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nCounter® Vantage 3D™ Solid Tumor Assays


Profile mutational status and quantify protein and gene expression in one experiment with nCounter® Vantage 3D™ DNA, RNA, and Protein Solid Tumor panels. Our protein panels are designed to complement our 770-plex pathway-focused mRNA panel for in-depth characterization of up to 800 total targets.

The DNA SNV Solid Tumor Panel is designed with content matched to the Solid Tumor RNA:Protein Assays and enable detection of SNV in 25 key solid tumor driver genes. Together, the nCounter Vantage 3D Solid Tumor assay allows you to profile both genotype and phenotype from your precious samples and see more biology.

We are thrilled to announce that we are a winner for The Scientist Top 10 Innovations 2016 Award

DNA SNV Solid Tumor Panel
Monitor 104 actionable and high-frequency SNV and INDELS in FFPE, fresh/frozen tissues, and cells. When combined with our nCounter Vantage 3D RNA:Protein Assays, you can analyze and validate DNA mutations and gene and protein expression changes in your precious samples to uncover the novel solid tumor biology.

RNA:Protein Solid Tumor Assay for Lysate
Profile key cancer pathway targets at both the RNA, total protein, and phospho-protein level, including targets associated with PI3K, MAPK, EGFR, and more in your solid tumor tissue and cell lysates.

RNA:Protein Solid Tumor Assay for FFPE
Our first FFPE-compatible protein panel profiles many of the same key signaling pathways at the RNA, protein, and phospho-protein level as the lysate panel above with a modified protocol for your FFPE tissue.

DNA:Fusion Lung Assay
Combined DNA:RNA analysis brings together matched SNV and Fusion content designed with the lung researcher in mind. Simultaneous analysis ensures none of your precious samples go to waste and you collect the data you need.

Note: All of the above panels can also be run in single analyte experiments and can be mixed and matched with other nCounter Vantage 3D Portfolio products.

Advantages of the Solid Tumor Assays include:

  • More data from your precious samples: Simultaneous analysis of DNA, RNA, and protein enables you to see more from a single sample using as few as 5 ng DNA, 25 ng RNA, and 250 ng protein or 2 FFPE slides.
  • Simple integrated workflow and holistic data output: Integrated measurements simplify your research and eliminate the challenge of comparing data from different technologies with different outputs.
  • Compatible with your samples: Our protocols enable you to run tissue and cell lysates in addition to FFPE with all analytes.
  • Easy data management: No bioinformatician required, our Advanced Analysis software includes statistical tests and produces publication-quality figures with multi-analyte data.
  • Flexible design: nCounter Vantage 3D panels can be mixed and matched to meet your research goals and panel content can be customized to include your targets of interest.

nCounter Vantage 3D Workflow, NanoString Technologies, Inc.
Illustration of the nCounter Vantage 3D workflow. Up to 800 DNA, RNA, and protein targets can be quantified simultaneously with additional data merging capabilities in silico.

Note: SNV detection requires pre-amplification prior to hybridization. Results using the nCounter Vantage 3D panel show reliable detection down to 5% mutant DNA and >95% specificity.

Protein counts using the nCounter Vantage 3D panels correlate strongly (R2>0.9) with gold-standard ELISA or IHC for robust, reliable digital detection.

Capture More Biology from Less Sample

  • SNV
  • Gene Expression
  • Lung Fusions
  • Protein
  • Ordering Info

  • 104 driver mutations from 25 genes
  • Panel includes mutant probes for each SNV and reference probes per gene
  • Only 5 ng gDNA input requirement from FFPE and other sample types
  • Content spans brain, breast, thyroid, pancreatic, lung, ovarian, urothelial, and melanoma

Protein Solid Tumor content is designed to pair with the PanCancer Pathways, which profiles 770 RNA representing 13 canonical pathways, including MAPK, PI3K, and more. SNV analysis is curated to provide additional biological insight and can be merged in silico for complete multi-analyte profiling.

nCounter Vantage 3D RNA panels are also compatible with SNV and protein profiling for complete flexibility with our mix-and-match capabilities for single-lane analysis.




RNA Customization

nCounter Vantage 3D RNA Panels (including fusions) are available with the option to customize with up to 24 user-defined genes. Customizing a panel allows researchers the convenience of an off-the-shelf product along with the flexibility to create a panel that is uniquely your own.

Contact us for more information.


