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nCounter®
PanCancer IO 360™ Panel

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Helping Your Research

The nCounter PanCancer IO 360™ Panel and Data Analysis Service is designed specifically for immuno-oncology and translational researchers who are studying the impact of immune evasion in the tumor microenvironment and developing potentially prognostic, predictive or mechanism of action signatures for immunotherapy. This panel aids your research by providing potentially clinically actionable genes and signatures so you can better understand the mechanisms of immune evasion in the tumor and potentially predict response to immune-targeted and other therapies. This complete service combines the knowledge of incorporating essential genes and signatures with known or potential clinical relevance from the tumor, microenvironment, and immune response.

Inspired by systems biology approaches to cancer research, NanoString’s 360 Series Panel Collection gives you a 360° view of gene expression by combining carefully-curated content involved in the biology of the tumor, microenvironment, and the immune response into a single holistic assay. Each panel contains the 18-gene Tumor Inflammation Signature (TIS) that measures a peripherally-suppressed, adaptive immune response and has been shown to correlate with response to checkpoint inhibitors.

Biology and signatures

How it Works

The PanCancer IO 360 Gene Expression Panel is a unique 770 gene expression panel that combines vital components involved in the complex interplay between the tumor, microenvironment, and immune response in cancer, allowing for a multifaceted characterization of disease biology and interrogation of mechanisms of immune evasion. This powerful panel incorporates 48 potentially predictive Research Use Only (RUO) biological signatures including the 18-gene Tumor Inflammation Signature , and provides:

01:

Complete Coverage:

Unique 360-degree view of gene expression for tumor, microenvironment, and immune response. This holistic view enables research on the complex interactions that impact immune response and effectiveness of immunotherapies.

02:

Highly Curated Content:

Content developed by experts in industry and related to common combination immunotherapy drug targets.

03:

Rapid Insights:

An easy to interpret analytics report, available through the add-on Data Analysis Service for analysis of human IO 360 panel, provides information about distribution of TIS and other pre-defined IO signatures with the samples. Correlates signatures to clinical variables such as response, survival, mutation status, etc.

04:

Characterize disease biology:

Interrogate mechanisms of immune evasion and potentially predict response to immune-targeted and other therapies

Panel Selection Tool

Find the gene expression panel for your research with easy to use panel pro

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Product Information

Specifications
Signatures/ Pathways
Tumor Inflammation Signature
Product Comparison
Specifications
Signatures/ Pathways

Tumor-Microenvironment-Immune Response

NanoString synthesizes biological knowledge and large gene expression datasets together to train signatures of biological processes. For a given process, we use literature searches and expert knowledge to derive lists of candidate genes. For the PanCancer IO 360 panel, we evaluated the co-expression of candidate genes in data from The Cancer Genome Atlas (TCGA), discarding genes whose co-expression patterns are incompatible with measuring their putative biological process. This approach safeguards the interpretability of our signatures: we only report signatures whose genes show evidence for measuring the desired biology. Finally, we further exploit co-expression patterns to obtain optimal weights for each signature gene.

13 Biological Pathways and Processes

20 internal reference genes include overlapping genes from Hallmarks of Cancer PanCancer Collection for cross-panel comparisons.

Tumor Inflammation Signature

The Tumor Inflammation Signature includes 18 functional genes known to be associated with response to PD-1/PD-L1 inhibitors pathway blockade.

Includes 4 Areas of Immune Biology: IFN-ү-responsive genes related to antigen presentation, chemokine expression, cytotoxic activity, and adaptive immune resistance genes.

The tumor inflammation gene expression signature highlights the complex biology of the host immune microenvironment.

IO 360 Data Analysis Report 

Data analysis report for the human* nCounter PanCancer IO360™ Gene Expression Panel

  • Unique 360 view of gene expression for the tumor, microenvironment and immune response
  • Interactive reports prepared by NanoString expert scientists
  • 48 signatures including TIS, 14 signatures measuring immune cell populations and 34 novel signatures measuring important tumor and immune activities
  • Tumor Inflammation Score (TIS) provided for each sample to determine “hot” and “cold” tumors
  • Analysis includes sample signature score in relation to immune response
  • All data undergoes QC and normalization

Report only available for analysis of human panel data – report not available for mouse

Product Comparison

360 Series Product Comparison

Fully-annotated gene lists in Excel format are available for each of the 360 Panels. The table below compares the biology coverage of the 360 Panels across the tumor, microenvironment, and the immune response to that of the PanCancer Panels Collection.

Related Resources

See All Resources
Product Bulletin PanCancer IO 360 Panel- Product Bulletin
Flyer PanCancer IO 360 Biological Signatures – Flyer
Report IO 360 Demo Data Analysis Report
Report IO 360 Time Series Demo Analysis Report
Webinar Accelerating Immuno-Oncology Research with the PanCancer IO 360™ Gene Expression Panel, Advanced Data Analysis Service, and Signature Development

Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma

Synovial sarcoma (SyS) is an aggressive neoplasm driven by the SS18-SSX fusion, and is characterized by low T cell infiltration. Here, we studied the cancer-immune interplay in SyS using an integrative approach that combines single-cell RNA sequencing (scRNA-seq), spatial profiling and genetic and pharmacological perturbations.

Stepwise Reversal of Immune Dysregulation Due to STAT1 Gain-of-Function Mutation Following Ruxolitinib Bridge Therapy and Transplantation.

PURPOSE: Patients with heterozygous gain-of-function (GOF) mutations in STAT1 frequently exhibit chronic mucocutaneous candidiasis (CMC), immunodeficiency and autoimmune manifestations. Several treatment options including targeted therapies and hematopoietic stem cell transplantation (HSCT) are available for STAT1 GOF patients but modalities and outcomes are not well established.

Tumour inflammation signature and expression of S100A12 and HLA class I improve survival in HPV-negative hypopharyngeal cancer.

Hypopharyngeal squamous cell carcinoma (HPSCC) has a very poor prognosis. Local surgery may increase survival, but is often avoided due to significant post-op co-morbidities.

Skysphere

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