nCounter® Alzheimer's Disease Panel Mouse AD and Human AD

 

Reproducibly translate mouse models of Alzheimer’s disease (AD) to human disease with 770 standardized genes covering 30 clinically derived AD-associated modules discovered for AMP-AD consortium study of human brain tissue. Reliably assay AD phenotypes and disease progression for mouse model development and human tissue screening. Perform in vivo screening of potential therapeutics with an efficient and translational workflow to potentially reduce time to the clinic.

  • IM_MK1130_Neuro_MouseAD_Wheel_r6.pngMonitor disease progression with age
  • Assay novel therapeutics
  • Characterize AD
  • Customizable with up to 30 additional user-defined genes with Panel Plus option

Learn about our nCounter Analysis System and nSolver™ Analysis Software.

 

nSolver Advanced Analysis Modules available for Mouse AD and Human AD include:

  • Pathway Based Module Analysis
  • Pathway Analysis
  • Normalization
  • Quality Control
  • Differential Expression
  • Gene Set Analysis
  • Built-in compatibility for Panel Plus and Protein analysis

 

New Insights into Neuroscience Research

Explore this publication review of top neuroscience studies to see how gene expression technology is reshaping research capabilities in neuroscience.

Insights into Neuroscience Research Using nCounter Technology

AMP-AD consortium study

Read "Heterogeneity across human AD coexpression modules identified by meta-analysis of the human brain transcriptome”

Heterogeneity across human AD coexpression modules identified by meta-analysis of the human brain transcriptome
Module Enriched Pathways and Processes Represented Mouse/Human Genes*
CBEblue Transcription and Splicing 176
CBEbrown Lipid Metabolism, Myelination 95
CBEturquoise Cytokines 199
CBEyellow Vesicle Trafficking, Transmitter Synthesis and Storage 156/157
DLPFCblue Cytokines 182
DLPFCbrown Lipid Metabolism, Myelination 139
DLPFCturquoise Transcription and Splicing 143
DLPFCyellow Transmitter Synthesis and Storage, Vesicle Trafficking, Transmitter Release, Neural Connectivity  191/193
FPblue Lipid Metabolism, Myelination 277
FPbrown 77
FPturquoise Cytokines 107
FPyellow Vesicle Trafficking, Transmitter Synthesis and Storage, Neural Connectivity, Transmitter Release 187/188
IFGblue Transcription and Splicing, Lipid Metabolism, Myelination 236
IFGbrown Transmitter Synthesis and Storage, Vesicle Trafficking, Transmitter Release, Neural Connectivity 232/235
IFGturquoise Apoptosis, Cytokines 126
IFGyellow 84
PHGblue Transcription and Splicing 170
PHGbrown Transmitter Synthesis and Storage, Vesicle Trafficking 164/165
PHGgreen Lipid Metabolism, Myelination 139
PHGturquoise Cytokines 104
PHGyellow 101
STGblue Cytokines 143
STGbrown Transmitter Synthesis and Storage, Vesicle Trafficking, Neural Connectivity, Transmitter Release, Transmitter Response and Reuptake 210/212
STGturquoise Transcription and Splicing 118
STGyellow Lipid Metabolism, Myelination 159
TCXblue 118
TCXbrown Transcription and Splicing 124
TCXgreen Transmitter Synthesis and Storage, Vesicle Trafficking 185/187
TCXturquoise 78
TCXyellow Lipid Metabolism 152
Internal Reference Genes 10

† Annotations for 23 fundamental pathways and processes were assigned across all genes in the Mouse AD and Human AD panel allowing for an additional view of important aspects of the onset and progression of neurodegenerative disease. Pathways and processes with >60% representative gene content per module are listed above. Additional annotations can be found in the complete gene list.

‡ <60% representative pathway and process gene content per module

* Genes selected based on human-mouse gene homology, maximal coverage of AMP-AD modules, top AGORA candidate gene status (agora.ampadportal.org), representation in AMP-AD module eigengenes, and expression in mouse brain.

FEATURE SPECIFICATIONS
Number of Targets 770 including internal reference genes
Standard Input Material (No amplification required) 25 ng-300 ng
Sample Type(s) FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, whole blood/plasma, iPS cells
Species Mouse and Human
Customizable Add up to 30 unique genes with Panel-Plus
Time to Results Approximately 24 hours
Data Analysis nSolver™ Analysis Software, Advanced Analysis Modules

 

Selected Panel References:

1. ROSMAP: Bennett DA, et al. Overview and Findings from the Religious Orders Study. Current Alzheimer Research. 2012; 9(6): 628-45.

2. Mayo: Allen M et al. Human whole genome genotype and transcriptome data for Alzheimer’s and other neurodegenerative diseases. Scientific Data. 2016; 3.

3. MSBB: Wang M et al. The Mount Sinai cohort of large-scale genomic, transcriptomic and proteomic data in Alzheimer’s disease. Scientific Data. 2018; 5.

4. Logsdon BA, Perumal T, et al. Heterogeneity across human AD coexpression modules identified by meta-analysis of the human brain transcriptome. bioRxiv. 2019; 510420.

5. AMP-AD Knowledge Portal (www.ampadportal.org)

6. Carter et al. Translational Genetic and Genomic Analyses of New Mouse Models of Alzheimer’s Disease. Neuroscience 2018.

 

nCounter Neuroscience Publications

Click here to view nCounter Neuroscience Publications.

Product Product Description Quantity Catalogue Number
nCounter® Mouse AD Panel Includes 770 genes, including 10 internal reference genes for data normalization 12 reactions XT-CSO-MAD1-12
nCounter® Human AD Panel Includes 770 genes, including 10 internal reference genes for data normalization 12 reactions XT-CSO-HAD1-12

 

For Research Use Only. Not for use in diagnostic procedures.