miRNA Assays

 

Discover miRNA biomarkers faster and more reproducibly with highly multiplexed, direct digital detection and quantification in a single assay.  nCounter miRNA Assays include off-the-shelf miRNA Panels for expression analysis of up to 800 human, mouse and rat samples and miRGE Assays for simultaneous measurement of user- defined miRNA and mRNA targets within the same sample.

nCounter miRNA Assays are compatible with a variety of sample types including FFPE, cell lysates, exosomes and biofluids and can be used for different areas of research such as cell signaling and cancer biology, neuroscience, stem cell research, autophagy, infectious disease, and immunology.

Choose Your miRNA Assay

  • miRNA Panels - Comprehensive, pre-built panels for human, mouse, and rat miRNAs
  • miRGE Assays - Custom designs for simultaneous analysis of mRNA and miRNA

 

"nCounter® microRNA panels have allowed us to comprehensively screen human and rat tissue for microRNA profiles in our disease setting of interest. This has allowed us to select candidate microRNA signatures of interest for expanded validation and further interrogation."

— Rachel Crossland, Ph.D., Newcastle University

NanoString’s FFPE-compatible chemistry allows for tens of thousands of data points to be generated per day in FFPE sample types that are important in translational research.

Christmann R. et al. (2016) performed biomarker analysis on tissue samples from patients with SSc-ILD to find miR-155 in the progression of lung fibrosis in systemic sclerosis using the nCounter® Human (V2) miRNA Expression Assay CodeSet.

Luciano C. et al. (2013) found interesting integrated miRNA and mRNA signatures associated with survival in triple-negative breast cancer using the nCounter® miRGE™ Assay (simultaneous analysis of miRNA and mRNA in the same sample).

The discovery of relatively stable, extracellular miRNAs in serum and plasma has generated huge interest as non-invasive biomarkers for a variety of diseases.

Permuth-Wey J. et al. (2015) discovered novel miRNA biomarkers in plasma samples for patients with intraductal papillary mucinous neoplasms of the pancreas using the nCounter® Human v3 miRNA Expression Assay .

Stylli S.S. et al. (2016) profiled miRNA in cerebrospinal fluid in patients with aneurysmal subarachnoid hemorrhage using the nCounter® Human v3 miRNA Expression Panel.

NanoString profiling using nCounter® Human v3 miRNA Expression Panel indicates differential serum expression levels of circulating miRNAs in healthy controls and children with mild or severe symptoms of EV71. Read more in Elevated expression of circulating miR876-5p is a specific response to severe EV71 infections by Wang R. et al. (2016).

Exosomal miRNAs play an important role in disease progression and can stimulate angiogenesis and facilitate metastasis in cancers 1.

 

Topol A. et al. (2016) used the nCounter® Human v3 miRNA Expression Assay to study dysregulation of miRNA-9 in a subset of schizophrenia patent-derived neural progenitor cells.

Rodosthenis S. et al. (2016) performed a pilot study of older individuals by studying ambient particulate matter and miRNA in extracellular vesicles using nCounter® Human (V2) miRNA Expression Assay CodeSet.

1 http://www.sciencedirect.com/science/article/pii/S167202291500011X

miRNAs have been identified as potential cancer biomarkers due to their presence and stability in many body fluids including urine.

Armstrong D.A. et al. (2015) profiled miRNA from matched tumor and biofluids in bladder cancer using the nCounter® Human v3 miRNA Expression Assay.

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For Research Use Only. Not for use in diagnostic procedures.