nCounter® Vantage 3D Gene Fusion Panels

Multiplexed analysis of gene fusion events associated with Leukemia and Lung cancer.

Combined with direct digital counting on the nCounter system and Junction Sequence probe design, the detection of fusion genes is highly sensitive, quantitative, and easy.

  • Save time and sample material with multiplexed gene fusion detection in a single tube
  • Detect rare fusions with highly specific probes designed using Junction Sequence design technology
  • Ideal for use with challenging sample types including FFPE tissue
  • Customizable and forward compatible with our growing line of ncounter® Vantage 3D™ Assays

Feature

Specifications

Input Material

50–100 ng Purified Total RNA (~5000–10,000 cells) 150–300 ng FFPE extracted RNA

Hands On Time

~15 min

Time to Results

Approximately 24 hrs

Sample Type

FFPE, fresh frozen tissue, cell extracts, cell lysates

Customizable Features

Add up to 24 additional genes or additional fusions with the nCounter TagSet Extension product.


Data Analysis

nSolver™ Analysis software


Required Reagents

MAX or FLEX: Panel CodeSet and Master Kit

SPRINT: Panel CodeSet and SPRINT Cartridge and Reagent Pack


The Vantage 3D Lung Fusion Panel includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes.

Specific Lung Gene Fusion Probes detect the following gene fusion families:


ALK

RET

ROS

NTRK1

EML4-ALK

CCDC6-RET

CD74-ROS1

CD74-NTRK1

HIP1-ALK

KIF5B-RET

EZR-ROS1

MPRIP-NTRK1

KIF5B-ALK

GOPC-ROS1

TFG-ALK

LRIG3-ROS1

TPR-ALK

SDC4-ROS1


SLC34A2-ROS1


TPM3-ROS1


Lung Imbalance probes detect gene expression imbalance in the following genes:


ALK

RET

ROS

8 probes

8 probes

8 probes


The Vantage 3D Leukemia Fusion Panel includes 42 probes in total: 27 for specific fusion detection, 12 leukemia genes, and 3 internal reference genes.

Leukemia Gene Fusion Probes detect the following gene fusion families:

CML

ALL

AML

AML/APL

BCR-ABL

E2A-PBX1

AML-ETO

PML-RARA


MLL-AF4

CBFB-MYH11



TEL-AML1

DEK-NUP214




RPN1-EVI1



Leukemia Gene Expression Probes:

Genes for AML, CML, and ALL

BAALC

ERG

FLT3

MECOM

MLLT11

MN1

NRAS

PRAME

RB1

SOCS2

TP53

WT1


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Product

Description

Catalog Number

Unit Size

nCounter® Vantage 3D™ Lung Gene Fusion Panel CodeSet Only

nCounter® Vantage 3D™ Leukemia Gene Fusion Panel CodeSet Only

Gene Expression CodeSet profiling (594 genes)

579 immunology-related human genes + 15 internal reference controls.

Master Kit reagents sold separately.

XT-CSO-HIM2-12

12 reactions

nCounter® Mouse Immunology Panel (CodeSet Only)

Gene Expression CodeSet profiling (561 genes)

547 immunology-related mouse genes + 14 internal reference controls.

Master Kit reagents sold separately.

XT-CSO-MIM1-12

12 reactions

For Research Use Only. Not for use in diagnostic procedures.