nCounter® PlexSet™ Reagents

Download PlexSet Preselected Pathway Panels

 

Accelerate your cell line screening and other high-throughput applications with nCounter PlexSet Reagents for Gene Expression Analysis.

Download the Product Bulletin and Reagents User Manual today!

  • Multiplex up to 96 custom probes (or choose from preselected pathway panels) across 96 samples to generate 9,216 data points per run with only 30 minutes of hands-on time
  • Utilization of lysed cells eliminates the need for RNA purification and amplification; save resources with an efficient workflow that does not require cDNA conversions*, replicates, or prior probe optimizations
  • Proven nCounter Digital Gene Expression technology provides excellent precision and reproducibility across a wide dynamic range
  • Turn-key; custom reagents do not require upfront optimization prior to initiating a new study

*Needed if working with single cells

 

Feature Specifications
Number of Targets Up to 96 targets (provided by users to bioinformatics@nanostring.com) or choose from the preselected pathway panels; 4 internal controls already included
Minimal Input Material 50 ng of total purified RNA or 5,000 cells or 1 ng of RNA input with amplification
Sample Type(s) Total purified RNA (cells or tissues), cell lysates, Fresh frozen or FFPE-derived RNA, amplified RNA
Species Any
Time to Results ~30 minutes - hands on time; <24 hours - time to results
Data Analysis nSolver™ Analysis software
Shipping and Storage Conditions -80ºC (Hyb buffer kit at 4ºC)

Utilization of the lysed cells eliminates the need for RNA purification

Above data demonstrate PlexSet Technology showing high concordance between lysed cells and purified RNA. PlexSet enables direct use of lysed cell samples in the experiment to attain highly specific and sensitive gene expression analysis for high sample multiplexing.

Click here to view publications.

Product Description Catalog Number Unit Size
nCounter PlexSet-12 Reagent Pack  For custom GX analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only- does not include Master Kits. PS-012-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-12 Kit is set up to perform titration for 12 gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-12 (CSO) 24 reactions
nCounter PlexSet-24 Reagent Pack For custom GX analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-024-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-24 Kit is set up to perform titration for 24 gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-24 (CSO) 24 reactions
nCounter PlexSet-48 Reagent Pack For custom GX analysis utilizing 48 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-048-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-48 Kit is set up to perform titration for 48 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-48 (CSO) 24 reactions
nCounter PlexSet-72 Reagent Pack For custom GX analysis utilizing 72 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-072-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-72 Kit is set up to perform titration for 72 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-72 (CSO) 24 reactions
nCounter PlexSet-96 Reagent Pack For custom GX analysis utilizing 96 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-096-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-96 Kit is set up to perform titration for 96 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-96 (CSO) 24 reactions

 

PlexSet Preselected Pathway Panels

Expertly curated panels covering ~140 biological pathways and fields of interest for human, mouse, and rat samples. Each panel contains 90 genes chosen to comprehensively cover each pathway involved in the topic. All necessary controls and reference genes are included in each panel. Customize by adding additional genes of interest to the panels (or omitting genes that are not of interest). By utilizing PlexSet reagents with these panels up to 96 samples can be run in a single nCounter run.

Panels    

 

 
Adherens Junctions Adipose Differentiation and Maintenance Allergic Response Alzheimer's Disease AMPK Pathway
Androgen Receptor Target Genes Angiogenesis Angiogenic Signaling Antigen Presenting Cell Apoptosis
Atherosclerosis Autoimmunity Autophagy Blood Pressure RegulationRat Breast Cancer
Calcium Signaling Cancer Driver Genes Cancer Stem Cell Pathways Cardiovascular Disease CD4+ T-cell Differentiation
Cell Cycle Cell Death Cell Migration Cell Stress and Toxicity Cell-Matrix Interaction
Cellular Basis of Aging Cellular Senescence Cellular Stress Chemokine SignalingRat Chemotherapy Resistance
Cholesterol and Lipoprotein Metabolism Chromatin-modifying Enzymes Chromatin-remodeling Complexes Common Signaling Pathways Cytokines
Cytokines and Chemokines Cytoskeleton Regulation Detoxification Diabetes DNA Damage Repair
DNA Damage Signaling Drug Transporters EGF and PDGF Signaling Endothelial Cell Function Epithelial Cell Junctions
Epithelial to Mesenchymal Transition (EMT) Essential Amino Acid Metabolism Estrogen Receptor Signaling Fatty Acid Metabolism Fatty Liver Disease
Fibrosis Focal Adhesion Signaling Gap Junctions Glucocorticoid Signaling Glycosylation
GPCR Pathways Growth Factors Hedgehog Signaling Hematopoiesis Hippo Pathway
Huntington's Disease Hypoxia Signaling Immune response to bacterial infection Immune response to fungal infection Immune Response to tumors
Immune response to viral infection Immune Tolerance Immunotoxicity Induced Pluripotent Stem (IPS) Cells Inflammasome Complexes and Regulation
Inflammatory Cytokine Signaling Insulin Signaling Interferon Signaling IO Lymphocyte Activity IO Myeloid Activity
Irritable Bowel Disease Kidney Toxicity Learning and Memory Leukemia Liver Cancer
Liver Toxicity Lung Cancer Lymphocyte Activation Lymphoma MAPK Pathway
Mesenchymal Stem Cells Metastasis Mitochondrial biogenesis and function Molecular Toxicology mTOR Pathway
Multiple Sclerosis Myc Target Genes Necrosis Neurotransmitter Receptors Neurotrophin Signaling
NF-kB Pathway NF-kB Target Genes Nitric Oxide Signaling Non-essential Amino Acid Metabolism Notch Pathway
Notch Target Genes Obesity Oncology Drug Targets Osmotic Stress Osteogenesis
Osteoporosis Oxidative Stress p53 Pathway Parkinson's Disease Phagocytosis
Phase II Drug Metabolism Phosphatases PI3K-Akt Pathway Polycomb and Trithorax Complexes Polycomb and Trithorax Target Genes
Prostate Cancer Protein folding Retinoic Acid Signaling Sepsis Skeletal Myogenesis and Myopathies
Stem Cell Signaling Stem Cell Transcription Factors Stem Cells Telomere Replication and Maintenance TGF-b Pathway
TGF-b Target Genes Th1 and Th2 Responses Th17 Response Tight Junctions TNF Signaling
Toll-Like Receptor Pathway Transcription Factors Type I Interferon Signaling Type II Diabetes Ubiquitination
Unfolded Protein Response VEGF Pathway WNT Pathway WNT Target Genes Wound Healing

For Research Use Only. Not for use in diagnostic procedures.