  • Multiplexed gene fusion detection designed with the lung researcher in mind.
  • Detect rare fusions with highly specific probes designed using patented Junction Sequence design technology.
  • Ideal for use with challenging sample types including FFPE tissue.
  • Content is compatible with SNV and protein Solid Tumor analysis for an integrated multi-analyte solution to profile your precious lung tissue FFPE samples.

Multiplex analysis of phospho- and total proteins associated with key solid tumor signaling pathways.

  • Solutions available for both cell/tissue lysate and FFPE
  • Just 250 ng protein or 1 FFPE slide required for analysis of up to 28 targets
  • Protein panels can be customized with the addition of up to three antibodies
  • Panels are compatible with all nCounter Vantage 3D products as well as all Gx panels, including custom CodeSets

Protein Barcoding Service

Our nCounter Vantage 3D Protein Barcoding Service provides you with the additional flexibility you need, allowing inclusion of up to three custom protein probes with the addition of your selected antibodies to our pre-designed panel. Your antibodies will be labeled with unique color-coded molecular barcodes to enable direct quantification of your protein of interest on the nCounter platform through detection of the antibody oligonucleotide conjugates. Full custom development of protein assays is also available through the Protein Barcoding Service.

Contact us for more information.

Ordering Information

Name Format Description Quantity Catalog Number
nCounter Vantage 3D DNA SNV Solid Tumor Panel Code Set Only For use with nCounter Vantage 3D RNA and/or protein or as a stand-alone DNA assay. 12 reactions VDXC-HST-12
nCounter Vantage 3D SNV Qualification Kit Code Set Only Pre-qualification kit containing synthetic oligos to qualify nCounter MAX/FLEX before DNA SNV testing. 12 reactions VDXC-QualK-12
nCounter Vantage 3D Lung Fusion Panel Code Set Only For use with nCounter Vantage 3D DNA and/or protein or as a stand-alone fusion assay. 12 reactions VDXC-HST-12 and VRXC-LGF-12*
nCounter Vantage 3D RNA:Protein Solid Tumor Assay for Lysates Code Set Only Prematched RNA:Protein assay. 12 reactions VRPC-HSTL-12
nCounter Vantage 3D Protein Solid Tumor Panel for Lysates (D) Code Set Only For use as a stand-alone protein assay or with nCounter Vantage 3D DNA 12 reactions VPODC-HSTL-12
nCounter Vantage 3D Protein Solid Tumor Panel for Lysates (R) Code Set Only For use with RNA (+/- DNA) including custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA 12 reactions VPRXC-HSTL-12
nCounter Vantage 3D RNA:Protein Solid Tumor Assay for FFPE Code Set Only Prematched RNA:Protein assay 12 reactions VRPC-HSTF-12
nCounter Vantage 3D Protein Solid Tumor Panel for FFPE (D) Code Set Only For use as a stand-alone protein assay or with nCounter Vantage 3D DNA 12 reactions VPODC-HSTF-12
nCounter Vantage 3D Protein Solid Tumor Panel for FFPE (R) Code Set Only For use with RNA (+/- DNA) including custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA. 12 reactions VPRXC-HSTF-12
nCounter Vantage 3D Protein Barcoding Service Service Customization Service for spike-in to above protein panels or full custom panel development 1 each PBS-1AB
nCounter Vantage 3D Protein Barcoding Service TagSet (D) Tag Set Only Protein detection reagent to support a custom stand-alone protein assay or custom protein for use with nCounter Vantage 3D DNA 12 Reactions PBS-POD-12
nCounter Vantage 3D Protein Barcoding Service TagSet (R) Tag Set Only Protein detection reagent to support custom protein use with RNA (+/-DNA) including custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. 12 Reactions PBS-PXR-12
nCounter Vantage 3D Protein Barcoding Service - Cross Reactivity Testing Service Cross Reactivity Service for spike-in custom protein only 1 each PBS-XTEST
nCounter Master Mix Kit (Max or Flex Systems) Reagents and Cartridges Reagents, cartridges, and consumables necessary for sample processing. 12 reactions NAA-AKIT-012
nCounter SPRINT™ Cartridge Reagents and Cartridges 1 Cartridge, 12 lanes 12 reactions SPRINT-CAR-1.0
nCounter SPRINT™ Reagent Pack Reagents and Cartridges Reagents A,B,C & Hybridization Buffer 192 reactions SPRINT-REAG-KIT

*Both part numbers are required for Vantage 3D Lung Fusion Panel.


To learn more about our nCounter Vantage 3D Portfolio, click here.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.





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