nCounter® Vantage 3D™ Solid Tumor Assays

Profile changes in DNA, RNA, and protein expression in a single experiment. The Vantage 3D DNA:RNA:Protein Solid Tumor Assays are designed to provide a complete solid tumor profiling solution for both genotype and phenotype data.

  • Low sample inputs: as little as 5 ng DNA, 25 ng RNA, and 250 ng protein or just 2 FFPE slides
  • Comprehensive profiling of key biological targets in Solid Tumor research: 104 actionable SNV and INDELS, 192 RNA, and up to 28 total and phospho-proteins
  • Integrated DNA, RNA, and protein measurements eliminate the challenge of comparing data from different technologies
  • Compatible with lysate and FFPE samples

Download the Application Notes today: Vantage 3D Profiling in FFPE Melanoma SamplesTargeted Drug Therapy Response, and PDX Profiling.


Monitor targeted drug therapy response

Drug response to Vemurafenib (+/- Trametinib) was most pronounced in the homozygous BRAF V600E cell line yielding significant changes in gene and protein expression and intracellular phosphorylation state consistent with the mechanism of action of therapeutic kinase inhibitors.

 

Vantage 3D Profiling in FFPE Melanoma Samples

Targeted Drug Therapy Response

PDX Profiling

Vantage 3D DNA:RNA:Protein Solid Tumor Assay for Lysate

Feature

Specifications

Number of Targets

104 SNV and INDELS covering 25 genes, 192 RNA including 12 reference genes, 28 proteins

Input Material

5 ng genomic DNA, 25-300 ng RNA, 250 ng protein

Sample Type(s)

Cell lysates or fresh/frozen tissue lysates

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 24 additional RNA targets and 3 protein targets

Shipping and Storage Conditions

-80°C (DNA Amplification Reagents at -20°C)


Vantage 3D DNA:RNA:Protein Solid Tumor Assay for FFPE

Feature

Specifications

Number of Targets

104 SNV and INDELS covering 25 genes, 192 RNA including 12 reference genes, 26 proteins

Input Material

Two 5 µM FFPE slides

Sample Type(s)

FFPE

Species

Human

Time to Results

<72 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 24 additional RNA targets and 3 protein targets

Shipping and Storage Conditions

-80°C (DNA Amplification Reagents at -20°C, FFPE Sample Prep Kit at 4°C)

 

Vantage 3D RNA:Protein Solid Tumor Assay for Lysate

Feature

Specifications

Number of Targets

770 RNA, 28 Proteins

Input Material

1 µg protein

Sample Type(s)

Cell lysates or fresh/frozen tissue lysates

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional protein targets

Shipping and Storage Conditions

-80°C

 

Vantage 3D RNA:Protein Solid Tumor Assay for FFPE

Feature

Specifications

Number of Targets

770 RNA, 26 Protein

Input Material

2 5 µM FFPE slides

Sample Type(s)

FFPE

Species

Human

Time to Results

<72 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional protein targets

Shipping and Storage Conditions

-80°C

  • Product Bulletin (RNA:Protein, SNV)
  • Protein Data Normalization and Analysis
  • Probe Lists
    • nCounter® Vantage 3D DNA:RNA:Protein Solid Tumor Assay for Lysate
    • Includes 104 probes for the specific detection of SNV relevant to solid tumors, and 40 probes for the detection of reference sequence corresponding to each variant, 192 RNA probes (from the Vantage 3D MAPK-PI3K Pathways Panel), and 28 protein probes related to key cell signaling pathways in solid tumors, as well as 3 protein controls for background estimation and normalization.
    • nCounter® Vantage 3D DNA:RNA:Protein Solid Tumor Assay for FFPE
      Includes 104 probes for the specific detection of SNV relevant to solid tumors, and 40 probes for the detection of reference sequence corresponding to each variant, 192 RNA probes (from the Vantage 3D MAPK-PI3K Pathways Panel), and 26 protein probes related to key cell signaling pathways in solid tumors, as well as 3 protein controls for background estimation and normalization.
    • nCounter® Vantage 3D RNA:Protein Solid Tumor Assay for Lysate
      Includes 770 RNA probes (from the nCounter® PanCancer Pathways Panel) and 28 protein probes related to key cell signaling pathways in solid tumors, as well as 3 protein controls for background estimation and normalization.
    • nCounter® Vantage 3D RNA:Protein Solid Tumor Assay for FFPE
      Includes 770 RNA probes (from the nCounter® PanCancer Pathways Panel) and 26 protein probes related to key cell signaling pathways in solid tumors, as well as 3 protein controls for background estimation and normalization.

 

Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D DNA:RNA:Protein Solid Tumor for Lysates

Contains: CodeSet for 104 solid tumor actionable mutations over 25 genes. DNA Pre-Amplification Reagents. 192 gene expression CodeSet (Vantage 3D MAPK-PI3K Pathways). Antibody Mix for 28 solid tumor signaling protein targets. Protein plus reagent (no ERCC) for protein target detection. Compatible with lysate samples. Note: this product is currently available on MAX/FLEX pre-qualified with the SNV Qualification kit.

VDRPC-HSTL-12

12 reactions

nCounter® Vantage 3D DNA:RNA:Protein Solid Tumor for FFPE

Contains: CodeSet for 104 solid tumor actionable mutations over 25 genes. DNA Pre-Amplification Reagents. 192 gene expression CodeSet (Vantage 3D MAPK-PI3K Pathways). 26 solid tumor signaling protein targets. Protein plus reagent (no ERCC) for protein target detection. Protein FFPE Sample Prep Kit, which contains Buffer W and Buffer T. Compatible with FFPE samples.

VDRPC-SPKP-HSTF-12

12 reactions

nCounter® Vantage 3D RNA:Protein Solid Tumor for Lysates 770 gene expression CodeSet (PanCancer Pathways), Antibody Mix for 28 solid tumor signaling protein targets. Protein plus reagent (no ERCC) for protein target detection. Compatible with lysate samples. VRPC-HSTL-12 12 reactions
nCounter® Vantage 3D RNA:Protein Solid Tumor for FFPE 770 gene expression CodeSet (PanCancer Pathways), 26 solid tumor signaling protein targets. Protein plus reagent (no ERCC) for protein target detection. Protein FFPE Sample Prep Kit, which contains Buffer W and Buffer T. Compatible with FFPE samples. Compatible with FFPE samples. VRPC-SPKP-HSTF-12 12 reactions

For Research Use Only. Not for use in diagnostic procedures.