nCounter® Vantage 3D™ Solid Tumor Assays

Profile changes in RNA, and protein expression in a single experiment. The Vantage 3D RNA:Protein Solid Tumor Assays are designed to provide a complete solid tumor profiling solution for both genotype and phenotype data.

  • Low sample inputs: as little as 25 ng RNA, and 250 ng protein or just 2 FFPE slides
  • Comprehensive profiling of key biological targets in Solid Tumor research: 192 RNA, and up to 28 total and phospho-proteins
  • Integrated RNA, and protein measurements eliminate the challenge of comparing data from different technologies
  • Compatible with lysate and FFPE samples

Download the Application Notes today: Vantage 3D Profiling in FFPE Melanoma Samples, and PDX Profiling.


Monitor targeted drug therapy response

Drug response to Vemurafenib (+/- Trametinib) was most pronounced in the homozygous BRAF V600E cell line yielding significant changes in gene and protein expression and intracellular phosphorylation state consistent with the mechanism of action of therapeutic kinase inhibitors.

 

Vantage 3D Profiling in FFPE Melanoma Samples

PDX Profiling

Vantage 3D™ RNA:Protein Solid Tumor Assay for Lysate

Feature Specifications
Number of Targets 770 RNA, 28 Proteins
Input Material 1 µg protein
Sample Type(s) Cell lysates or fresh/frozen tissue lysates
Species Human
Time to Results <48 hours
Data Analysis nSolver™ Analysis software
Customize Up to 5 additional protein targets
Shipping and Storage Conditions -80°C

 

Vantage 3D™ RNA:Protein Solid Tumor Assay for FFPE

Feature Specifications
Number of Targets 770 RNA, 26 Protein
Input Material 2 5 µM FFPE slides
Sample Type(s) FFPE
Species Human
Time to Results <72 hours
Data Analysis nSolver™ Analysis software
Customize Up to 5 additional protein targets
Shipping and Storage Conditions -80°C

Vantage 3D™ RNA:Protein Solid Tumor Assay for Lysate

Assay Overview MAN-10033

 

Vantage 3D™ RNA:Protein Solid Tumor Assay For FFPE

Assay Overview MAN-10035

Click here to view how cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.

Presented Posters:

Product Description Catalog Number Unit Size
nCounter® Vantage 3D™ RNA:Protein Solid Tumor for Lysates 770 gene expression CodeSet (PanCancer Pathways), Antibody Mix for 28 solid tumor signaling protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Buffer WS. Compatible with lysate samples. VRPC-SPKP-HSTL-12 12 reactions
nCounter® Vantage 3D™ RNA:Protein Solid Tumor for FFPE 770 gene expression CodeSet (PanCancer Pathways), 26 solid tumor signaling protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Protein FFPE Sample Prep Kit, which contains Buffer W and Buffer T. Compatible with FFPE samples. Compatible with FFPE samples. VRPC-SPKP-HSTF-12 12 reactions

For Research Use Only. Not for use in diagnostic procedures.