Simple, cost-effective multiplex gene expression analysis for high throughput research applications.
- Multiplex up to 24 custom probes across 96 samples to generate 2,304 data points per run with only 30 minutes of hands-on time
- “Lyse-and-go” protocol for cells eliminates the need for RNA purification and amplification
- Proven nCounter Digital Gene Expression technology provides excellent precision and reproducibility across a wide dynamic range
- Turn-key; custom reagents do not require upfront optimization prior to initiating a new study
- Save resources with an efficient workflow that does not require cDNA conversions*, replicates, and prior probe optimizations
*Needed if working with single cells
Simple "lyse-and-go" protocol for cell samples
The technology offers a simple workflow to lyse the cells with nCounter Buffer LH and to directly use them as samples in the experiment to attain highly specific and sensitive gene expression analysis using nSolver software. Highly optimized “Lyse and Go” protocol helps researchers save time and resources. Above we demonstrate data from the highly multiplexed PlexSet technology shows high concordance between lysed cells and purified RNA.
|Number of Targets||Up to 24 targets (provided by users to firstname.lastname@example.org); 4 internal controls already included|
|Minimal Input Material||50 ng of total purified RNA or 5,000 cells or 1 ng of RNA input with amplification|
|Sample Type(s)||Total purified RNA (cells or tissues), cell lysates, Fresh frozen or FFPE-derived RNA, amplified RNA|
|Time to Results||~30 minutes - hands on time; <24 hours - time to results|
|Data Analysis||nSolver™ Analysis software|
|Shipping and Storage Conditions||-80ºC (Hyb buffer kit at 4ºC)|
|Product||Description||Catalog Number||Unit Size|
|nCounter PlexSet-12 Reagent Pack||For custom GX analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master Kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only - does not include Master Kits.||PS-012-GX-192S (CSO)||192 reactions|
|nCounter PlexSet Titration Kit-12||Kit is set up to perform titration for 12 gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only - does not include Master Kits.||PS-GX-PTK-12 (CSO)||12 reactions|
|nCounter PlexSet-24 Reagent Pack||For custom GX analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master Kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only - does not include Master Kits.||PS-024-GX-192S (CSO)||192 reactions|
|nCounter PlexSet Titration Kit-24||Kit is set up to perform titration for 24 gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only - does not include Master Kits.||PS-GX-PTK-24 (CSO)||12 reactions|
|Buffer LH Bottle (50mL)||Buffer LH is a lysis buffer optimized to lyse cells and utilize them directly with PlexSet reagents. One 50mL bottle is sufficient to analyze samples for 8 plates (4 pack or 768 samples) of PlexSet reagents.||PS-GX-BLH||768 reactions|
For Research Use Only. Not for use in diagnostic procedures.