GeoMx™ Digital Spatial Profiler

Introducing...

GeoMxTM Digital Spatial Profiler
Your GPS for Biology

DSP_nanoString-9642_sm.jpgMorphological context and high-plex protein or RNA expression data – now possible from just one FFPE slide.

  • Quantify up to 96 proteins and over 1000 RNA targets with spatial context
  • Process 10-20 slides /day
  • Preserve precious samples with non-destructive processing

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GeoMx™ Digital Spatial Profiler
What’s been the biggest breakthrough during development of the Digital Spatial Profiling technology?
The fusion of molecular biology & pathology with Digital Spatial Profiling technology

 

For Research Use Only. Not for use in diagnostic procedures.

What is Digital Spatial Profiling?

GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Profiling is based on the nCounter® barcoding technology that enables spatially resolved, digital characterization of proteins or mRNA in a highly multiplexed (up to 1,000-plex) assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags. After hybridization of probes to slide-mounted formalin fixed paraffin-embedded (FFPE) tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are quantitated in a standard nCounter assay, and counts are mapped back to tissue location, yielding a spatially-resolved digital profile of analyte abundance.

The DSP Workflow

  1. Process: Apply high-plex oligo-labeled antibody cocktail + morphology markers
  2. View: Use visible wavelength low-plex imaging to establish tissue "geography." Select regions-of-interest (ROIs) for high-plex profiling
  3. Profile: UV-release oligo tags at selected ROIs
  4. Plate: Store released tags in microtiter plate, index, and hybridize to barcodes
  5. Digitally count up to 1 million data points per ROI
  6. Analyze the data with nSolver™Advanced Analysis Software

 

Publications:

Toki MI, Merritt CR, Wong PF, et al. High-plex predictive marker discovery for melanoma immunotherapy treated patients using Digital Spatial Profiling. CCR 2019; epub.

Amaria RN, Reddy SM, Tawbi HA, et al. Neoadjuvant immune checkpoint blockade in high-risk resectable melanoma. Nat Med 2018; 24(11): 1649-54.

Blank CU, Roseman EA, Fanchi LF, et al. Neoadjuvant versus adjuvant ipilimumab plus nivolumab in macroscopic stage III melanoma. Nat Med 2018; 24: 1655-1661.

Jérémie Decalf, Matthew L. Albert, James Ziai, New tools for pathology: a user’s review of a highly multiplexed method for in situ analysis of protein and RNA expression in tissue. J. Pathol 2018; epub.

Poster Presentations: