Vantage 3D™ Immuno-Oncology Assays

Quantify protein and gene expression in one experiment and gain deeper insight into your immuno-oncology research. Designed with protein content matched to the gene expression 770-plex PanCancer Immune Profiling Panel for characterization of up to 800 total targets, these assays enable more data from less sample.

  • Detect 770 mRNA and up to 30 proteins from as few as 20,000 cells
  • Content designed to profile immune cell type and functional state
  • Compatible with PBMC and other primary cells
  • Simple integrated workflow and holistic data output
  • Easy data management: No bioinformatician required

Compatible with 3D Flow™ Analysis

Request 3D Flow Analysis Guidelines

Post-translational Regulation

NanoString data demonstrates that combined RNA and protein profiling reveals potential post-transcriptional regulation for a subset of targets and protein expression data that is highly correlated with gold standard flow cytometry.

 

data is highly correlated with gold standard flow cytometry

PBMC were stimulated with PMA and RNA and protein expression was profiled using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay (input of 50,000 PBMC). Additionally, a subset of protein targets were analyzed by flow cytometry and data from both platforms showed high correlation.

 

Characterizing Macrophage Differentiation and Polarization

Integrated analysis enables simultaneous quantitation of RNA and protein expression from a single sample and eliminates the need to compare data from different technologies with different outputs.

simultaneous quantitation of RNA and protein expression from a single sample

 

A THP-1 monocytic cell line was induced to differentiate into a macrophage phenotype with PMA. Cells were then polarized to an M1 or M2 phenotype by 24h culture using IFNγ + LPS, or IL4, respectively. Gene and protein expression was characterized at each stage of differentiation using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay.

 

Precise Cell Profiling

RNA:Protein signatures can accurately identify specific immune cell types, allowing you to uncover changes in a heterogeneous cell population in response to external stimuli, such as immunotherapeutic agents, for example.

Vantage 3D™ RNA:Protein Immune Cell Profiling Assay

Track RNA and protein expression of key surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status

Feature Specifications
Number of Targets 770 RNA, 30 Proteins
Input Material 20,000 cultured cells (50,000 primary cells)
Sample Type(s) Fresh or cryopreserved cell suspensions, including PBMC
Species Human
Time to Results <48 hours
Data Analysis nSolver™ Analysis software
Customize Up to 5 additional protein targets
Shipping and Storage Conditions -80°C (Universal Cell Capture Kit at 4°C)

Click here to view RNA:Protein Profiling.

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.

Product Description Catalog Number Unit Size
nCounter® Vantage 3D™ RNA:Protein Immune Cell Profiling Assay for cell suspensions + Universal Cell Capture Kit 770 gene expression CodeSet (PanCancer Immune Profiling), Antibody mix for 30 Extracellular immunology protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Universal Cell Capture Kit – Cell Surface Compatible, which contains universal cell capture beads, Buffer W and Buffer LH.

Compatible with cell suspensions.
VRPC-B2M-HIPS-12 12 reactions

For Research Use Only. Not for use in diagnostic procedures.