nCounter® Immune Exhaustion Panel

Uncover the mechanisms behind T cell, B cell, and NK cell exhaustion in diverse contexts, including cancer and infectious disease, with a 785 gene panel that gets you results in less than 24 hours and is compatible with a broad range of sample types. Characterize immune status, develop signatures for assessing the exhausted state, and identify novel therapeutic targets to prevent or reverse exhaustion.

Product Highlights

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  • Directly profile 785 genes across 47 pathways involved in immune exhaustion:
    • Immune Activation
    • Immune Suppression
    • Immune Status
    • Immune Checkpoints
    • Epigenetics
    • Metabolism & Microenvironment
  • Understand the mechanisms of exhaustion in T cells, B cells, NK cells, CAR-T cells and other adoptive immune cells
  • Discover novel therapeutic targets for preventing or reversing immune exhaustion
  • Determine the extent of a peripherally suppressed, adaptive immune response to cancer with the 18-gene Tumor Inflammation Signature (TIS)
  • Quantify the presence and relative abundance of 14 different immune cell types

The nCounter Immune Exhaustion Panel enables researchers to explore the mechanisms behind T cell, B cell, and NK cell exhaustion in diverse contexts, including cancer and infectious disease.

Immune Activation Immune Suppression Immune Checkpoints Immune Status Epigenetics Metabolism & Microenvironment
Antigen Presentation IL-1 Signaling NK Receptors IL-10 Signaling CTLA 4 Signaling Anergy Epigenetic Modification Fatty Acid Metabolism
AP-1 Signaling IL-2 Signaling Other Interleukin Signaling Myeloid Immune Evasion PD1 Signaling B Cell Exhaustion   Glutamine Metabolism
Apoptosis IL-6 Signaling PI3K-AKT Pathway Notch Signaling T Cell Checkpoint Signaling Naïve   Glycolysis and Glucose Import
B Cell memory IL-7 Signaling T Cell memory RAR Signaling   NK Exhaustion   Hypoxia Response
BCR Signaling JAK/STAT Signaling TCR Signaling TGF-beta Signaling   Senescence & Quiescence   PPAR signaling
Cell Cycle MAPK Signaling TLR Signaling     T Cell Exhaustion    
Chemokine Signaling mTOR Signaling TNF Signaling          
Cytotoxicity NF-kB Signaling Type I Interferon          
DAP12 Signaling NK Activity Type II Interferon          

The Immune Exhaustion Panel provides comprehensive coverage of the most relevant immune checkpoints that can potentially be used to modulate the dynamics of the immune response.

CD28 ICOSLG LAG3 TNFSF18 ICOS
CD40 PDCD1 HAVCR2 VSIR CD70
CD80 TNFSF4 TNFSF9 CD27 CD276
CD86 CD274 TNFRSF9 CD40LG ADORA2A
CTLA4 PDCD1LG2 TNFRSF18 TNFRSF4 TIGIT

Genes included in the Immune Exhaustion Panel provide unique cell profiling data to measure the relative abundance of 14 different immune cell types. The table below summarizes the genes included in each cell type signature, as qualified through biostatistical approaches and selected literature in the field of immunology.

Cell Type Genes
B cells BLK, CD19, FAM30A, FCRL2, MS4A1, PNOC, SPIB, TCL1A, TNFRSF17
CD45 PTPRC
CD8 T cells CD8A, CD8B
Cytotoxic cells CTSW, GNLY, GZMA, GZMB, GZMH, KLRB1, KLRD1, KLRK1, NKG7, PRF1
Dendritic cells CCL13, CD209, HSD11B1
Exhausted CD8 CD244, EOMES, LAG3, PTGER4
Macrophages CD163, CD68, CD84, MS4A4A
Mast cells CPA3, HDC, MS4A2, TPSAB1/B2
NK CD56dim cells IL21R, KIR2DL3/4, KIR3DL1/2
NK cells NCR1, XCL1/2
Neutrophils CEACAM3, CSF3R, FCAR, FCGR3A/B, FPR1, S100A12, SIGLEC5
T cells CD3D, CD3E, CD3G, CD6, SH2D1A, TRAT1
Th1 cells TBX21
Treg FOXP3

Chronic infections caused by viruses and other pathogens can induce immune exhaustion. The Human Immune Exhaustion Panel includes probes for Epstein-Barr virus (EBV) and Cytomegalovirus (CMV), and the Mouse Immune Exhaustion Panel includes probes for Lymphocytic Choriomeningitis (LCMV). The panel can be supplemented with up to 55 genes of your choice with a Panel Plus spike-in for studying exhaustion in the context of different types of infectious disease.

The 18-gene Tumor Inflammation Signature (TIS) is included in the panel gene list and measures activity known to be associated with PD-1/PD-L1 inhibitors. Customers have the option to purchase a standalone TIS report with the Immune Exhaustion Panel.

  • Includes four axes of biology that characterize a peripherally suppressed, adaptive immune response, including:
    • Antigen presenting cells
    • T cell/NK cell presence
    • IFNγ biology
    • T cell exhaustion
  • Tissue-of-origin agnostic (Pan-Cancer)
  • Potential surrogate for PD-L1 and mutational load in a research setting
Feature Specifications
Number of Targets 785 (Human and Mouse), including 12 internal reference genes
Sample Input - Standard (No amplification required) 25-300 ng
Sample Input - Low Input As little as 1 ng with nCounter Low Input Kit and Primer Pools (sold separately)
Sample Type(s) Cultured cells/cell lysates, sorted cells, FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, and whole blood/plasma
Customizable Add up to 55 unique genes with Panel-Plus and/or up to 10 custom protein targets
Time to Results Approximately 24 hours
Data Analysis nSolver™ Analysis Software
Product Product Description Quantity Catalog Number
nCounter Human Immune Exhaustion Panel Includes 785 genes; 12 internal reference genes for data normalization 12 Reactions XT-H-EXHAUST-12
nCounter Mouse Immune Exhaustion Panel Includes 785 genes; 12 internal reference genes for data normalization 12 Reactions XT-M-EXHAUST-12
Low RNA Input Kit Kit for use with all Low RNA Input Primer Pools 48 Reactions LOW-RNA-48
nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems 12 Reactions NAA-AKIT-012
nCounter SPRINT Cartridge 1 Cartridge, 12 lanes Sample Cartridge for nCounter SPRINT System 12 Reactions SPRINT-CAR-1.0
nCounter SPRINT Reagent Pack nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer 192 Reactions SPRINT-REAG-KIT

For Research Use Only. Not for use in diagnostic procedures.