysis

nSolver™ Analysis software

Shipping and Storage Conditions

-80°C (Amplification Reagents at -20°C)


Vantage 3D Lung Fusion Panel

Highly multiplexed assay capable of simultaneously characterizing key fusion events and selected reference genes in a reaction.

Feature

Specifications

Number of Targets

63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes.

Input Material

50–100 ng Purified Total RNA (~5000–10,000 cells) 150–300 ng FFPE extracted RNA

Sample Type(s)

FFPE, fresh frozen tissue, cell extracts, cell lysates

Species

Human

Time to Results

<24 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 24 additional genes or additional fusions

Shipping and Storage Conditions

-80°C


Vantage 3D Protein Solid Tumor Assay for Lysate

Profile lung cancer protein targets, including targets associated with PI3K, MAPK, and EGFR signaling.

Feature

Specifications

Number of Targets

28 protein targets, 3 controls

Input Material

250 ng protein

Sample Type(s)

Cell or fresh/frozen tissue lysates

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional targets

Shipping and Storage Conditions

-80°C


Vantage 3D Protein Solid Tumor Assay for FFPE

FFPE-compatible protein profiling of key lung cancer pathways.

Feature

Specifications

Number of Targets

26 protein targets, 3 controls

Input Material

One 5 µM FFPE slide

Sample Type(s)

FFPE (slide mounted)

Species

Human

Time to Results

<72 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional targets

Shipping and Storage Conditions

-80°C

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.

Click here for publications on RNA Fusions analysis.

Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D DNA SNV Solid Tumor Panel

Contains CodeSet for 104 solid tumor actionable mutations over 25 genes. Includes Pre-Amplification Reagents.

Note: this product is currently available on MAX/FLEX pre-qualified with the SNV Qualification kit.

VDXC-HST-12

12 reactions

nCounter® Vantage 3D Lung Gene Fusion Panel

Includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes.

VRXC-LGF-12

12 reactions (min order requires 60 reactions)

nCounter® Vantage 3D Protein Solid Tumor Panel for Lysates (D)

Contains Antibody Mix for 28 solid tumor signaling protein targets. Protein TagSet reagent (ERCC) for protein target detection.

To be used as a stand-alone protein assay for in silico merging with SNV and Fusion data. Compatible with lysate samples.

VPODC-HSTL-12

12 reactions

nCounter® Vantage 3D Protein Solid Tumor Panel for FFPE (D)

Contains AbMix for 26 solid tumor signaling protein targets. Protein TagSet reagent (ERCC) for protein target detection. To be used as a stand-alone protein assay for in silico merging with SNV and Fusion data. Compatible with FFPE samples.

VPODC-HSTF-12

12 reactions

For Research Use Only. Not for use in diagnostic procedures.