Create a customized protein assay with the Vantage 3D™ Protein Barcoding Service. Your antibody of choice is barcoded with specific DNA oligonucleotides matched to unique color-coded molecular barcodes to enable direct quantification of your proteins of interest on the nCounter platform.
- Customize any Vantage 3D Protein panel with up to 3 additional spike-in protein probes
- Develop a full custom protein panel (up to 10-plex)
- Compatible for use in protein only or Vantage 3D assay formats
- Enabled for use with Digital Spatial Profiling System (currently in development)
In order to perform the barcoding service, your antibodies must meet the following criteria:
|
Concentration & Volume |
1 mg +/- 20% at a concentration ≥ 1 mg/mL |
|
Buffer |
Buffer formulation shall be 1x PBS |
|
Purity |
Acceptable purity must be without glycerol, BSA, or other proteins or amine containing buffer |
|
Source |
Antibodies must be sourced from mouse or rabbit; monoclonal antibodies are preferred when possible |
|
Validation |
Antibodies should be validated for the intended use (for example: validated in flow cytometry for cell-suspension based applications, western or ELISA for lysate-based applications, or IHC for FFPE applications) |
Click here to view RNA:Protein Profiling.
Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.
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Product |
Description |
Catalog Number |
Unit Size |
|
nCounter Vantage 3D Protein Barcoding Service |
Protein customization service for spike-in to Vantage 3D Protein panels or full custom protein panel development |
PBS-1AB |
1 each |
|
Cross Reactivity Testing |
For Spike-In Only: Service to determine custom antibody spike-in cross reactivity with Vantage 3D Protein panel targets |
PBS-XTEST |
Up to 3 each |
|
nCounter Vantage 3D Protein Barcoding Service TagSet (D) |
For Full Custom Only : Protein detection reagent to support a full custom stand-alone protein assay or full custom protein assay for use with nCounter Vantage 3D DNA. Not required for spike-in applications. |
PBS-POD-12 |
12 reactions |
|
nCounter Vantage 3D Protein Barcoding Service TagSet (R) |
For Full Custom Only : Protein detection reagent to support full custom protein use with RNA (+/-DNA) including custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Not required for spike-in applications. |
PBS-PXD-12 |
12 reactions |
For Research Use Only. Not for use in diagnostic procedures.