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nCounter® PlexSet™ Reagents

Simple, cost-effective multiplex gene expression analysis for high throughput research applications.

Product Highlights

  • Multiplex up to 24 custom probes across 96 samples to generate 2,304 data points per run
  • “Lyse-and-go” protocol for cells eliminates the need for RNA purification, reverse transcription, and amplification
  • Digital gene expression provides accurate reproducible results
  • No upfront probe optimization required
  • Only 30 minutes of hands-on time; no need for cDNA conversion*, amplification*, or replicates
*Single cell protocol requires amplification

Simple “lyse-and-go” protocol for cell samples

The protocol offers a simple workflow to lyse cells with the nCounter Buffer LH and to directly use them as samples in the experiment to attain specific and sensitivegene expression analysis.

The “lyse-and-go” protocol helps researchers save time and resources. Figure above showcases three graphs demonstrating high concordance between 5,000-20,000 lysed cells and 50-200 ng total purified RNA from Jurkat cells using PlexSet technology.

Product Information

  • Product Specs
  • Support Documents
  • Product Details

Product Specifications

Feature Specifications
Number of Targets Up to 24 targets (provided by users to bioinformatics@nanostring.com ); 4 internal controls already included
Minimum Input Material 50 ng of total purified RNA or 5,000 cells or 1 ng of RNA input with amplification
Sample Type(s) Total purified RNA (cells or tissues), cell lysates, Fresh frozen or FFPE-derived RNA, amplified RNA
Species Any
Time to Results ~30 minutes- hands on time; <24 hours - time to results
Data Analysis nSolver™ Analysis software
Shipping and Storage Conditions -80°C (Hyb buffer kit at 4°C)

To receive a quote, please fill out the Request Information form.

Product Details

Product Description Catalog Number Unit Size
nCounter PlexSet-12 Reagent Pack For custom Gene Expression analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits. PS-012-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-12 For custom Gene Expression analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only—does not include Master Kits. PS-GX-PTK-12 (CSO) 24 reactions
nCounter PlexSet-24 Reagent Pack Kit is set up to perform titration for 12-gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits. PS-024-GX-192S (CSO) 192 reactions
nCounter PlexSet Titration Kit-24 Kit is set up to perform titration for 24 gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only- does not include Master Kits PS-GX-PTK-24 (CSO) 24 reactions
Buffer LH Bottle (50mL) Buffer LH is a lysis buffer optimized to lyse cells and utilize them directly with PlexSet reagents to enable a “lyse-and-go” protocol. One 50 ml bottle is sufficient to analyze samples for 8 plates (4 pack or 768 samples) of PlexSet reagents. PS-GX-BLH 768 reactions

To receive a quote, please fill out the Request Information form.


Request Information | E-mail us: info@nanostring.com | Call us: 1 888 358 6266