nCounter® Panel-Plus and CodeSet-Plus

Panel-Plus and CodeSet-Plus offer the most flexible solutions to customizing your nCounter Experiment. Customize any off-the-shelf panel product or add genes to any custom CodeSet by utilizing up to 30 unique Reporter Codes exclusively formulated for use in Panel-Plus and CodeSet-Plus products.

  • Customize any off-the-shelf Panel product by adding up to 30 of your favorite genes.
  • Update any Custom CodeSet previously purchased with new genes for your research.
  • Suitable for use with all Gene Expression and CNV products including Single Cell applications.
  • Universally compatible with all current and past custom CodeSets or Panels purchased for the nCounter system.

The Ultimate Flexibility for your nCounter Experiment

Panel-Plus and CodeSet-Plus offer the most flexible solutions to customizing your nCounter Experiment. Choose from several options to create a custom plus product by adding as few as 6 genes or as many as 30 genes. Send us your request below and the NanoString team of bioinformaticians will design your Plus product for you from a simple list of gene names.

To receive a quote, please fill out the Contact Us form

nCounter Panel-Plus

nCounter CodeSet-Plus
6-10 genes
11-20 genes
21-30 genes
48 reactions minimum
6-10 genes
11-20 genes
21-30 genes
48 reactions minimum

1. Can I use multiple Plus products with a Panel or Custom CodeSet?

No. Multiple Plus products cannot be mixed or combined in a reaction. Only one Plus product may be used in conjunction with a set of specific reactions. It is possible to use different Plus products on separate occasions with different Panels or CodeSets.

2. Can I use the Plus product as I would a custom CodeSet?

No. Plus products are not the same as custom CodeSets. Plus products have been formulated to be mixed with Panel or Custom CodeSet Reporter and Capture Probe Mixes. Plus products also do not include the necessary controls for proper normalization.

3. How do I include the new genes in my data analysis?

To include the new genes, please contact NanoString Bioinformatics at bioinformatics@nanostring.com. Include in your message the name of the Plus product and original CodeSet that will be combined. A new Reporter Library File (RLF) containing both will be sent within 24 hours.

4. How do I analyze the combined data?

After scanning on the Digital Analyzer, data may be imported into nSolver™ Analysis Software for downstream analysis. Please see the nSolver Analysis Software Manual for additional information on the nSolver application.

5. Can I purchase a Panel-Plus for my miRNA, miRGE™, or Plex2 experiment?

Not at this time. Plus products are specially formulated to be universally compatible with all CNV and Gene Expression CodeSets

Performance of Panel-Plus Gene Sets with pre-designed Panels (Standard Gene Expression and Single Cell Analysis)

Two experiments were performed by adding Panel-Plus genes to the Cancer Reference Panel. Data in each figure below demonstrate that addition of CodeSet-Plus probes does not impact performance of original nCounter Panel probes or Single Cell Panel probes.

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100 ng) was hybridized with either nCounter Cancer Reference Panel (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the Original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100 pg) was converted to cDNA and enriched via a Multiplexed Target Enrichment (MTE) with primers appropriate for downstream hybridization according to the nCounter Single Cell Expression Assay Protocol for Total RNA. The enriched cDNA was hybridized with either nCounter Cancer Reference Panel CodeSet (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

For Research Use Only. Not for use in diagnostic procedures.