ACD Partnership

The power of single molecule RNAscope™ visualization combined with high-plex GeoMx® RNA assays.


  • ACD.pngMolecularly guide high-plex spatial analyses on GeoMx DSP with single cell resolution

  • Validated protocol with qualified RNAscope probes
  • Integrated manual and automated workflows
  • Fresh frozen and formalin-fixed paraffin-embedded sample compatible


Technology Overview

  • Visualize mRNA transcript for cytokines, transcription factors, and low expressing targets
  • Choose regions of interest on GeoMx DSP based on critical gene expression for high-plex profiling
  • Quantify 1,800+ gene targets in each region of interest with the GeoMx Cancer Transcriptome Atlas

IM_MK2366_DSP RNAscope Linear Workflow_R5.png


Qualified RNAscope Probe Combinations

  • Target interesting immune biology in fresh frozen (FF) and formalin-fixed paraffin embedded (FFPE) tissues
  • Choose a probe combination from our list or design your own combination
  • Review GeoMx Manual that integrates RNAscope into the GeoMx workflow
  • To learn more or order RNAscope probes listed below visit ACD


Qualified RNAscope Probe Combinations

Target Biology Probe 1 Probe 2 Probe 3
Immune checkpoints PDCD1 (PD-1) CD274 (PD-L1) CD3E
Tumor vs. Stroma PanCK PTPRC (CD45​) CD3E
Cytotoxic T Lymphocytes (Set 1) CD8​A GZMB​ CD3E
Cytotoxic T Lymphocytes (Set 2) CD8​A IFNG​  
T regs​ CD4​ FOXP3​  
Activated T regs​ CD4​ FOXP3​ TIGIT
Immune suppression​ TGFB1 FOXP3​  
Macrophages​ CD68​ CD163 CD3E
M1 Macrophages​ CD68 CXCL10  
M2 Macrophages​​ CD163 CCL22 CD3E


For Research Use Only. Not for use in diagnostic procedures.