Measure key intracellular protein targets including cytokines, chemoattractants, and transcription factors for the earliest indications of cell-cell communication and immune differentiation. The panel enables precise, multiplex, digital detection of critical protein targets in your immunology research.
- 26-plex protein detection from as few as 20,000 cells
- Content designed to enable characterization of cell trafficking, inflammation, STAT signaling, cell differentiation, and immune effector functions
- Compatible with fresh and cryopreserved cell suspensions, including PMBC
- Simultaneous multi-analyte profiling with Vantage 3D RNA and DNA Assays
- Customize panel content with up to 5 proteins of your choice
Content Overview
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Immunologic Role |
Protein Targets |
|
Chemokines |
CCL5, CXCL5, CXCL8 |
|
Cytokines |
IL-1β, IL-2, IL-6, TGFβ, IL-13 |
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Transcription Factors |
Aiolos, BaF, FoxP3, GATA3, Helios, Ikaros, STAT1, STAT3, T-bet, Th-Pok, IRF4, Bcl-6 |
|
Additional Immune Function |
GM-CSF, IFNγ, Perforin, IL-15RA, ARG1, PTEN |
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Feature |
Specifications |
|
Number of Targets |
26 protein targets, 3 controls |
|
Input Material |
20,000 cultured cells (50,000 primary cells) |
|
Sample Type(s) |
Fresh or cryopreserved cell suspensions, including PBMC |
|
Species |
Human |
|
Time to Results |
<48 hours |
|
Data Analysis |
nSolver™ Analysis software |
|
Shipping and Storage Conditions |
-80°C (Universal Cell Capture Kit at 4°C) |
- White Paper
- Tech Note
- Probe lists
- nCounter® Vantage 3D Protein Immune Cell Signaling (D)
Includes 26 protein targets plus 3 controls for use as a stand-alone protein assay or for use with Vantage 3D DNA (contains ERCC controls) - nCounter® Vantage 3D Protein Immune Cell Signaling (R)
Includes 26 protein targets plus 3 controls for use with Vantage 3D RNA (+/-DNA) (does not contain ERCC controls)
- nCounter® Vantage 3D Protein Immune Cell Signaling (D)
- Data Normalization and Analysis
- Assay Validation
- Quick Start Guide
- Presented Poster
- PBMC Tips and Tricks
- nCounter Vantage 3D Protein Immune Cell Signaling Panel
- Overview (MAN-10045)
- Intracellular Protein Processing from Cell Suspensions (MAN-10067)
- Protein Only Hybridization Setup (MAN-10059)
Click here to view RNA:Protein Profiling.
Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.
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Product |
Description |
Catalog Number |
Unit Size |
|
nCounter® Vantage 3D Protein Immune Cell Signaling Panel for cell suspensions (D) + Universal Cell Capture Kit |
Contains Antibody Mix for 26 intracellular immunology protein targets. Protein TagSet (D) (ERCC) for protein target detection. Universal Cell Capture Kit—Intracellular Compatible, which contains universal cell capture beads, Buffer W, Buffer LH, Buffer PW, and Buffer FD. To be used as a stand-alone protein assay or with nCounter Vantage 3D DNA panels. Compatible with cell suspensions. |
VPODC-B2M-HISS-12 |
12 reactions |
|
nCounter® Vantage 3D Protein Immune Cell Signaling Panel for cell suspensions (R) + Universal Cell Capture Kit |
Contains Antibody Mix for 26 intracellular immunology protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Universal Cell Capture Kit—Intracellular Compatible, which contains universal cell capture beads, Buffer W, Buffer LH, Buffer PW, and Buffer FD. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Compatible with cell suspensions. |
VPRXC-B2M-HISS-12 |
12 reactions |
For Research Use Only. Not for use in diagnostic procedures.