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The Challenges of Reverse Transcription (RT)

For years, scientists have relied on platforms that use reverse transcriptase to convert RNA to cDNA in order to perform gene expression studies. However, the cDNA conversion and amplification steps can introduce variability into the data. Additionally, other steps in the workflow like sample prep and data analysis can introduce variability. The scientific community is now recognizing these challenges, and publications on the limitations of RT-based platforms are emerging across the qPCR, microarray and NGS spaces.

Workflow challenges versus nCounter benefits

The Benefits of Counting RNA Directly

What if you could bypass a cDNA conversion step and count RNA molecules directly? The nCounter® platform offers direct RNA detection for robust, reproducible performance and unbiased transcript quantitation.Interested in learning more? Your journey begins here

Predictive Value of Circulating miRNAs in Lymph Node Metastasis for Colon Cancer.

(1) Background: Lymph node (LN) status is an indubitable prognostic factor for survival among colon cancer patients. MicroRNAs (miRNAs) have been implicated in the development and progression of many cancers and are potential biomarkers for cancer diagnosis and prognosis.

Enrichment and Correlation Analysis of Serum miRNAs in Comorbidity Between Arnold-Chiari and Tourette Syndrome Contribute to Clarify Their Molecular Bases.

Due to its rarity, coupled to a multifactorial and very heterogeneous nature, the molecular etiology of Arnold-Chiari (AC) syndrome remains almost totally unknown. Its relationship with other neuropsychiatric disorders such as Tourette syndrome (TS) is also undetermined.

Fhit induces the reciprocal suppressions between Lin28/Let-7 and miR-17/92miR.

Objective: Fhit gene is known as a genome “caretaker” and frequently inactivated by deletion or hypermethylation on the promoter in several cancers. In spite of several lines of evidence, the exact mechanism underlying Fhit-induced biology is relatively less studied.

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