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nCounter® Vantage 3D™ Immune Assays

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Quantify protein and gene expression in one experiment with nCounter® Vantage 3D™ Immune Assays. Our immune assays are designed to complement our 770-plex immune-focused mRNA panel for in-depth characterization of up to 800 total targets:

RNA:Protein Immune Cell Profiling

Track RNA and protein expression of key surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status.

We are thrilled to announce that we are a winner for The Scientist Top 10 Innovations 2016 Award

RNA:Protein Immune Cell Signaling

Measure RNA and intracellular protein levels of cytokines, chemoattractants, and transcription factors for the earliest indications of cell-cell communication and immune differentiation.

Protein signals from nCounter® Vantage 3D™ panels correlate strongly (R2>0.75) with gold-standard flow cytometry for robust, reliable detection in cell suspension samples.

Note: All of the above panels can also be run in single analyte experiments and can be mixed and matched with other nCounter Vantage 3D Portfolio products.

Advantages of RNA:Protein assays include:

  • More data from your precious samples: Simultaneous analysis of RNA and protein enables you to see more from a single sample using as few as 20,000 cells (50,000 cells for PBMCs and primary cells).
  • Simple integrated workflow and holistic data output: Integrated measurements simplify your research and eliminate the challenge of comparing data from different technologies with different outputs.
  • Compatible with your samples: Our protocols enable you to run both PBMCs and other primary cells without compromising reproducibility.
  • Easy data management: No bioinformatician required, our Advanced Analysis software includes statistical tests and produces publication-quality figures.
  • Flexible design: Customize through the addition of up to 3 proteins of your choice with Vantage 3D™ Protein Barcoding Service.
Illustration of the nCounter RNA:Protein assay workflow
Illustration of the nCounter RNA:Protein assay workflow.


Investigate the Human Cancer Immunity Cycle

  • Gene Expression
  • Proteins
  • Protein Barcoding
  • Ordering Info

Protein Barcoding Service

Our nCounter Vantage 3D Protein Barcoding Service provides you with the additional flexibility you need, allowing inclusion of up to 3 custom protein probes with the addition of your selected antibodies to our pre-designed panel. Your antibodies will be labeled with unique color-coded molecular barcodes to enable direct quantification of your protein of interest on the nCounter platform through detection of the antibody oligonucleotide conjugates. Custom development of protein assays is also available through the Protein Barcoding Service.

Contact us for more information below.

For a full list of genes and proteins, please download our white paper.

To learn more about nCounter Vantage 3D Portfolio, click here.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.


Request Information | E-mail us: info@nanostring.com | Call us: 1 888 358 6266