nCounter® Vantage 3D™ Protein Barcoding Service

Create a customized protein assay with the Vantage 3D™ Protein Barcoding Service. Your antibody of choice is barcoded with specific DNA oligonucleotides matched to unique color-coded molecular barcodes to enable direct quantification of your proteins of interest on the nCounter platform.

  • Customize any Vantage 3D Protein panel with up to 3 additional spike-in protein probes
  • Develop a full custom protein panel (up to 10-plex)
  • Compatible for use in protein only or Vantage 3D assay formats
  • Enabled for use with Digital Spatial Profiling System (currently in development)

In order to perform the barcoding service, your antibodies must meet the following criteria:

Concentration & Volume

1 mg +/- 20% at a concentration ≥ 1 mg/mL

Buffer

Buffer formulation shall be 1x PBS

Purity

Acceptable purity must be without glycerol, BSA, or other proteins or amine containing buffer

Source

Antibodies must be sourced from mouse or rabbit; monoclonal antibodies are preferred when possible

Validation

Antibodies should be validated for the intended use (for example: validated in flow cytometry for cell-suspension based applications, western or ELISA for lysate-based applications, or IHC for FFPE applications)

Click here to view RNA:Protein Profiling.

Click here to view Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.

Product

Description

Catalog Number

Unit Size

nCounter Vantage 3D Protein Barcoding Service

Protein customization service for spike-in to Vantage 3D Protein panels or full custom protein panel development

PBS-1AB

1 each

Cross Reactivity Testing

For Spike-In Only: Service to determine custom antibody spike-in cross reactivity with Vantage 3D Protein panel targets

PBS-XTEST

Up to 3 each

nCounter Vantage 3D Protein Barcoding Service TagSet (D)

For Full Custom Only : Protein detection reagent to support a full custom stand-alone protein assay or full custom protein assay for use with nCounter Vantage 3D DNA. Not required for spike-in applications.

PBS-POD-12

12 reactions

nCounter Vantage 3D Protein Barcoding Service TagSet (R)

For Full Custom Only : Protein detection reagent to support full custom protein use with RNA (+/-DNA) including custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Not required for spike-in applications.

PBS-PXD-12

12 reactions

For Research Use Only. Not for use in diagnostic procedures.