nCounter® PlexSet™ Reagents

Accelerate your cell line screening and other high-throughput applications with nCounter PlexSet Reagents for Gene Expression Analysis.

Download the Product Bulletin and Reagents User Manual today!

  • Multiplex up to 96 custom probes (or choose from preselected pathway panels) across 96 samples to generate 9,216 data points per run with only 30 minutes of hands-on time
  • Utilization of lysed cells eliminates the need for RNA purification and amplification; save resources with an efficient workflow that does not require cDNA conversions*, replicates, or prior probe optimizations
  • Proven nCounter Digital Gene Expression technology provides excellent precision and reproducibility across a wide dynamic range
  • Turn-key; custom reagents do not require upfront optimization prior to initiating a new study

*Needed if working with single cells

 

Feature Specifications
Number of Targets Up to 96 targets (provided by users to bioinformatics@nanostring.com) or choose from the preselected pathway panels; 4 internal controls already included
Minimal Input Material 50 ng of total purified RNA or 5,000 cells or 1 ng of RNA input with amplification
Sample Type(s) Total purified RNA (cells or tissues), cell lysates, Fresh frozen or FFPE-derived RNA, amplified RNA
Species Any
Time to Results ~30 minutes - hands on time; <24 hours - time to results
Data Analysis nSolver™ Analysis software
Shipping and Storage Conditions -80ºC (Hyb buffer kit at 4ºC)

Expertly curated panels covering ~140 biological pathways and fields of interest for human, mouse, and rat samples. Each panel contains 90 genes chosen to comprehensively cover each pathway involved in the topic. All necessary controls and reference genes are included in each panel. Customize by adding additional genes of interest to the panels (or omitting genes that are not of interest). By utilizing PlexSet reagents with these panels up to 96 samples can be run in a single nCounter run.

Panel Species Species

Species

(coming soon)

