Masthead

ACD Partnership

Masthead

The power of single molecule RNAscope™ visualization combined with high-plex GeoMx® RNA assays.

  • Molecularly guide high-plex spatial analyses on GeoMx DSP with single cell resolution
  • Validated protocol with qualified RNAscope probes
  • Integrated manual and automated workflows
  • Fresh frozen and formalin-fixed paraffin-embedded sample compatible

Technology Overview

  • Visualize mRNA transcript for cytokines, transcription factors, and low expressing targets
  • Choose regions of interest on GeoMx DSP based on critical gene expression for high-plex profiling
  • Quantify 1,800+ gene targets in each region of interest with the GeoMx Cancer Transcriptome Atlas
IM_MK2366_DSP RNAscope Linear Workflow_R5.png

Qualified RNAscope Probe Combinations

  • Target interesting immune biology in fresh frozen (FF) and formalin-fixed paraffin embedded (FFPE) tissues
  • Choose a probe combination from our list or design your own combination
  • Review GeoMx Manual that integrates RNAscope into the GeoMx workflow
  • To learn more or order RNAscope probes listed below visit ACD

Qualified RNAscope Probe Combinations

TARGET BIOLOGYPROBE 1PROBE 2PROBE 3
Immune checkpointsPDCD1 (PD-1)CD274 (PD-L1)CD3E
Tumor vs. StromaPanCKPTPRC (CD45​)CD3E
Cytotoxic T Lymphocytes (Set 1)CD8​AGZMB​CD3E
Cytotoxic T Lymphocytes (Set 2)CD8​AIFNG​ 
T regs​CD4​FOXP3​ 
Activated T regs​CD4​FOXP3​TIGIT
Immune suppression​TGFB1FOXP3​ 
Macrophages​CD68​CD163CD3E
M1 Macrophages​CD68CXCL10 
M2 Macrophages​​CD163CCL22CD3E

For Research Use Only. Not for use in diagnostic procedures.