nCounter® Low RNA Input Kit

The nCounter Low RNA Input Kit enables the generation of high quality gene expression profiling of up to 800 gene targets from as little as 1 ng of sample. The kit is optimized for use with RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissue as well as crude cell lysates. Additionally, the kit can be utilized in the study of low-expressing genes. The streamlined, user friendly workflow and reliable results enable gene expression studies of small samples or low expressing genes to be completed quickly and efficiently.

High Sensitivity

  • Perform gene expression analysis on up to 800 genes from as little as 1 ng of RNA

Optimized for FFPE

  • Analyze FFPE & other degraded samples from as little as 10 ng of RNA

Simple Workflow

  • Only 30 minutes of hands-on time

Detect Low Expressing Genes

  • The Low RNA Input kit increases nCounter sensitivity & enables detection of low expressing genes

nCounter Gene Expression Assay Compatibility

  • Primer pools are available for all nCounter Gene Expression Panels; Custom designs are available
List of Support Panels
CAE Panel Human Cancer Reference Panel
Human Stem Cell Panel NanoString Custom Panel Codesets*
Human Immunology V2 Mouse Immunology V1
Human Inflammation V2 Mouse Inflammation V2
PanCancer Immune Profiling Panel PanCancer Pathways Panel
PanCancer Progression Panel PanCancer Mouse Immune Profiling Panel
nCounter Human Myeloid Innate Immunity V2 nCounter Mouse Myeloid Innate Immunity V2


Low RNA Input Kit Specifications
Input Amount Purified Fresh / Frozen 1 ng

Cell Lysate 1 ng (~100 cells)
Purified FFPE 10 ng
Number of Samples 48 Reactions
Hands on Time 30 Minutes
Assay Time 2.5 Hours
Compatible Products

Pre-configured NanoString Gene Expression Panels

Custom Panel Support


Storage Conditions
Panel Primer Pools Stable for up to 1 year at -80ºC

Low RNA Input Kits Reagents Store for up to 1 year at -20ºC
NOTE: Low RNA Input Kit reagents and Panel-specific Primer Pools are ordered individually shipped in separate boxes under different shipping conditions.

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Low RNA Input Reagent Kit Low RNA Input Kit 48rx kit for profiling from low sample input amounts. 48 reactions LOW-RNA-48
Panel-Specific Primer Pools for use with Low RNA Input Kit CAE Panel Primer Pool MTE primer pool for Low Input RNA profiling 47 MAQC genes cancer-related human genes. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-CAE-12
Human Cancer Reference Panel Primer Pool MTE primer pool for Low Input RNA profiling 233 cancer-related human genes. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-CR-12
Human Stem Cell Panel Primer Pool MTE primer pool for Low Input RNA profiling 181 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-SC-12
Human Immunology V2 Primer Pool MTE primer pool for Low Input RNA profiling (584 genes) 569 cancer-related human genes + 15 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-HIM2-12
Human Inflammation V2 Primer Pool MTE primer pool for Low Input RNA profiling (249 genes) 243 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-HIN2-12
Mouse Immunology V1 Primer Pool MTE primer pool for Low Input RNA profiling (559 genes) 549 cancer-related human genes + 14 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-MIM1-12
Mouse Inflammation V2 Primer Pool MTE primer pool for Low Input RNA profiling (248 genes) 242 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-MIM2-12
PanCancer Immune Profiling Panel Primer Pool MTE primer pool for Low Input RNA profiling (768 genes) 728 cancer-related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-HIP1-12
PanCancer Pathways Panel Primer Pool MTE primer pool for Low Input RNA profiling (739 genes) 733 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-PATH-12
PanCancer Progression Panel Primer Pool MTE primer pool for Low Input RNA profiling (770 genes) 730 cancer-related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-PROG1-12
PanCancer Mouse Immune Profiling Panel Primer Pool MTE primer pool for Low Input RNA profiling (770 genes) 750 cancer-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-MIP1-12
nCounter Human Myeloid Innate Immunity V2 Panel Primer Pool MTE primer pool for Low Input RNA profiling (770 genes) 730 immune-related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-HMII2-12
nCounter Mouse Myeloid Innate Immunity V2 Panel Primer Pool MTE primer pool for Low Input RNA profiling (754 genes) 734 immune-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet required. 12 reactions PP-MMII2-12
Instrument Consumables for use with Low RNA Input Kit nCounter Master Kit (MAX or FLEX systems) Reagents, cartriges, and consumables necessary for sample processing on nCounter MAX and FLEX systems. 12 reactions NAA-AKIT-012
nCounter SPRINT Cartridge Sample cartridge for nCounter SPRINT system. 12 reactions SPRINT-CAR-1.0
nCounter SPRINT Reagent Pack nCounter SPRINT Reagent Pack containing reagents A, B, C, and hybridization buffer. 192 reactions SPRINT-REAG-KIT
  • Low Input Workflow

    Low Input Workflow

  • Gene Expression Fold Change of Low RNA Input Kit (1 ng w/pre-amplification) vs. NanoString Assay Input (100ng, without pre-

    amplification

For Research Use Only. Not for use in diagnostic procedures.