nCounter® Vantage 3D™ Protein Immune Cell Profiling Panel

Track protein expression of key cell surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status. The Immune Cell Profiling panel enables precise, digital detection of the most important cell surface targets in your immuno-oncology research.

  • 30-plex protein detection from as few as 20,000 cells
  • Comprehensive coverage of the Cancer Immunity Cycle
  • Compatible with fresh and cryopreserved cell suspensions such as PMBC
  • Multi-analyte analysis when combined with other Vantage 3D DNA and RNA assays
  • Customize with up to 3 targets of your choice

Content Driven by the Cancer Immunity Cycle

Illustration used with permission and originally published in Immunity, Vol. 39, Daniel S. Chen and Ira Mellman, “Oncology Meets Immunology: The Cancer-Immunity Cycle”, pp 1-10, Copyright Elsevier, Inc. 2013.


Stage of Cancer Immunity Cycle

Protein Targets

(2) Antigen Presentation

CD4, CD40, CD154 (CD40L)

(3) Priming and Activation

CD279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD25 (IL2R), CD56 (NCAM), CD357 (GITR), CD137 (OX40), CD27, CD28, CD127, CD137 (4-1BB)

(4–5) Trafficking and Infiltration

CD9

(6–7) Recognition and Killing of Cancer Cells

D279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD272 (BTLA), HLA-DRA

Immune Modulation

CD279 (PD-1), CD278 (ICOS), CD158e1 (KIR3DL1), CD335 (NKp46), CD152 (CTLA-4), CD3 (CD3E), CD8 (CD8A), CD14, CD19, CD33, CD68, CD163



Feature

Specifications

Number of Targets

30 protein targets, 3 controls

Input Material

20,000 cultured cells (50,000 primary cells)

Sample Type(s)

Fresh or cryopreserved cell suspensions, including PBMC

Species

Human

Time to Results

<48 hours

Data Analysis

nSolver™ Analysis software

Shipping and Storage Conditions

-80°C (Universal Cell Capture Kit at 4°C)


Click here to view RNA:Protein Profiling.

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.


Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D Protein Immune Cell Profiling Panel for cell suspensions (D) + Universal Cell Capture Kit

Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein TagSet reagent (ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, Buffer W, and Buffer LH. To be used as a stand-alone protein assay or with nCounter® Vantage 3D DNA panels. Compatible with cell suspensions.

VPODC-B2M-HIPS-12

12 reactions

nCounter® Vantage 3D Protein Immune Cell Profiling Panel for cell suspensions (R) + Universal Cell Capture Kit

Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein plus reagent (no ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, buffer W, and buffer LH. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter® Vantage 3D RNA Panels. Compatible with cell suspensions.

VPRXC-B2M-HIPS-12

12 reactions


PBMC Tips & Tricks
Performing the Plate Flick
Cell Counting

For Research Use Only. Not for use in diagnostic procedures.