GeoMx® Protein Assays
Helping Your Research
High-plex Spatial Proteomic Analysis
GeoMx Protein Assays enable quantitative, spatial analysis of 96+ proteins from a single FFPE or Fresh Frozen slide, vastly expanding the number of markers you can profile from a single tissue section compared to traditional immunohistochemical methods. All assays are validated for use in multiplex so you can focus on the biology of interest without wasting time troubleshooting assay performance.
How it Works
The assay relies upon antibodies coupled to photocleavable oligonucleotide tags designed for readout with nCounter® (≤96-plex) or NGS (over 100-plex) platforms. After hybridization of antibodies to slide-mounted tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are counted in a standard nCounter assay or sequenced using NGS, and counts are mapped back to tissue location yielding a spatially resolved digital profile of protein abundance.
All GeoMx® Protein Assays undergo extensive validation to ensure high quality GeoMx DSP data. Read more about GeoMx Antibody Validation in this white paper.
Product Information
Immuno-Oncology and Immunology
The Immune Cell Profiling Core contains 18 targets for broad cell profiling of human samples (17 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.
Neuroscience
The Neural Cell Profiling Core contains 20 targets designed for broad cell profiling of human samples (19 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.
Designed to profile 10’s-100’s of protein targets simultaneously with spatial resolution using NGS platforms and pipelines. Run the 4-plex GeoMx Protein Core for NGS, which includes necessary controls for GeoMx DSP experiments, with any selection of 10-plex modules.
*Panel offerings subject to change prior to commercial launch.
GeoMx Protein Assays can be customized with up to 10 antibodies through NanoString’s Protein Barcoding Service, or with up to 5 antibodies from Abcam. Learn more about our partnership with Abcam and choose from over 6,000 IHC-validated antibodies to customize a GeoMx protein assay directly from Abcam.
Related Resources
Publications
Models that combine transcriptomic with spatial protein information exceed the predictive value for either single modality.
Immunotherapy has reshaped the field of cancer therapeutics but the population that benefits are small in many tumor types, warranting a companion diagnostic test. While immunohistochemistry (IHC) for programmed death-ligand 1 (PD-L1) or mismatch repair (MMR) and polymerase chain reaction (PCR) for microsatellite instability (MSI) are the only approved companion diagnostics others are under consideration.
Cutaneous Melanoma Classification: The Importance of High-Throughput Genomic Technologies.
Cutaneous melanoma is an aggressive tumor responsible for 90% of mortality related to skin cancer. In the recent years, the discovery of driving mutations in melanoma has led to better treatment approaches.
Multiplexed digital spatial profiling of invasive breast tumors from Black and White women.
The NanoString GeoMx Digital Spatial Profiling (DSP) is a new multiplexed platform that quantifies the abundance of tumor- and immune-related proteins in a spatially resolved manner. We performed DSP for the simultaneous assessment of 52 analytes within spatially resolved tissue compartments defined by pan-cytokeratin expression.
Support Documents
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