nCounter® miRNA Expression Panels

Identify and validate miRNA biomarkers faster and more reproducibly than with RNASeq or qPCR-based platforms. Analyze hundreds of biologically-relevant miRNAs from FFPE, cell lysates, exosomes, or biofluids in 24 hrs with a simple protocol that does not rely on RT or amplification.

  • Comprehensive, pre-built panels for human, mouse, and rat miRNAs
  • Skip the laborious library prep with less than one hour of hands-on time setup
  • Generate publication ready figures with robust, off-the-shelf data analysis solutions
  • Experience unparalleled reproducibility and specificity over a six-fold log10 dynamic range
  • Custom design option for the simultaneous analysis of mRNA and miRNA (miRGE Assays) 

Feature Specifications
Level of Multiplexing 827 human miRNAs, 577 mouse miRNAs, 423 rat miRNAs
Input Material 100 ng total RNA
Time to Results <24 hours
Sample Type(s) serum, plasma, FFPE, fresh frozen tissue, cell extracts, cell lysates
Species human, mouse, and rat
Data Analysis nSolver™ Analysis Software
Limit of Detection 0.5 fM spike-in control (~1 copy per cell); 90% of the time
Fold-change sensitivity > 1.5 fold (> 5 copies per cell)
> 2 fold change (> 1 copy per cell)
Spike Correlation R² ≥ 0.95
Linear Dynamic Range 7 x 10⁵ total counts
Controls See above
Shipping Conditions -80°C
Control Type Number Description Use
Positive Controls 6 Probes that recognize synthetic mRNA targets included in the CodeSet at specified concentrations (targets do not require ligation). Positive controls used by the QC metrics in nSolver to confirm linear response to input amounts, and confirm that low input signal is above background.
Negative Controls 8 Probes that recognize synthetic mRNA targets not included in the CodeSet (targets do not require ligation). Negative controls used by the QC metrics in nSolver to determine background signal independent of ligation success. This can then be used to set threshold for defining expression of miRNA.
Ligation Positive Controls 3 Probes that recognize synthetic miRNA targets included in the Sample Preparation Kit (Sample Preparation Kit includes targets, bridges, and miRtags). Ligation positive controls monitor ligation efficiency, independent of the miRNAs in the sample.
Ligation Negative Controls 3 Probes that recognize synthetic miRNA targets not included in the Sample Preparation Kit (no miRNA target). Ligation negative controls monitor non-specific ligation.
mRNA Reference Controls 5 Probes that recognize endogenous mRNA targets commonly expressed in tissues. Measurement of mRNA can be used to determine if there is cellular contamination in cell free samples. When miRNA is extracted from cells, even if ligation fails, the mRNA will be seen. It is not recommended that these mRNA be used as reference genes to normalize samples.
Spike-in Controls 5 Probes the recognize exogenous miRNA targets not included in the Sample Preparation Kit to monitor RNA isolation/ purification steps upstream of the assay (optional, added by user if desired). If user adds the appropriate small molecules to samples, they can be used to monitor steps of sample processing prior to the NanoString protocol.

Click here to view publication relative to Regulatory Small RNA

Product Description Catalog Number Unit Size
Human v3 miRNA Assay 827 miRNAs, 5 mRNAs, and 25 internal reference controls. CSO-MIR3-12 12 Assays
Mouse v1.5 miRNA Assay 577 miRNAs plus 33 murine-associated viral miRNAs, 4 mRNA probes, and 23 internal reference controls. CSO-MMIR15-12 12 Assays
Rat v1.5 miRNA Assay 423 miRNAs, 4 mRNAs, and 25 internal reference controls. CSO-RMIR15-12 12 Assays
À la carte miRNA Custom miRNA assay for Human, Mouse, or Rat made from 20-50 miRNAs taken from the list of miRNAs found in the Human, Mouse, or Rat assay. GXA-MICS-192 192 Assays
GXA-MICS-384 384 Assays
GXA-MICS-576 576 Assays
miRNA Assay Sample Preparation
miRNA Assay Protocol
microRNAs as bile-based biomarkers
Profiling miRNA Biomarkers in Cells, FFPE, and Clinical Biofluid Samples

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.