nCounter® miRNA Expression Panels
Helping Your Research
Conventional technologies for small RNA profiling like RNAseq can be costly, onerous, and require complex data analysis. In addition, they require RNA purification and multiple enzymatic steps that can be challenging for biofluid samples like serum and plasma. When developing reliable and robust miRNA signatures for different diseases, you need a robust and reliable platform that works well with multiple sample types.
nCounter miRNA Expression Panels utilize NanoString’s amplification-free technology to do expression profiling by direct quantification of individual RNA molecules. The assay detects miRNAs without the use of reverse transcription or amplification by using molecular barcodes. Therefore, it is easier and faster to validate miRNA biomarkers as compared to RNASeq or PCR-based platforms and the results are highly reproducible. With nCounter miRNA Expression Panels, you can:
- Reliably detect and quantitate the most biologically relevant human, mouse, or rat miRNAs directly from FFPE, blood, or biofluids
- Skip laborious library prep and process your samples with less than one hour of hands-on time
- Experience unparalleled reproducibility and specificity with a dynamic range of six logs
- Receive publication-ready figures within 24 hours with robust, off-the-shelf data analysis solutions
How It Works
nCounter miRNA Expression panels are designed to provide a sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levels of expression. The assay can be run on total RNA isolated from any source, including formalin-fixed paraffin embedded (FFPE) tissue sections.
Off-the-shelf assays are available for probing hundreds of miRNAs from human, mouse, and rat
Create a custom a la carte miRNA assay
Pick 20-50 miRNAs of your choice from the list of miRNAs included in the human, mouse, and rat miRNA panels
Custom design options
Available for human, mouse, and rat for the simultaneous analysis of mRNA and miRNA (miRGE Assays)
Panel Selection Tool
Find the gene expression panel for your research with easy to use panel proFind Your Panel
The spatial landscape of lung pathology during COVID-19 progression.
Recent studies have provided insights into the pathology and immune response to coronavirus disease 2019 (COVID-19)1–8. However, thorough interrogation of the interplay between infected cells and the immune system at sites of infection is lacking.
Spatial mapping of SARS-CoV-2 and H1N1 Lung Injury Identifies Differential Transcriptional Signatures.
Severe SARS-CoV-2 infection often leads to development of acute respiratory distress syndrome (ARDS), with profound pulmonary patho-histological changes post-mortem. It is not clear if ARDS from SARS-CoV-2 is similar to that observed in Influenza H1N1, another common viral cause of lung injury.
In-silico performance, validation, and modeling of the Nanostring Banff Human Organ transplant gene panel using archival data from human kidney transplants
RNA gene expression of renal transplantation biopsies is commonly used to identify the immunological patterns of graft rejection. Mostly done with microarrays, seminal findings defined the patterns of gene sets associated with rejection and non-rejection kidney allograft diagnoses.