Simultaneously detect subsets of mRNA and miRNA in total RNA for biomarker discovery. The nCounter® miRGE assay is uniquely designed to provide a single-tube, sensitive, reproducible, and highly multiplexed method.
- Choose custom 5–30 miRNAs and 100–200 mRNAs for simultaneous analysis
- Available for human, mouse, and rat
- Compatible with total RNA from serum, plasma, FFPE, tissue, cells, and exosomes
- No reverse transcription, no amplification, and fewer pipetting steps means less chance for errors and bias in results
|Level of Multiplexing||100-200 mRNAs
|Recommended amount of starting material||100 ng of purified total RNA|
|Sample types supported||Purified total RNA|
|Sample prep reaction volume||10 μL|
|Hybridization reaction volume||35 μL|
|Limit of Detection: miRNA||> 2.5 copies per cell copy per cell|
|Limit of Detection: mRNA||1 copy per cell|
|Fold-change sensitivity||> 2-fold change|
|Hybridization Spike Correlation||R² ≥ 0.95|
|Linear Dynamic Range||2 x 10⁶ total counts|
|nCounter® Prep Station throughput||12 samples < 2.5 hours|
|nCounter® Digital Analyzer throughput||12 samples/4 hours (up to 72 samples per day) unmattended running in continuous mode)|
|Controls||6 positive hybridization controls
8 negative hybridization controls
2 positive ligation controls
|Unit Size||48, 96, 192 assays|
|Product||Description||Catalog Number||Unit Size|
|miRGE Assays||Custom assay measuring miRNAs and mRNAs.
Base price is the minimum configuration, 5 miRNAs and 100 mRNAs.
Additional mRNA and miRNA are added to the base price.
For Research Use Only. Not for use in diagnostic procedures.