nCounter® Vantage 3D™ Protein Solid Tumor Panel for Lysate

Perform multiplex analysis of phospho- and total proteins associated with key solid tumor signaling pathways, including MAPK, PI3K, and EGFR. The panel is designed to provide maximum pathway information from minimal cell and tissue lysates.

  • 28-plex protein expression analysis from just 250 ng protein
  • Content curated to profile key pathways in solid tumor biology
  • Compatible with cell and tissue lysates
  • 3D-enabled for multi-analyte analysis with other Vantage 3D RNA and DNA Assays
  • Customize with up to 3 additional targets

Curated Total and Phospho-Protein Content

Protein Targets

Driver Gene

MAPK

PI3K

Ras

Cell Cycle

4E-BP1 &

Phospho-4E-BP1 (Thr37/46)

-

-

+

-

-

EGF Receptor &

Phospho-EGF Receptor (Tyr1068)

+

+

+

+

-

GSK-3β &

Phospho-GSK-3β (Ser9)

-

-

+

-

+

HER2/ErbB2

+

+

+

+

-

Ki-67

-

-

-

-

+

Met

+

-

+

+

-

ERK &

Phospho-ERK (Thr202/Tyr204)

-

+

+

+

-

p53

+

+

+

-

+

Akt &

Phospho-Akt (Ser473)

+

+

+

+

+

Keratin

-

+

-

-

-

Phospho-AMPKα (Thr172)

-

-

+

-

-

Phospho-Chk1 (Ser345)

-

-

-

-

-

Phospho-c-Raf (Ser259)

-

+

+

+

-

Phospho-Histone H3 (Ser10)

-

-

-

-

+

Phospho-MEK1/2 (Ser217/221)

+

+

+

+

-

Phospho-p70 S6 Kinase (Thr389)

-

-

+

-

-

Phospho-PDK1 (Ser241)

-

-

+

-

-

Phospho-PRAS40 (Thr246)

-

-

+

-

-

Progesterone Receptor

+

-

-

-

-

S6 Ribosomal Protein &

Phospho-S6 Ribosomal Protein (Ser235/236)

-

-

+

-

-

Tuberin/TSC2 &

Phospho-Tuberin/TSC2 (Thr1462)

-

-

+

-

-



Feature

Specifications

Number of Targets

28 protein targets, 3 controls

Input Material

250 ng protein

Sample Type(s)

Cell or fresh/frozen tissue lysates

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Shipping and Storage Conditions

-80°C

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.


Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D Protein Solid Tumor Panel for Lysates (D)

Contains Antibody Mix for 28 solid tumor signaling protein targets. Protein TagSet reagent (ERCC) for protein target detection. To be used as a stand-alone protein assay or with nCounter Vantage® 3D DNA (when available). Compatible with lysate samples.

VPODC-HSTL-12

12 reactions

nCounter® Vantage 3D Protein Solid Tumor Panel for Lysates (R)

Contains AbMix for 28 solid tumor signaling protein targets. Protein plus reagent (no ERCC) for protein target detection. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter Vantage® 3D RNA Panels. Compatible with lysate samples.

VPRXC-HSTL-12

12 reactions

For Research Use Only. Not for use in diagnostic procedures.