Quantify protein and gene expression in one experiment and gain deeper insight into your immuno-oncology research. Designed with protein content matched to the gene expression 770-plex PanCancer Immune Profiling Panel for characterization of up to 800 total targets, these assays enable more data from less sample.
- Detect 770 mRNA and up to 30 proteins from as few as 20,000 cells
- Content designed to profile immune cell type and functional state
- Compatible with PBMC and other primary cells
- Simple integrated workflow and holistic data output
- Easy data management: No bioinformatician required
Compatible with 3D Flow™ Analysis
Request 3D Flow Analysis Guidelines
Post-translational Regulation
NanoString data demonstrates that combined RNA and protein profiling reveals potential post-transcriptional regulation for a subset of targets and protein expression data that is highly correlated with gold standard flow cytometry.
PBMC were stimulated with PMA and RNA and protein expression was profiled using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay (input of 50,000 PBMC). Additionally, a subset of protein targets were analyzed by flow cytometry and data from both platforms showed high correlation.
Characterizing Macrophage Differentiation and Polarization
Integrated analysis enables simultaneous quantitation of RNA and protein expression from a single sample and eliminates the need to compare data from different technologies with different outputs.
A THP-1 monocytic cell line was induced to differentiate into a macrophage phenotype with PMA. Cells were then polarized to an M1 or M2 phenotype by 24h culture using IFNγ + LPS, or IL4, respectively. Gene and protein expression was characterized at each stage of differentiation using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay.
Precise Cell Profiling
RNA:Protein signatures can accurately identify specific immune cell types, allowing you to uncover changes in a heterogeneous cell population in response to external stimuli, such as immunotherapeutic agents, for example.
Vantage 3D™ RNA:Protein Immune Cell Profiling Assay
Track RNA and protein expression of key surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status
| Feature | Specifications |
|---|---|
| Number of Targets | 770 RNA, 30 Proteins |
| Input Material | 20,000 cultured cells (50,000 primary cells) |
| Sample Type(s) | Fresh or cryopreserved cell suspensions, including PBMC |
| Species | Human |
| Time to Results | <48 hours |
| Data Analysis | nSolver™ Analysis software |
| Customize | Up to 5 additional protein targets |
| Shipping and Storage Conditions | -80°C (Universal Cell Capture Kit at 4°C) |
- White Papers
- Probe lists
- nCounter® Vantage 3D RNA:Protein Immune Cell Profiling
Includes 770 RNA probes (from the PanCancer Immune Profiling Panel) and 30 extracellular protein probes related to the detection of immune cells, tumor-specific antigens, and the assessment of immunological response, as well as 3 protein controls for estimation of background and normalization
- nCounter® Vantage 3D RNA:Protein Immune Cell Profiling
- Quick Start Guides
- Protein Data Normalization and Analysis
- Assay Validation
- Presented Poster
- nCounter Vantage 3D RNA:Protein Immune Cell Profiling Panel
- Overview (MAN-10031)
- Cell Surface Protein Processing from Cell Suspensions (MAN-10066)
- RNA:Protein Hybridization Setup (MAN-10060)
- Low RNA Input Amplification Kit (Optional) (MAN-10046)
Click here to view RNA:Protein Profiling.
Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.
| Product | Description | Catalog Number | Unit Size |
|---|---|---|---|
| nCounter® Vantage 3D™ RNA:Protein Immune Cell Profiling Assay for cell suspensions + Universal Cell Capture Kit | 770 gene expression CodeSet (PanCancer Immune Profiling), Antibody mix for 30 Extracellular immunology protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Universal Cell Capture Kit – Cell Surface Compatible, which contains universal cell capture beads, Buffer W and Buffer LH. Compatible with cell suspensions. |
VRPC-B2M-HIPS-12 | 12 reactions |
For Research Use Only. Not for use in diagnostic procedures.