Any Other Species
Adherens Junctions Human Mouse Rat Request Other Species
Adipose Differentation and Maintenance Human Mouse Rat Request Other Species
Allergic Response Human Mouse Rat Request Other Species
Alzheimer's Disease Human Mouse Rat Request Other Species
AMPK Pathway Human Mouse Rat Request Other Species
Androgen Receptor Target Genes Human Mouse Rat Request Other Species
Angiogenesis Human Mouse Rat Request Other Species
Angiogenic Signaling Human Mouse Rat Request Other Species
Antigen Presenting Cell Human Mouse Rat Request Other Species
Apoptosis Human Mouse Rat Request Other Species
Atherosclerosis Human Mouse Rat Request Other Species
Autoimmunity Human Mouse Rat Request Other Species
Autophagy Human Mouse Rat Request Other Species
Blood Pressure Regulation Human Mouse Rat Request Other Species
Breast Cancer Human Mouse Rat Request Other Species
Calcium Signaling Human Mouse Rat Request Other Species
Cancer Driver Genes Human Mouse Rat Request Other Species
Cancer Stem Cell Pathways Human Mouse Rat Request Other Species
Cardiovascular Disease Human Mouse Rat Request Other Species
CD4+ T-cell Differentation Human Mouse Rat Request Other Species
Cell Cycle Human Mouse Rat Request Other Species
Cell Death Human Mouse Rat Request Other Species
Cell Migration Human Mouse Rat Request Other Species
Cell Stress and Toxicity Human Mouse Rat Request Other Species
Cell-Matrix Interaction Human Mouse Rat Request Other Species
Cellular Basis of Aging Human Mouse Rat Request Other Species
Cellular Senescence Human Mouse Rat Request Other Species
Cellular Stress Human Mouse Rat Request Other Species
Chemokine Signaling Human Mouse Rat Request Other Species
Chemotherapy Resistance Human Mouse Rat Request Other Species
Cholesterol and Lipoprotein Metabolism Human Mouse Rat Request Other Species
Chromatin-modifying Enzymes Human Mouse Rat Request Other Species
Chromatin-remodeling Complexes Human Mouse Rat Request Other Species
Common Signaling Pathways Human Mouse Rat Request Other Species
Cytokines Human Mouse Rat Request Other Species
Cytokines and Chemokines Human Mouse Rat Request Other Species
Cytoskeleton Regulation Human Mouse Rat Request Other Species
Detoxification Human Mouse Rat Request Other Species
Diabetes Human Mouse Rat Request Other Species
DNA Damage Repair Human Mouse Rat Request Other Species
DNA Damage Signaling Human Mouse Rat Request Other Species
Drug Transporters Human Mouse Rat Request Other Species
EGF and PDGF Signaling Human Mouse Rat Request Other Species
Endothelial Cell Function Human Mouse Rat Request Other Species
Epithelial Cell Junctions Human Mouse Rat Request Other Species
Epithelial to Mesenchymal Transition (EMT) Human Mouse Rat Request Other Species
Essential Amino Acid Metabolism Human Mouse Rat Request Other Species
Estrogen Receptor Signaling Human Mouse Rat Request Other Species
Fatty Acid Metabolism Human Mouse Rat Request Other Species
Fatty Liver Disease Human Mouse Rat Request Other Species
Fibrosis Human Mouse Rat Request Other Species
Focal Adhesion Signaling Human Mouse Rat Request Other Species
Gap Junctions Human Mouse Rat Request Other Species
Glucocorticoid Signaling Human Mouse Rat Request Other Species
Glycosylation Human Mouse Rat Request Other Species
GPCR Pathways Human Mouse Rat Request Other Species
Growth Factors Human Mouse Rat Request Other Species
Hedgehog Signaling Human Mouse Rat Request Other Species
Hematopoiesis Human Mouse Rat Request Other Species
Hippo Pathway Human Mouse Rat Request Other Species
Huntington's Disease Human Mouse Rat Request Other Species
Hypoxia Signaling Human Mouse Rat Request Other Species
Immune response to bacterial infection Human Mouse Rat Request Other Species
Immune response to fungal infection Human Mouse Rat Request Other Species
Immune Response to tumors Human Mouse Rat Request Other Species
Immune response to viral infection Human Mouse Rat Request Other Species
Immune Tolerance Human Mouse Rat Request Other Species
Immunotoxicity Human Mouse Rat Request Other Species
Induced Plurpotent Stem (IPS) Cells Human Mouse Rat Request Other Species
Inflammasome Complexes and Regulation Human Mouse Rat Request Other Species
Inflammatory Cytokine Signaling Human Mouse Rat Request Other Species
Insulin Signaling Human Mouse Rat Request Other Species
Interferon Signaling Human Mouse Rat Request Other Species
IO Lymphocyte Activity Human Mouse Rat Request Other Species
IO Myeloid Activity Human Mouse Rat Request Other Species
Irritable Bowel Disease Human Mouse Rat Request Other Species
Kidney Toxicity Human Mouse Rat Request Other Species
Learning and Memory Human Mouse Rat Request Other Species
Leukemia Human Mouse Rat Request Other Species
Liver Cancer Human Mouse Rat Request Other Species
Liver Toxicity Human Mouse Rat Request Other Species
Lung Cancer Human Mouse Rat Request Other Species
Lymphocyte Activation Human Mouse Rat Request Other Species
Lymphoma Human Mouse Rat Request Other Species
MAPK Pathway Human Mouse Rat Request Other Species
Mesenchymal Stem Cells Human Mouse Rat Request Other Species
Metastasis Human Mouse Rat Request Other Species
Mitochondrial biogenesis and function Human Mouse Rat Request Other Species
Molecular Toxicology Human Mouse Rat Request Other Species
mTOR Pathway Human Mouse Rat Request Other Species
Multiple Sclerosis Human Mouse Rat Request Other Species
Myc Target Genes Human Mouse Rat Request Other Species
Necrosis Human Mouse Rat Request Other Species
Neurotransmitter Receptors Human Mouse Rat Request Other Species
Neurotrophin Signaling Human Mouse Rat Request Other Species
NF-kB Pathway Human Mouse Rat Request Other Species
NF-kB Target Genes Human Mouse Rat Request Other Species
Nitric Oxide Signaling Human Mouse Rat Request Other Species
Non-essential Amino Acid Metabolism Human Mouse Rat Request Other Species
Notch Pathway Human Mouse Rat Request Other Species
Notch Target Genes Human Mouse Rat Request Other Species
Obesity Human Mouse Rat Request Other Species
Oncology Drug Targets Human Mouse Rat Request Other Species
Osmotic Stress Human Mouse Rat Request Other Species
Osteogenesis Human Mouse Rat Request Other Species
Osteoporosis Human Mouse Rat Request Other Species
Oxidative Stress Human Mouse Rat Request Other Species
p53 Pathway Human Mouse Rat Request Other Species
Parkinson's Disease Human Mouse Rat Request Other Species
Phagocytosis Human Mouse Rat Request Other Species
Phase II Drug Metabolism Human Mouse Rat Request Other Species
Phosphatases Human Mouse Rat Request Other Species
PI3K-Akt Pathway Human Mouse Rat Request Other Species
Polycomb and Trithorax Complexes Human Mouse Rat Request Other Species
Polycomb and Trithorax Target Genes Human Mouse Rat Request Other Species
Prostate Cancer Human Mouse Rat Request Other Species
Protein folding Human Mouse Rat Request Other Species
Retinoic Acid Signaling Human Mouse Rat Request Other Species
Sepsis Human Mouse Rat Request Other Species
Skeletal Myogenesis and Myopathies Human Mouse Rat Request Other Species
Stem Cell Signaling Human Mouse Rat Request Other Species
Stem Cell Transcription Factors Human Mouse Rat Request Other Species
Stem Cells Human Mouse Rat Request Other Species
Telomere Replication and Maintenance Human Mouse Rat Request Other Species
TGF-b Pathway Human Mouse Rat Request Other Species
TGF-b Target Genes Human Mouse Rat Request Other Species
Th1 and Th2 Responses Human Mouse Rat Request Other Species
Th17 Response Human Mouse Rat Request Other Species
Tight Junctions Human Mouse Rat Request Other Species
TNF Signaling Human Mouse Rat Request Other Species
Toll-Like Receptor Pathway Human Mouse Rat Request Other Species
Transcription Factors Human Mouse Rat Request Other Species
Type I Interferon Signaling Human Mouse Rat Request Other Species
Type II Diabetes Human Mouse Rat Request Other Species
Ubiquitination Human Mouse Rat Request Other Species
Unfolded Protein Response Human Mouse Rat Request Other Species
VEGF Pathway Human Mouse Rat Request Other Species
WNT Pathway Human Mouse Rat Request Other Species
WNT Target Genes Human Mouse Rat Request Other Species
Wound Healing Human Mouse Rat Request Other Species

