Gain a holistic view of the biology of the tumor, microenvironment, and immune response with an emphasis on dysfunctional cell signaling in cancer. Identify targets for novel therapies and understand the mechanism of action for current ones with a comprehensive look at 40+ pathways involved in tumor biology, immune evasion, and remodeling of the microenvironment.
- Profile altered signaling pathways in cancer
- Identify targets for novel therapeutics
- Understand the mechanism of action of targeted therapies
- Determine extent of anti-tumor immune response with the Tumor Inflammation Signature
- Quantify the presence and relative abundance of 14 different immune cell types
|Cellular Energetics (Hs/Mm)||Sustained Proliferation (Hs/Mm)||Evading Growth Suppressors (Hs/Mm)||Enabling Replicative Immortality (Hs/Mm)||Genomic Instability & Mutation (Hs/Mm)||Resisting Cell Death (Hs/Mm)|
|105/104 Genes||223/221 Genes||79/79 Genes||48/48 Genes||111/111 Genes||23/23 Genes|
|Autophagy||Androgen Signaling||Cell Cycle||Immortality & Stemness||DNA Damage & Repair||Apoptosis|
|Glucose Metabolism||EGFR Signaling||Senescence||Epigenetic & Transcriptional Regulation||TNF Superfamily|
|Glutamine Metabolism||ERBB2 Signaling||p53 Signaling|
|Lipid Metabolism||Estrogen Signaling|
|mTOR Signaling||FGFR Signaling|
|Nrf2 & Oxidative Stress||Hedgehog|
|Immune Evasion (Hs/Mm)||Tumor-Promoting Inflammation (Hs/Mm)||Activating Invasion and Metastasis (Hs/Mm)||Angiogenesis (Hs/Mm)|
|122/124 Genes||178/171 Genes||146/146 Genes||67/67 Genes|
|Antigen Presentation||Chemokine Signaling||Cell Adhesion & Motility||HIF1 Signaling|
|B cell Function||Inflammation||ECM Remodeling & Metastasis||PDGF Signaling|
|Cytotoxicity||Interferon Response||EMT||VEGF Signaling|
|Myeloid Immune Evasion||JAK-STAT Signaling||Hippo Signaling|
|T cell Co-stimulation||NF-kB Signaling|
|T cell Exhaustion|
The Tumor Inflammation Signature includes 18 functional genes known to be associated with response to PD-1/PD-L1 inhibitors.
Includes four areas of Immune Biology: IFN-ү-responsive genes related to antigen presentation, chemokine expression, cytotoxic activity, and adaptive immune resistance genes.
The tumor inflammation gene expression signature highlights the complex biology of the host immune microenvironment.
|18-Gene Tumor Inflammation Signature|
1. Ayers, Mark, et al. "IFN-y-related mRNA profile predicts clinical response to PD-1 blockade." The Journal of Clinical Investigation 127.8 (2017).
|B Cells||B cells are the primary mediators of the humoral immune response, bearing antigen-specific B cell receptors and producing antibodies that can enable the immune system to respond to a broad variety of antigens. B cells can also function as MHC class II antigen presenting cells to stimulate T cell immunity.|
|T Cells||T-cells mediate cell-based immunity by recognizing primarily peptide antigens displayed on MHC class I or class II and either producing cytokines or directly killing the presenting cell.|
|TH1||CD4+ T cell subset that produces IL2 and Interferon-gamma to promote cellular immunity by acting on CD8+ T Cells, NK Cells and Macrophages|
|Regulatory T Cells (Tregs)||CD4+ T Cells that suppress effector B and T Cells and play a central role in suppression of the immune response and tolerance to self-antigens|
|CD8+ T Cells||A subset of T cells that are capable of binding cognate-antigen expressing cells via class I MHC and directly lysing them via perforin and granzymes.|
|Exhausted CD8+ T Cells||T-cells overstimulated by antigen can develop an "exhausted" phenotype, in which they are no longer effective in targeting antigen-bearing cells.|
|Cytotoxic Cells||All cells capable of cytotoxic activity, which can include T, NKT, and NK-cells.|
|Dendritic Cells||Professional antigen presenting cells that internalize, process, and present antigens to lymphocytes via MHC class I and class II along with costimulatory signals to initiate cellular immune responses.|
|Macrophages||Pluripotent cells with critical roles in initiating innate and adaptive immune responses, phagocytosing abnormal cells, and regulating wound healing and tissue repair.|
|Mast Cells||Mast cells release histamine containing granules and other signals in order to promote inflammation and regulate allergic responses.|
|Neutrophils||Neutrophils are highly abundant cells that respond early to sites of infection or inflammation, phagocytose cellular debris, and promote downstream immunity.|
|Natural Killer (NK) Cells||Cytotoxic cells of the innate immune system that are a significant source of interferon-gamma and are capable of directly killing targeted cells via detection of a loss in MHC surface expression.|
|NK CD56dim cells||The amount of CD56 present on an NK cell is indicative of its age and differentiation state; CD56 dim cells are mature NK cells, more commonly found in peripheral blood than secondary lymphoid tissues and have the greatest cytolytic activity.|
|Number of Targets||780 (Human or Mouse), including internal reference genes|
|Sample Input - Standard (No amplification required)||25-300 ng|
|Sample Input - Low Input||As little as 1 ng with nCounter Low Input Kit (sold separately)|
|Sample Type(s)||Cultured cells/cell lysates, sorted cells, FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, and whole blood/plasma|
|Customizable||Add up to 30 unique genes with Panel-Plus and up to 10 custom protein targets|
|Time to Results||Approximately 24 hours|
|Data Analysis||nSolver™ Analysis Software (RUO)|
- Danaher, P et al. Gene Expression Markers of Tumor Infiltrating Leukocytes. J Immunother Cancer. 2017;21(5):18.
