Study the phases and progression of infection across the five major components of the host response with the pathogen-agnostic content of the nCounter Host Response Panel. Rapidly advance knowledge of emerging infectious disease with experiments that take minutes to set-up and get results in less than 24 hours.
- Profile 785 human genes across 50+ pathways optimized for blood but suitable for all sample types
- Study the five elements of the host response
- Host Susceptibility
- Interferon Response
- Innate Immune Cell Activation
- Adaptive Immune Response
- Detect the presence of a pathogen and evaluate organ-specific tissue damage with a Panel Plus spike-in
- Develop signatures of host response dynamics
- Identify and validate biomarkers for disease severity
- Evaluate the effect of vaccines & therapies
Get off-the-shelf data analysis and publication-ready figures with the nSolver™ Analysis Software.
Biological Framework of the Host Response Panel
|Innate Immune Cell Activation
|Adaptive Immune Response
|Angiotensin System||ALPK1 Signaling||Chemokine Signaling||BCR Signaling||Angiotensin System|
|Virus-Host Interaction||DNA Sensing||Cytoxicity||Complement System||Apoptosis|
|Glycan Sensing||Host Defense Peptides||Immune Exhaustion||Autophagy|
|Inflammasomes||IL-1 Signaling||Immune Memory||Coagulation|
|Interferon Response Genes||IL-2 Signaling||Lymphocyte Trafficking||HIF1A Signaling|
|JAK/STAT Signaling||IL-6 Signaling||MHC Class I Antigen Presentation||Leukotriene and Prostaglandin Inflammation|
|MAPK Signaling||IL-17 Signaling||MHC Class II Antigen Presentation||Lysosomes|
|NLR Signaling||Mononuclear Cell Migration||Mononuclear Cell Migration||Oxidative Stress Response|
|RNA Sensing||Myeloid Activation||T cell Costimulation||Proteotoxic Stress|
|TLR Signaling||Myeloid Inflammation||TCR Signaling||Tissue Stress|
|TNF Signaling||NK Activity||TH1 Differentiation||TNF Signaling|
|Type I Interferon Signaling||NF-kappaB Signaling||TH2 Differentiation|
|Type II Interferon Signaling||NO Signaling||TH9 Differentiation|
|Type III Interferon Signaling||Other Interleukin Signaling||TH17 Differentiation|
|Oxidative Stress Response||Treg Differentiation|
Customize your research project by adding tissue or pathogen-specific probes to the Host Response Panel with a 30-gene Panel Plus. Add the off-the-shelf 20-gene Coronavirus Panel Plus to study SARS-CoV-2 and other coronaviruses or build your own Panel Plus gene list with transcripts specific for different tissue types. Mix and match transcripts from the pathogen of your choice and additional host tissue markers to add a Panel Plus to the Host Response Panel for studying the pathogenesis of various infectious diseases.
Add up to 30 tissue relevant genes as a Panel Plus to the Host Response Panel to study organ damage wrought by COVID-19. Download the COVID-19 Tissue Reference Gene List to get ideas on which genes to add for studying the effect of COVID-19 on the kidney, GI tract, heart, liver, endothelium, lung, and brain. Check back often for updates as the NanoString Bioinformatics team continually adds to and refines these reference gene lists. The content is mined from publications and represents genes associated multiple times in the literature as related to a tissue in the context of COVID-19, as well as the top ten genes associated with that tissue. The rationale for each gene’s inclusion and its presence or absence in the Host Response Panel is noted. Each gene list can be used alongside any panel, please contact Bioinformatics for more information on which genes may already be present in your panel of interest.
Take advantage of comprehensive coverage of the most relevant immune checkpoints to study modulation of the host immune response and subsequent inflammatory cascade.
|ADORA2A (A2aR)||CD70||HAVCR2 (TIM3)||CD274 (PD-L1)||TNFRSF9 (4-1BB)|
|CD27||CD80||ICOS||PDCD1LG2 (PD-L2)||TNFSF9 (4-1BBL)|
|CD28||CD86||ICOSLG (B7-H2)||TIGIT||TNFRSF18 (GITR)|
|CD40||CD276 (B7-H3)||LAG3||TNFRSF4 (OX40)||TNFSF18 (GITRL)|
|CD40LG||CTLA-4||PDCD1 (PD-1)||TNFSF4 (OX40L)||VSIR (VISTA)|
Probes included in the Host Response Panel have high homology to non-human primates, allowing for seamless comparative infectious disease research as well as preclinical studies. Percent identity is used to estimate likelihood of the probe functioning on non-human primate targets. Additional comparisons with other NHP species are available upon request.
