Track protein expression of key cell surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status. The Immune Cell Profiling panel enables precise, digital detection of the most important cell surface targets in your immuno-oncology research.
- 30-plex protein detection from as few as 20,000 cells
- Comprehensive coverage of the Cancer Immunity Cycle
- Compatible with fresh and cryopreserved cell suspensions such as PMBC
- Multi-analyte analysis when combined with RNA assays
- Customize with up to 5 targets of your choice
Content Driven by the Cancer Immunity Cycle
Illustration used with permission and originally published in Immunity, Vol. 39, Daniel S. Chen and Ira Mellman, “Oncology Meets Immunology: The Cancer-Immunity Cycle”, pp 1-10, Copyright Elsevier, Inc. 2013.
|
Stage of Cancer Immunity Cycle |
Protein Targets |
|
(2) Antigen Presentation |
CD4, CD40, CD154 (CD40L) |
|
(3) Priming and Activation |
CD279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD25 (IL2R), CD56 (NCAM), CD357 (GITR), CD137 (OX40), CD27, CD28, CD127, CD137 (4-1BB) |
|
(4–5) Trafficking and Infiltration |
CD9 |
|
(6–7) Recognition and Killing of Cancer Cells |
D279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD272 (BTLA), HLA-DRA |
|
Immune Modulation |
CD279 (PD-1), CD278 (ICOS), CD158e1 (KIR3DL1), CD335 (NKp46), CD152 (CTLA-4), CD3 (CD3E), CD8 (CD8A), CD14, CD19, CD33, CD68, CD163 |
| Feature | Specifications |
|---|---|
| Number of Targets | 30 protein targets, 3 controls |
| Input Material | 20,000 cultured cells (50,000 primary cells) |
| Sample Type(s) | Fresh or cryopreserved cell suspensions, including PBMC |
| Species | Human |
| Time to Results | <48 hours |
| Data Analysis | nSolver™ Analysis software |
| Shipping and Storage Conditions | -80°C (Universal Cell Capture Kit at 4°C) |
- White Paper
- Tech Note
- Probe lists
- nCounter® Vantage 3D Protein Immune Cell Profiling (D)
Includes 30 protein targets plus 3 controls for use as a stand-alone protein assay (contains ERCC controls) - nCounter® Vantage 3D Protein Immune Cell Profiling (R)
Includes 30 protein targets plus 3 controls for use with RNA panels (does not contain ERCC controls)
- nCounter® Vantage 3D Protein Immune Cell Profiling (D)
- Data Normalization and Analysis
- Assay Validation
- Quick Start Guide
- Presented Poster
- PBMC Tips and Tricks
- nCounter Vantage 3D Protein Immune Cell Profiling Panel
- Overview (MAN-10068)
- Cell Surface Protein Processing from Cell Suspensions (MAN-10066)
- Protein Only Hybridization Setup (MAN-10059)
Click here to view RNA:Protein Profiling.
Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.
| Product | Description | Catalog Number | Unit Size |
|---|---|---|---|
| nCounter® Vantage 3D™ Protein Immune Cell Profiling Panel for cell suspensions (D) + Universal Cell Capture Kit | Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein TagSet (D) (ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, Buffer W, and Buffer LH. To be used as a stand-alone protein assay. Compatible with cell suspensions. | VPODC-B2M-HIPS-12 | 12 reactions |
| nCounter® Vantage 3D™ Protein Immune Cell Profiling Panel for cell suspensions (R) + Universal Cell Capture Kit | Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, buffer W, and buffer LH. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter® Vantage 3D RNA Panels. Compatible with cell suspensions. | VPRXC-B2M-HIPS-12 | 12 reactions |
For Research Use Only. Not for use in diagnostic procedures.