nCounter® PanCancer Immune Profiling Panel

Perform multiplex gene expression analysis in human or mouse with 770 genes from 14 different immune cell types, common checkpoint inhibitors, CT antigens, and genes covering both the adaptive and innate immune response.

  • IM_MK1056_PanCancer_ImmProf_Wheel_r2_FINAL.pngComprehensive profiling of the immune response optimized for immuno-oncology research
  • Identify tumor-infiltrating lymphocytes (TILs) for the tumor microenvironment
  • Assess mechanistic pathway activity for single or combination studies
  • Customizable with up to 30 additional user-defined genes with Panel Plus option
  • 3D-enabled for multi-analyte analysis with Vantage 3D™ Assays

Cell Type Description
B Cells B cells are the primary mediators of the humoral immune response, bearing antigen-specific B cell receptors and producing antibodies that can enable the immune system to respond to a broad variety of antigens. B cells can also function as MHC class II antigen presenting cells to stimulate T cell immunity.
T Cells T-cells mediate cell-based immunity by recognizing primarily peptide antigens displayed on MHC class I or class II and either producing cytokines or directly killing the presenting cell.
TH1 CD4+ T cell subset that produces IL2 and Interferon-gamma to promote cellular immunity by acting on CD8+ T Cells, NK Cells and Macrophages
Regulatory T cells (Treg) CD4+ T Cells that supress effector B and T Cells and play a central role in suppresion of the immune response and tolerance to self-antigens
CD45 CD45 is a common marker of all leukocytes, including B and T cells
CD8+ T Cells A subset of T cells that are capable of binding cognate-antigen expressing cells via class I MHC and directly lysing them via perforin and granzymes.
Exhausted CD8+ T Cells T-cells overstimulated by antigen can develop an "exhausted" phenotype, in which they are no longer effective in targeting antigen-bearing cells.
Cytotoxic Cells These markers measure all cells capable of cytotoxic activity, which can include T, NKT, and NK-cells.
Dendritic Cells Professional antigen presenting cells that internalize, process, and present antigens to lymphocytes via MHC class I and class II along with costimulatory signals to initiate cellular immune responses.
Macrophages Pluripotent cells with critical roles in initiating innate and adaptive immune responses, phagocytosing abnormal cells, and regulating wound healing and tissue repair.
Mast Cells Mast cells release histamine containing granules and other signals in order to promote inflammation and regulate allergic responses.
Neutrophils Neutrophils are highly abundant cells that respond early to sites of infection or inflammation, phagocytose cellular debris, and promote downstream immunity.
Natural Killer (NK) Cells Cytotoxic cells of the innate immune system that are a significant source of interferon-gamma and are capable of directly killing targeted cells via detection of a loss in MHC surface expression
NK CD56dim cells The amount of CD56 present on an NK cell is indicative of its age and differentiation state; CD56 dim cells are mature NK cells, more commonly found in peripheral blood than secondary lymphoid tissues, and have the greatest cytolytic activity.



Number of Targets

770 including up to 40 PanCancer reference genes

Input Material

25 ng–300 ng

Sample Type(s)

FFPE-derived RNA, total RNA, fragmented RNA, cell lysates


Human or Mouse

Single Cell/Low Input Material Protocol




Time to Results

<72 hours

Data Analysis

nSolver™ Analysis software

Advanced Data Analysis

Immune cell profiling module

Shipping and Storage Conditions


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Catalog Number

NAV Number

Unit Size

nCounter® PanCancer Immune Profiling Panel (CodeSet Only)

Gene Expression CodeSet profiling (770 genes) 730 cancer-related human genes + 40 internal reference controls. Master Kit reagents sold separately.



12 reactions

nCounter® PanCancer Mouse Immune Profiling

(CodeSet Only)

Gene Expression CodeSet profiling (770 genes) 750 cancer-related mouse genes + 20 internal reference controls. Master Kit reagents sold separately.



12 reactions

For Research Use Only. Not for use in diagnostic procedures.