- Bieging KT and Attardi LD. Deconstructing p53 Transcriptional Networks in Tumor Suppression. Trends Cell Biol. 2012; 22 (2), 97-106.
- Bild, AH et al. Oncogenic Pathway Signatures in Human Cancers as a Guide to Targeted Therapies. Nature. 2006;439 (7074), 353-7.
- Chiaradonna, F. Ras-dependent Carbon Metabolism and Transformation in Mouse Fibroblasts. Oncogene. 2006;25 (39), 5391-404.
- Cordenonsi, M et al. The Hippo Transducer TAZ Confers Cancer Stem Cell-Related Traits on Breast Cancer Cells. Cell. 2011;147(4):759-72.
- Hanahan D and Weinberg, RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646-74.
- Jia, P and Zhao Z. Characterization of Tumor-Suppressor Gene Inactivation Events in 33 Cancer Types. Cell Reports. 2019;26, 496–506.
- Malta, TM et al. Machine Learning Identifies Stemness Features Associated With Oncogenic Dedifferentiation. Cell. 2018;173(2): 338-354.e15.
- Sanchez-Vega F et al. Oncogenic Signaling Pathways in The Cancer Genome Atlas. Cell. 2018; 173 (2), 321-337.e10.
- Semenza, GL Hypoxia-inducible Factor 1: Oxygen Homeostasis and Disease Pathophysiology. Trends Mol Med. 2001;7(8):345-50.
- Singh, A et al. RNAi-mediated Silencing of Nuclear Factor erythroid-2-related Factor 2 Gene Expression in Non-Small Cell Lung Cancer Inhibits Tumor Growth and Increases Efficacy of Chemotherapy. Cancer Res. 2008; 68 (19), 7975-84.
- Sun, J et al. A systematic analysis of FDA-approved anticancer drugs. BMC Systems Biology. 2017, 11(Suppl 5):87.
- Sweet-Cordero A et al. An Oncogenic KRAS2 Expression Signature Identified by Cross-Species Gene-Expression Analysis. Nat Genet. 2005; 37 (1), 48-55.
- Way, GP et al. Machine Learning Detects Pan-cancer Ras Pathway Activation in The Cancer Genome Atlas. Cell Rep. 2018;23(1): 172-180.e3.
|Product||Product Description||Quantity||Catalog Number|
|nCounter Human Tumor Signaling 360 Panel||Includes 780 genes;20 internal reference genes for data normalization||12 Reactions||XT-CSO-H Tumor Signaling 360-12|
|nCounter Mouse Tumor Signaling 360 Panel||Includes 780 genes;20 internal reference genes for data normalization||12 Reactions||XT-CSO-M Tumor Signaling 360-12|
|nCounter Master Kit (MAX or FLEX Systems) Reagents and Cartridges||Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems||12 Reactions||NAA-AKIT-012|
|nCounter SPRINT Cartridge 1 Cartridge, 12 lanes||Sample Cartridge for nCounter SPRINT System||12 Reactions||SPRINT-CAR-1.0|
|nCounter SPRINT Reagent Pack||nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer||192 Reactions||SPRINT-REAG-KIT|
For Research Use Only. Not for use in diagnostic procedures.