Investigating the Human Immune Response with Myriad RBM’s TruCulture® System & NanoString® nCounter® Autoimmune Profiling Panel
XTalks Webinar Presentation
|SPEAKER:||Anne Hwang, PhD, Scientist III, Myriad RBM, Inc. & Nathan Elliott, PhD, Scientist II, NanoString Technologies|
Dec 03, 2018
Autoimmunity is used to describe almost 80 unique illnesses that include multiple sclerosis, systemic lupus erythematosus, type 1 diabetes, psoriasis, and rheumatoid arthritis and is also increasingly a complication of immuno-oncology therapy. Research tools that allow for the study and characterization of autoimmune biology and development of gene signatures that predict an autoimmunity flare or response to a given therapy will be valuable assets for the research community. In this webinar, featured speakers will describe a protocol and application for combining Myriad RBM’s TruCulture® system and NanoString® nCounter® gene expression profiling to generate proteomic and transcriptional profiles of blood from healthy donors stimulated with a cytokine (TNFα) or pathogen associated molecule (staphylococcal enterotoxin B, SEB).
Topics covered in the webinar include:
- Applying the TruCulture blood collection and culture system for immune monitoring
- A protocol for isolating RNA from TruCulture samples for downstream analysis using the NanoString nCounter Autoimmune Profiling gene expression panel
- Data demonstrating the reproducibility of the NanoString platform and a correlation between NanoString transcriptional counts and Myriad RBM’s OptiMAP secreted protein measurements
- Combining the TruCulture system with nCounter gene expression profiling enables the study of circulating immune responses using a rapid and easy collection and stimulation culture system followed by subsequent profiling of leukocytes from healthy or diseased cohorts.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.