nCounter® Gene Fusion Panels (For Laboratory Use)

nCounter® Gene Fusion Panels provide a highly-multiplexed assay capable of simultaneously characterizing key fusion events and probes for imbalanced gene expression in a single tube. The gene fusion panels utilize nCounter® Elements™ Reagents.  Combined with direct digital counting on the nCounter® Analysis System and our patented probe design, the detection of multiple fusion genes is highly sensitive, quantitative and easy.

  • Save time and sample material with multiplexed gene fusion detection in a single tube
  • Detect rare fusions with highly specific probes designed using patented Junction Sequence technology
  • Ideal for use with challenging sample types including FFPE tissue 
  • Developed For Laboratory Use using nCounter® Elements™ Reagents

Feature

Specifications

Input Material 50-110 ng Purified Total RNA (~5000-10,000 cells)
150-300 ng FFPE-extracted RNA
Hands on Time ~15 min
Time to Results Approximately 24 hours
Sample Type FFPE, fresh frozen tissue, cell extracts, cell lysates, blood lysates or blood fractions
Customizable Features Add up to 24 additional genes or additional fusions with the nCounter TagSet Extension product
Data Analysis nSolver™ Analysis Software (For Research Use Only)
Required Reagents

Panel CodeSet and Master Kit (nCounter MAX or FLEX Instruments)

Panel CodeSet, Cartridge and Reagent Pack (nCounter SPRINT Profiler)

The nCounter Lung Fusion Panel includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes.

Specific Lung Gene Fusion Probes detect the following gene fusion families:

ALK

RET

ROS

NTRK1

EML4-ALK

CCDC6-RET

CD74-ROS1

CD74-NTRK1

HIP1-ALK

KIF5B-RET

EZR-ROS1

MPRIP-NTRK1

KIF5B-ALK

GOPC-ROS1

TFG-ALK

LRIG3-ROS1

TPR-ALK

SDC4-ROS1


SLC34A2-ROS1


TPM3-ROS1

Lung Imbalance probes detect gene expression imbalance in the following genes:

ALK

RET

ROS

8 probes

8 probes

8 probes


The nCounter Leukemia Fusion Panel includes 42 probes in total: 27 for specific fusion detection, 12 leukemia genes, and 3 internal reference genes.

Leukemia Gene Fusion Probes detect the following gene fusion families:

CML

ALL

AML

AML/APL

BCR-ABL

E2A-PBX1

AML-ETO

PML-RARA


MLL-AF4

CBFB-MYH11



TEL-AML1

DEK-NUP214




RPN1-EVI1


Leukemia Gene Expression Probes:

Genes for AML, CML, and ALL

BAALC

ERG

FLT3

MECOM

MLLT11

MN1

NRAS

PRAME

RB1

SOCS2

TP53

WT1


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Product

Description

Catalog Number

Unit Size

nCounter Leukemia Gene Fusion Panel CodeSet Only

For Laboratory Use (Ex-US only). Includes 42 probes: 27 for specific fusion detection, 12 leukemia biomarkers, and 3 internal reference genes. CodeSet Only—does not include Master Kits. Price includes commercial use fee.

XT-CSO-XLKFU1-12

12 reactions

nCounter Lung Gene Fusion Panel CodeSet Only

For Laboratory Use (Ex-US only). Includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes. CodeSet Only—does not include Master Kits. Price includes commercial use fee.

XT-CSO-LGFU1-12

12 reactions