Utilization of the lysed cells eliminates the need for RNA purification

Above data demonstrate PlexSet Technology showing high concordance between lysed cells and purified RNA. PlexSet enables direct use of lysed cell samples in the experiment to attain highly specific and sensitive gene expression analysis for high sample multiplexing.

Click here to view publications.

Product Description Catalog Number Unit Size
nCounter PlexSet-12 Reagent Pack  For custom GX analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only- does not include Master Kits. PS-012-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-12 Kit is set up to perform titration for 12 gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-12 (CSO) 24 reactions
nCounter PlexSet-24 Reagent Pack For custom GX analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-024-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-24 Kit is set up to perform titration for 24 gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-24 (CSO) 24 reactions
nCounter PlexSet-48 Reagent Pack For custom GX analysis utilizing 48 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-048-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-48 Kit is set up to perform titration for 48 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-48 (CSO) 24 reactions
nCounter PlexSet-72 Reagent Pack For custom GX analysis utilizing 72 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-072-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-72 Kit is set up to perform titration for 72 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-72 (CSO) 24 reactions
nCounter PlexSet-96 Reagent Pack For custom GX analysis utilizing 96 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet only- does not include Master Kits PS-096-GX-96S (CSO) 96 reactions
nCounter PlexSet Titration Kit-96 Kit is set up to perform titration for 96 gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-96 (CSO) 24 reactions

 

For Research Use Only. Not for use in diagnostic procedures.