nCounter® Vantage 3D™ Heme Assays

Profile changes in DNA, RNA, and protein expression in a single experiment. The Vantage 3D DNA:RNA:Protein Heme Assays are designed to provide a complete heme profiling solution for both genotype and phenotype data.

  • Low sample inputs: as little as 5 ng DNA, 25 ng RNA, and 250 ng protein or just 2 FFPE slides.
  • Comprehensive profiling of key biological targets in hematology-oncology research: >100 actionable SNV and INDELS*, 192 RNA, and >35 total and phospho-proteins*
  • Integrated DNA, RNA, and protein measurements eliminate the challenge of comparing data from different technologies
  • Compatible with lysate and FFPE samples

Heme-diagram-web.jpg

Multi-analyte representation of canonical pathways underlying hematologic malignacies. Blue = Vantage 3D DNA SNV Heme*, Green = Vantage 3D RNA Heme, Orange = Vantage 3D Protein Heme*

*The Vantage 3D DNA SNV Heme and Vantage 3D Protein Heme Panels are currently available for pre-sale only. Please contact your account representative for more information.

Vantage 3D DNA:RNA:Protein Heme Assay for Lysate

Feature Specifications
Number of Targets >100 SNV and INDELS covering >30 genes*, 192 RNA including 12 reference genes, and >35 proteins*
Input Material 5 ng genomic DNA, 25-300 ng RNA, 250 ng protein
Sample Type(s) Cell lysates or fresh/frozen tissue lysates
Species Human
Time to Results <48 hours
Data Analysis nSolver™ Analysis software
Customization Up to 24 additional RNA targets and 5 protein targets
Shipping and Storage Conditions -80°C (Amplification Reagents at -20°C)

 

Vantage 3D DNA:RNA:Protein Heme Assay for FFPE

Feature Specifications
Number of Targets >100 SNV and INDELS covering >30 genes*, 192 RNA including 12 reference genes, and >35 proteins*
Input Material Two 5 µM FFPE slides
Sample Type(s) FFPE
Species Human
Time to Results <72 hours
Data Analysis nSolver™ Analysis software
Customization Up to 24 additional RNA targets and 5 protein targets
Shipping and Storage Conditions -80°C (Amplification Reagents at -20°C)

 

*The Vantage 3D DNA SNV Heme and Vantage 3D Protein Heme Panels are currently available for pre-sale. Please contact us for more information.

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Product Description Catalog Number Unit Size
nCounter Vantage 3D DNA:RNA:Protein Heme Assay for Lysate 

Contains: CodeSet for SNV detection and DNA Pre-Amplification Reagents. 192 gene expression CodeSet (Vantage 3D Heme). Antibody Mix and Protein (R) reagent (no ERCC) for protein target detection. Buffer WS for protein sample antibody incubation. Compatible with lysate samples.

Note: this product is currently available on MAX/FLEX pre-qualified with the SNV Qualification kit.
VDRPC-HHML-12 12 reactions
nCounter Vantage 3D DNA:RNA:Protein Heme Assay for FFPE Contains: CodeSet for SNV detection and DNA Pre-Amplification Reagents. 192 gene expression CodeSet 

(Vantage 3D Heme). Antibody Mix and Protein (R) reagent (no ERCC) for protein target detection. Buffers required for protein sample preparation. Compatible with FFPE samples.

Note: this product is currently available on MAX/FLEX pre-qualified with the SNV Qualification kit.
VDRPC-HHMF-12 12 reactions

 

What I Need to Get Started

Additional materials required for sample preparation and analysis can be found in catalogue number specific protocol overviews. See “User Manuals” for further information.

 

Consumables Needed:

For nCounter® MAX/FLEX- Master Kit

For nCounter® SPRINT- SPRINT reagent pack and cartridge

nSolver™ Analysis Software

For Research Use Only. Not for use in diagnostic procedures.