|Number of Genes|
|% Identity||Cynomologus Macaque||Rhesus Macaque|
Genes included in the Host Response Panel provide unique cell profiling data to measure the relative abundance of 14 different immune cell types. The table below summarizes the genes included in each cell type signature, as qualified through biostatistical approaches and selected literature in the field
|Cell Type||Associated Human Genes|
|B cells||BLK, CD19, FAM30A, FCRL2, MS4A1, PNOC, SPIB, TCL1A, TNFRSF17|
|CD8 T cells||CD8A, CD8B|
|Cytotoxic cells||CTSW, GNLY, GZMA, GZMB, GZMH, KLRB1, KLRD1, KLRK1, NKG7, PRF1|
|Dendritic cells||CCL13, CD209, HSD11B1|
|Exhausted CD8 cells||CD244, EOMES, LAG3, PTGER4|
|Macrophages||CD163, CD68, CD84, MS4A4A|
|Mast cells||CPA3, HDC, MS4A2, TPSAB1/B2|
|NK CD56dim cells||IL21R, KIR2DL3, KIR3DL1/2|
|NK cells||NCR1, XCL1/2|
|Neutrophils||CEACAM3, CSF3R, FCAR, FCGR3A/B, FPR1, S100A12, SIGLEC5|
|T cells||CD3D, CD3E, CD3G, CD6, SH2D1A, TRAT1|
|Number of Targets||785 (Human), including 12 internal reference genes|
|Sample Input - Standard (No amplification required)||25-300 ng|
|Sample Input - Low Input||As little as 1 ng with nCounter Low Input Kit and Primer Pools (sold separately)|
|Sample Type(s)||Cultured cells/cell lysates, sorted cells, FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, and whole blood/plasma|
|Customizable||Add up to 30 unique genes with Panel-Plus and/or up to 10 custom protein targets|
|Time to Results||Approximately 24 hours|
|Data Analysis||nSolver™ Analysis Software (RUO)|
- Altman, MC et al. Development and Characterization of a Fixed Repertoire of Blood Transcriptome Modules Based on Co-expression Patterns Across Immunological States. (2020) doi:10.1101/525709.
- Borchers, AT et al. Respiratory Syncytial Virus—A Comprehensive Review. Clin Rev Allergy Immunol. 2013;45(3):331-79.
- Dandekar, AA and Perlmam, S. Immunopathogenesis of coronavirus infections: implications for SARS. Nat Rev Immunol. 2005;5(12):917-27.
- Kannan, M et al. Platelet activation markers in evaluation of thrombotic risk factors in various clinical settings. Blood Rev. 2019;37:100583.
- Ong, EZ et al. A Dynamic Immune Response Shapes COVID-19 Progression. Cell Host & Microbe 2020;27(6):879-882.e2.
- Razonable, RR. Antiviral Drugs for Viruses Other Than Human Immunodeficiency Virus. Mayo Clin Proc. 2011;86(10):1009-26.
- Singhania, A et al. The value of transcriptomics in advancing knowledge of the immune response and diagnosis in tuberculosis. Nat Immunol. 2018;19(11):1159-1168.
- Speranza, E et al. A conserved transcriptional response to intranasal Ebola virus exposure in nonhuman primates prior to onset of fever. Sci Transl Med. 2018;10(434):eaaq1016.
- Zhai, Y et al. Host Transcriptional Response to Influenza and Other Acute Respiratory Viral Infections –A Prospective Cohort Study. PLoS Pathog. 2015;11(6):e1004869.
|Product||Product Description||Quantity||Catalog Number|
|nCounter Human Host Response Panel||Includes 785 genes; 12 internal reference genes for data normalization||12 Reactions||XT-HHR-12|
|Coronavirus Panel Plus||20 Probe Panel Plus. Includes 9 probes targeting the SARS-CoV-2 virus, 1 probe for the human viral receptor ACE2, and 10 additional probes targeting SARS and other human coronaviruses||12 Reactions||CORONAPP-12|
|Low RNA Input Kit||Kit for use with all Low RNA Input Primer Pools||48 Reactions||LOW-RNA-48|
|nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges||Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems||12 Reactions||NAA-AKIT-012|
|nCounter SPRINT Cartridge 1 Cartridge, 12 lanes||Sample Cartridge for nCounter SPRINT System||12 Reactions||SPRINT-CAR-1.0|
|nCounter SPRINT Reagent Pack||nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer||192 Reactions||SPRINT-REAG-KIT|
For Research Use Only. Not for use in diagnostic procedures.