CodeSet-Plus

CodeSet-Plus offers a flexible solution to customizing your nCounter® experiment. Customize any CodeSet previously ordered with new genes of your choice by adding 6 to 30 user-defined probes (universally compatible with all current and past nCounter CodeSets purchased).

Send us your request below and the NanoString Bioinformatics team will design your Plus product for you from a simple list of gene names.

To receive a quote, please fill out the Contact Us form.

ncounter codeset-plus
6-10 genes
11-20 genes
21-30 genes
48 reactions minimum

 

1. Can I use multiple Plus products with a Panel or Custom CodeSet?

No. Multiple Plus products cannot be mixed or combined in a reaction. Only one Plus product may be used in conjunction with a set of specific reactions. It is possible to use different Plus products on separate occasions with different Panels or CodeSets.

2. Can I use the Plus product as I would a custom CodeSet?

No. Plus products are not the same as custom CodeSets. Plus products have been formulated to be mixed with Panel or Custom CodeSet Reporter and Capture Probe Mixes. Plus products also do not include the necessary controls for proper normalization.

3. How do I include the new genes in my data analysis?

To include the new genes, please contact NanoString Bioinformatics at bioinformatics@nanostring.com. Include in your message the name of the Plus product and original CodeSet that will be combined. A new Reporter Library File (RLF) containing both will be sent within 24 hours.

4. How do I analyze the combined data?

After scanning on the Digital Analyzer, data may be imported into nSolver™ Analysis Software for downstream analysis. Please see the nSolver Analysis Software Manual for additional information on the nSolver application.

5. Can I purchase a Panel-Plus for my miRNA, miRGE™, or Plex2 experiment?

Not at this time. Plus products are specially formulated to be universally compatible with all CNV and Gene Expression CodeSets

Performance of Panel-Plus Gene Sets with pre-designed Panels (Standard Gene Expression and Single Cell Analysis)

Two experiments were performed by adding Panel-Plus genes to the Cancer Reference Panel. Data in each figure below demonstrate that addition of CodeSet-Plus probes does not impact performance of original nCounter Panel probes or Single Cell Panel probes.

Gene Expression Cancer Reference Panel with 30-gene Plus

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100 ng) was hybridized with either nCounter Cancer Reference Panel (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the Original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

Single Cell Gene Expression with Cancer Reference Panel with 30-gene Plus

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100 pg) was converted to cDNA and enriched via a Multiplexed Target Enrichment (MTE) with primers appropriate for downstream hybridization according to the nCounter Single Cell Expression Assay Protocol for Total RNA. The enriched cDNA was hybridized with either nCounter Cancer Reference Panel CodeSet (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

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