Vantage 3D™ Immuno-Oncology Assays

Quantify protein and gene expression in one experiment and gain deeper insight into your immuno-oncology research. Designed with protein content matched to the gene expression 770-plex PanCancer Immune Profiling Panel for characterization of up to 800 total targets, these assays enable more data from less sample.

  • Detect 770 mRNA and up to 30 proteins from as few as 20,000 cells
  • Content designed to profile immune cell type and functional state
  • Compatible with PBMC and other primary cells
  • Simple integrated workflow and holistic data output
  • Easy data management: No bioinformatician required

Post-translational Regulation

NanoString data demonstrates that combined RNA and protein profiling reveals potential post-transcriptional regulation for a subset of targets and protein expression data that is highly correlated with gold standard flow cytometry.


PBMC were stimulated with PMA and RNA and protein expression was profiled using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay (input of 50,000 PBMC). Additionally, a subset of protein targets were analyzed by flow cytometry and data from both platforms showed high correlation.


Characterizing Macrophage Differentiation and Polarization

Integrated analysis enables simultaneous quantitation of RNA and protein expression from a single sample and eliminates the need to compare data from different technologies with different outputs.


A THP-1 monocytic cell line was induced to differentiate into a macrophage phenotype with PMA. Cells were then polarized to an M1 or M2 phenotype by 24h culture using IFNγ + LPS, or IL4, respectively. Gene and protein expression was characterized at each stage of differentiation using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay.


Precise Cell Profiling

RNA:Protein signatures can accurately identify specific immune cell types, allowing you to uncover changes in a heterogeneous cell population in response to external stimuli, such as immunotherapeutic agents, for example.


Vantage 3D RNA:Protein Immune Cell Profiling Assay

Track RNA and protein expression of key surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status

Feature

Specifications

Number of Targets

770 RNA, 30 Proteins

Input Material

20,000 cultured cells (50,000 primary cells)

Sample Type(s)

Fresh or cryopreserved cell suspensions, including PBMC

Species

°Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional protein targets

Shipping and Storage Conditions

-80°C (Universal Cell Capture Kit at 4°C)


Vantage 3D RNA:Protein Immune Cell Signaling Assay

Measure RNA and intracellular protein levels of cytokines, chemoattractants, and transcription factors for the earliest indications of cell-cell communication and immune differentiation.

Feature

Specifications

Number of Targets

770 RNA, 26 Proteins

Input Material

20,000 cultured cells (50,000 primary cells)

Sample Type(s)

Fresh or cryopreserved cell suspensions, including PBMC

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Customize

Up to 3 additional protein targets

Shipping and Storage Conditions

-80°C (Universal Cell Capture Kit at 4°C)

Click here to view RNA:Protein Profiling.

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.

Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D RNA:Protein Immune Cell Profiling Assay for cell suspensions + Universal Cell Capture Kit

770 gene expression CodeSet (PanCancer Immune Profiling), Antibody mix for 30 Extracellular immunology protein targets. Protein plus reagent (no ERCC) for protein target detection. Universal Cell Capture Kit – Cell Surface Compatible, which contains universal cell capture beads, Buffer W and Buffer LH.

Compatible with cell suspensions.

VRPC-B2M-HIPS-12

12 reactions

nCounter® Vantage 3D RNA:Protein Immune Cell Signaling Assay for cell suspensions + Universal Cell Capture Kit

770 gene expression CodeSet (PanCancer Immune), Antibody Mix for 26 Intracellular immunology protein targets. Protein plus reagent (no ERCC) for protein target detection. Universal Cell Capture Kit - Intracellular Compatible, which contains universal call capture beads, Buffer W, Buffer LH, Buffer PW, and Buffer FD. Compatible with cell suspensions.

VRPC-B2M-HISS-12

12 reactions

For Research Use Only. Not for use in diagnostic procedures.