nCounter® Vantage 3D™ Protein Immune Cell Signaling Panel

Measure key intracellular protein targets including cytokines, chemoattractants, and transcription factors for the earliest indications of cell-cell communication and immune differentiation. The panel enables precise, multiplex, digital detection of critical protein targets in your immunology research.

  • 26-plex protein detection from as few as 20,000 cells
  • Content designed to enable characterization of cell trafficking, inflammation, STAT signaling, cell differentiation, and immune effector functions
  • Compatible with fresh and cryopreserved cell suspensions, including PMBC
  • Simultaneous multi-analyte profiling with Vantage 3D RNA and DNA Assays
  • Customize panel content with up to 3 proteins of your choice

Content Overview

Immunologic Role

Protein Targets

Chemokines

CCL5, CXCL5, CXCL8

Cytokines

IL-1β, IL-2, IL-6, TGFβ, IL-13

Transcription Factors

Aiolos, BaF, FoxP3, GATA3, Helios, Ikaros, STAT1, STAT3, T-bet, Th-Pok, IRF4, Bcl-6

Additional Immune Function

GM-CSF, IFNγ, Perforin, IL-15RA, ARG1, PTEN



Feature

Specifications

Number of Targets

26 protein targets, 3 controls

Input Material

20,000 cultured cells (50,000 primary cells)

Sample Type(s)

Fresh or cryopreserved cell suspensions, including PBMC

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Shipping and Storage Conditions

-80°C (Universal Cell Capture Kit at 4°C)

Click here to view RNA:Protein Profiling.

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.


Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D Protein Immune Cell Signaling Panel for cell suspensions (D) + Universal Cell Capture Kit

Contains Antibody Mix for 26 intracellular immunology protein targets. Protein tagset reagent (ERCC) for protein target detection. Universal Cell Capture Kit—Intracellular Compatible, which contains universal cell capture beads, Buffer W, Buffer LH, Buffer PW, and Buffer FD. To be used as a stand-alone protein assay or with nCounter Vantage 3D DNA panels. Compatible with cell suspensions.

VPODC-B2M-HISS-12

12 reactions

nCounter® Vantage 3D Protein Immune Cell Signaling Panel for cell suspensions (R) + Universal Cell Capture Kit

Contains Antibody Mix for 26 intracellular immunology protein targets. Protein plus reagent (no ERCC) for protein target detection. Universal Cell Capture Kit—Intracellular Compatible, which contains universal cell capture beads, Buffer W, Buffer LH, Buffer PW, and Buffer FD. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Compatible with cell suspensions.

VPRXC-B2M-HISS-12

12 reactions

PBMC Tips & Tricks
Performing the Plate Flick
Cell Counting

For Research Use Only. Not for use in diagnostic procedures.