GeoMx® Protein Assays
Helping Your Research
High-plex Spatial Proteomic Analysis
GeoMx Protein Assays enable quantitative, spatial analysis of 96+ proteins from a single FFPE or Fresh Frozen slide, vastly expanding the number of markers you can profile from a single tissue section compared to traditional immunohistochemical methods. All assays are validated for use in multiplex so you can focus on the biology of interest without wasting time troubleshooting assay performance.
How it Works
The assay relies upon antibodies coupled to photocleavable oligonucleotide tags designed for readout with nCounter® (≤96-plex) or NGS (over 100-plex) platforms. After hybridization of antibodies to slide-mounted tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are counted in a standard nCounter assay or sequenced using NGS, and counts are mapped back to tissue location yielding a spatially resolved digital profile of protein abundance.
All GeoMx® Protein Assays undergo extensive validation to ensure high quality GeoMx DSP data. Read more about GeoMx Antibody Validation in this white paper.
Product Information
Immuno-Oncology and Immunology
The Immune Cell Profiling Core contains 18 targets for broad cell profiling of human samples (17 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.
Neuroscience
The Neural Cell Profiling Core contains 20 targets designed for broad cell profiling of human samples (19 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.
Designed to profile 10’s-100’s of protein targets simultaneously with spatial resolution using NGS platforms and pipelines. Run the 4-plex GeoMx Protein Core for NGS, which includes necessary controls for GeoMx DSP experiments, with any selection of 10-plex modules.
*Panel offerings subject to change prior to commercial launch.
GeoMx Protein Assays can be customized with up to 10 antibodies through NanoString’s Protein Barcoding Service, or with up to 5 antibodies from Abcam. Learn more about our partnership with Abcam and choose from over 6,000 IHC-validated antibodies to customize a GeoMx protein assay directly from Abcam.
Related Resources
Publications
The spatial landscape of lung pathology during COVID-19 progression.
Recent studies have provided insights into the pathology and immune response to coronavirus disease 2019 (COVID-19)1–8. However, thorough interrogation of the interplay between infected cells and the immune system at sites of infection is lacking.
GeoMx RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue.
RNA in situ hybridization (ISH) is a widely used technique for the localization of mRNA in tissues. Limitations to traditional ISH include the number of targets that can be analyzed concurrently and the ability for many of these assays to be used in formalin-fixed, paraffin-embedded tissues (FFPE).
Spatial mapping of SARS-CoV-2 and H1N1 Lung Injury Identifies Differential Transcriptional Signatures.
Severe SARS-CoV-2 infection often leads to development of acute respiratory distress syndrome (ARDS), with profound pulmonary patho-histological changes post-mortem. It is not clear if ARDS from SARS-CoV-2 is similar to that observed in Influenza H1N1, another common viral cause of lung injury.
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