NanoString Seminar in Münster
Lunch Seminar: NanoString's Novel Digital Genes & Protein Expression Tools - Host: Jan Bruder
|SPEAKER:||Speaker: Helmut von Keyserling|
Nov 09, 2018
|LOCATION:||MPI for Molecular Biomedicine, Ground Floor Seminar Room, Roentgenstrasse 20, 48149 Münster|
Nanostring utilizes a novel barcode technology for enzyme- and amplification free mRNA, DNA, and protein profiling applications. The single tube and extraction-free workflow requires only 10 minutes of hands-on time to examine multiple pathways with hundreds of genes in up to 96 samples per run. The streamlined workflow eliminates cDNA synthesis and amplification which results in bias-free digital data with less technical variation. The methodology also enables the analysis of any combination of highly multiplexed DNAs, RNAs, and proteins simultaneously using a single detector. We recently refined this so-called 3D Biology approach to tissue sections mounted on glass slides while preserving morphology as well as spatial information. Here we discuss key publications that show the different assays and formats as well as the technical performance of the system.