Uncover the mechanisms behind T cell, B cell, and NK cell exhaustion in diverse contexts, including cancer and infectious disease, with a 785 gene panel that gets you results in less than 24 hours and is compatible with a broad range of sample types. Characterize immune status, develop signatures for assessing the exhausted state, and identify novel therapeutic targets to prevent or reverse exhaustion.
- Directly profile 785 genes across 47 pathways involved in immune exhaustion:
- Immune Activation
- Immune Suppression
- Immune Status
- Immune Checkpoints
- Metabolism & Microenvironment
- Understand the mechanisms of exhaustion in T cells, B cells, NK cells, CAR-T cells and other adoptive immune cells
- Discover novel therapeutic targets for preventing or reversing immune exhaustion
- Determine the extent of a peripherally suppressed, adaptive immune response to cancer with the 18-gene Tumor Inflammation Signature (TIS)
- Quantify the presence and relative abundance of 14 different immune cell types
The nCounter Immune Exhaustion Panel enables researchers to explore the mechanisms behind T cell, B cell, and NK cell exhaustion in diverse contexts, including cancer and infectious disease.
|Immune Activation||Immune Suppression||Immune Checkpoints||Immune Status||Epigenetics||Metabolism & Microenvironment|
|Antigen Presentation||IL-1 Signaling||NK Receptors||IL-10 Signaling||CTLA 4 Signaling||Anergy||Epigenetic Modification||Fatty Acid Metabolism|
|AP-1 Signaling||IL-2 Signaling||Other Interleukin Signaling||Myeloid Immune Evasion||PD1 Signaling||B Cell Exhaustion||Glutamine Metabolism|
|Apoptosis||IL-6 Signaling||PI3K-AKT Pathway||Notch Signaling||T Cell Checkpoint Signaling||Naïve||Glycolysis and Glucose Import|
|B Cell memory||IL-7 Signaling||T Cell memory||RAR Signaling||NK Exhaustion||Hypoxia Response|
|BCR Signaling||JAK/STAT Signaling||TCR Signaling||TGF-beta Signaling||Senescence & Quiescence||PPAR signaling|
|Cell Cycle||MAPK Signaling||TLR Signaling||T Cell Exhaustion|
|Chemokine Signaling||mTOR Signaling||TNF Signaling|
|Cytotoxicity||NF-kB Signaling||Type I Interferon|
|DAP12 Signaling||NK Activity||Type II Interferon|
The Immune Exhaustion Panel provides comprehensive coverage of the most relevant immune checkpoints that can potentially be used to modulate the dynamics of the immune response.
Genes included in the Immune Exhaustion Panel provide unique cell profiling data to measure the relative abundance of 14 different immune cell types. The table below summarizes the genes included in each cell type signature, as qualified through biostatistical approaches and selected literature in the field of immunology.
|B cells||BLK, CD19, FAM30A, FCRL2, MS4A1, PNOC, SPIB, TCL1A, TNFRSF17|
|CD8 T cells||CD8A, CD8B|
|Cytotoxic cells||CTSW, GNLY, GZMA, GZMB, GZMH, KLRB1, KLRD1, KLRK1, NKG7, PRF1|
|Dendritic cells||CCL13, CD209, HSD11B1|
|Exhausted CD8||CD244, EOMES, LAG3, PTGER4|
|Macrophages||CD163, CD68, CD84, MS4A4A|
|Mast cells||CPA3, HDC, MS4A2, TPSAB1/B2|
|NK CD56dim cells||IL21R, KIR2DL3/4, KIR3DL1/2|
|NK cells||NCR1, XCL1/2|
|Neutrophils||CEACAM3, CSF3R, FCAR, FCGR3A/B, FPR1, S100A12, SIGLEC5|
|T cells||CD3D, CD3E, CD3G, CD6, SH2D1A, TRAT1|
Chronic infections caused by viruses and other pathogens can induce immune exhaustion. The Human Immune Exhaustion Panel includes probes for Epstein-Barr virus (EBV) and Cytomegalovirus (CMV), and the Mouse Immune Exhaustion Panel includes probes for Lymphocytic Choriomeningitis (LCMV). The panel can be supplemented with up to 55 genes of your choice with a Panel Plus spike-in for studying exhaustion in the context of different types of infectious disease.
The 18-gene Tumor Inflammation Signature (TIS) is included in the panel gene list and measures activity known to be associated with PD-1/PD-L1 inhibitors. Customers have the option to purchase a standalone TIS report with the Immune Exhaustion Panel.
- Includes four axes of biology that characterize a peripherally suppressed, adaptive immune response, including:
- Antigen presenting cells
- T cell/NK cell presence
- IFNγ biology
- T cell exhaustion
- Tissue-of-origin agnostic (Pan-Cancer)
- Potential surrogate for PD-L1 and mutational load in a research setting
|Number of Targets||785 (Human and Mouse), including 12 internal reference genes|
|Sample Input - Standard (No amplification required)||25-300 ng|
|Sample Input - Low Input||As little as 1 ng with nCounter Low Input Kit and Primer Pools (sold separately)|
|Sample Type(s)||Cultured cells/cell lysates, sorted cells, FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, and whole blood/plasma|
|Customizable||Add up to 55 unique genes with Panel-Plus and/or up to 10 custom protein targets|
|Time to Results||Approximately 24 hours|
|Data Analysis||nSolver™ Analysis Software|
- Ascierto ML et al. Inherent transcriptional signatures of NK cells are associated with response to IFNα + rivabirin therapy in patients with Hepatitis C Virus. J Transl Med 2015; 13: 77.
- Baitsch L et al. Exhaustion of tumor-specific CD8+ T cells in metastases from melanoma patients. J. Clin. Invest. 2011;121(6):2350-60.
- Beldi-Ferchiou A et al. PD-1 mediates functional exhaustion of activated NK cells in patients with Kaposi sarcoma. Oncotarget. 2016;7(45):72961-72977.
- Jelicic K et al. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression. Nat. Immunol. 2013;14(12): 1256-1265.
- Jiang Y et al. T-cell exhaustion in the tumor microenvironment. Cell Death & Disease. 2015;6(6):e1792.
- Khan O et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature. 2019;571(7764):211-218.
- Moir S & Fauci AS. B-cell exhaustion in HIV infection: the role of immune activation. Current Opinion in HIV and AIDS 2014;9(5):472-7.
- Wherry EJ & Kurachi M. Molecular and cellular insights into T cell exhaustion. Nat Rev Immunol 2015;15(8):486-99.
|Product||Product Description||Quantity||Catalog Number|
|nCounter Human Immune Exhaustion Panel||Includes 785 genes; 12 internal reference genes for data normalization||12 Reactions||XT-H-EXHAUST-12|
|nCounter Mouse Immune Exhaustion Panel||Includes 785 genes; 12 internal reference genes for data normalization||12 Reactions||XT-M-EXHAUST-12|
|Low RNA Input Kit||Kit for use with all Low RNA Input Primer Pools||48 Reactions||LOW-RNA-48|
|nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges||Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems||12 Reactions||NAA-AKIT-012|
|nCounter SPRINT Cartridge 1 Cartridge, 12 lanes||Sample Cartridge for nCounter SPRINT System||12 Reactions||SPRINT-CAR-1.0|
|nCounter SPRINT Reagent Pack||nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer||192 Reactions||SPRINT-REAG-KIT|
For Research Use Only. Not for use in diagnostic procedures.