nCounter® Vantage 3D™ Heme Assays

Profile changes in RNA and protein expression in a single experiment. The Vantage 3D™ RNA:Protein Heme Assays are designed to provide a complete heme profiling solution for both transcriptomic and proteomic data.

  • Low sample inputs: as little as 25 ng RNA and 250 ng protein
  • Comprehensive profiling of key biological targets in hematology-oncology research: 192 RNA and up to 35 total and phospho-proteins
  • Integrated RNA and protein measurements eliminate the challenge of comparing data from different technologies
  • Compatible with lysate and FFPE samples

Multi-analyte representation of canonical pathways underlying hematologic malignacies.

Multi-analyte representation of canonical pathways underlying hematologic malignacies. Green = Vantage 3D™ RNA Heme, Orange = Vantage 3D™ Protein Heme

Vantage 3D™ RNA:Protein Heme Assay for Lysate

Feature Specifications
Number of Targets 192 RNA including 12 reference genes, and 34 proteins
Input Material 25-300 ng RNA, 250 ng protein
Sample Type(s) Cell lysates or fresh/frozen tissue lysates
Species Human
Time to Results <48 hours
Data Analysis nSolver™ Analysis software
Customization Up to 24 additional RNA targets and 5 protein targets
Shipping and Storage Conditions -80°C (Amplification Reagents at -20°C)

 

Vantage 3D™ RNA:Protein Heme Assay for FFPE

Feature Specifications
Number of Targets 192 RNA including 12 reference genes, and 35 proteins
Input Material Two 5 µM FFPE slides
Sample Type(s) FFPE
Species Human
Time to Results <72 hours
Data Analysis nSolver™ Analysis software
Customization Up to 24 additional RNA targets and 5 protein targets
Shipping and Storage Conditions -80°C (Amplification Reagents at -20°C)

 

Click here to view our publications.

Product Description Catalog Number Unit Size
nCounter Vantage 3D™ RNA:Protein Heme Assay for Lysate (Vantage 3D™ RNA Heme Panel and Vantage 3D™ Protein Heme Panel for Lysates (R))

Combined assay of Gene Expression CodeSet (Vantage 3D Heme) with Antibody Mix and Protein (R) reagent (with buffer WS, no ERCC) for protein target detection. Compatible with lysate samples.

VRXC-HEME-12 & VPRXC-HHML-12 12 reactions
nCounter Vantage 3D™ RNA:Protein Heme Assay for FFPE (Vantage 3D™ RNA Heme Panel and Vantage 3D™ Protein Heme Panel for FFPE (R)) Combined assay of Gene Expression CodeSet (Vantage 3D Heme) with Antibody Mix and Protein (R) reagent (with buffer W and buffer T, no ERCC) for protein target detection. Compatible with FFPE samples. VRXC-HEME-12 & VPRXC-HHMF-12 12 reactions

 

For Research Use Only. Not for use in diagnostic procedures.