GeoMx® Digital Spatial Profiler
Your GPS for Biology
Morphological context and high-plex protein or RNA expression data – now possible from just one FFPE slide.
- Quantify up to 96 proteins and over 1000 RNA targets with spatial context
- Process 10-20 slides /day with readout on the nCounter® System or with NGS
- Preserve precious samples with non-destructive processing
What is Digital Spatial Profiling?
GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Profiling is based on the nCounter® barcoding technology that enables spatially resolved, digital characterization of proteins or mRNA in a highly multiplexed (up to 1,000-plex) assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags. After hybridization of probes to slide-mounted formalin fixed paraffin-embedded (FFPE) tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are quantitated in a standard nCounter assay, and counts are mapped back to tissue location, yielding a spatially-resolved digital profile of analyte abundance.
The DSP Workflow
- Process: Apply high-plex oligo-labeled antibody cocktail + morphology markers
- View: Use visible wavelength low-plex imaging to establish tissue "geography." Select regions-of-interest (ROIs) for high-plex profiling
- Profile: UV-release oligo tags at selected ROIs
- Plate: Store released tags in microtiter plate, index, and hybridize to barcodes
- Digitally count up to 1 million data points per ROI
- Analyze the data with nSolver™Advanced Analysis Software
GeoMx DSP Scan Checklist (MAN-10106-01)
GeoMx DSP Experimental Design Guideline (MAN-10108-01)
GeoMx DSP Example Data Analysis (MAN-10109-01)
Related Product Pages
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- Precise morphological mapping of high plex neurodegenerative and neuroinflammation protein targets in human FFPE brain tissue with the GeoMx™ Digital Spatial Profiler
- Comprehensive spatial RNA profiling of tumor, microenvironment and immune response pathways with the NanoString GeoMx™ Digital Spatial Profiler
- Multiple modalities of NanoString GeoMx™ Digital Spatial Profiler allow for spatially-resolved, multiplexed quantification of protein and mRNA distribution and abundance
- Integrating bulk and spatial profiling technologies for the discovery of RNA and protein biomarkers in muscle invasive bladder cancer
- Analysis of the immune microenvironment to advance breast cancer risk prediction and prevention
- In situ RNA expression profiling of 1600+ immuno-oncology targets in FFPE tissue using NanoString GeoMx™Digital Spatial Profiler
- High density of CD68+HLA-DR- macrophages in the stroma of primary melanoma correlates with an unfavorable immune microenvironment as assessed by Digital Spatial Profiling
- Spatially-resolved, high-plex digital profiling enables characterization of complex immune biology of the colorectal cancer tumor microenvironment
For Research Use Only. Not for use in diagnostic procedures.