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Panel-Plus and CodeSet-Plus
nCounter® Panel-Plus & CodeSet-Plus















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nCounter® Panel-Plus & CodeSet-Plus

Providing a New Level of Flexibility to nCounter Experiments

The nCounter® Panel-Plus & CodeSet-Plus products add a new level of flexibility to nCounter experiments. Researchers can now customize any off-the-shelf panel kit or add genes to any custom CodeSet by utilizing up to 30 unique Reporter Codes exclusively formulated for use in Panel-Plus and CodeSet-Plus products.

Product Highlights

  • Compatible with all Gene Expression and CNV products including Single Cell applications
  • 30 unique Reporter Codes are universally compatible with all custom Panels and CodeSets

Applications

  • Customize a Panel: Add up to 30 of your favorite genes or a collection of specific controls
  • Update a large CodeSet: Utilize results from your experiments and add new genes as you go to keep your CodeSet updated
  • Create specific control sets: Create multiple Plus control sets for use with different sample or cell types
  • Finalize your gene list more quickly: Add more genes later

WORKFLOW: Setting Up Panel-Plus or CodeSet-Plus Reactions

nCounter Panel-Plus and CodeSet-Plus

nCounter® Panel-Plus & CodeSet-Plus Specifications

  • Ordering Info
  • Panel-Plus
  • CodeSet-Plus
  • Performance

Design and Ordering Information

Description Number of Genes No. of Reactions
nCounter Panel-Plus 6 - 10 genes
11 - 20 genes
21 - 30 genes
48 minimum
nCounter CodeSet-Plus 6 - 10 genes
11 - 20 genes
21 - 30 genes
48 minimum


To receive a quote, please fill out the Request Information form.

nCounter Panel-Plus

The Panel-Plus product enables any off-the-shelf panel kit to be customized with up to 30 additional genes of interest. Customizing a panel kit with a Panel-Plus product allows researchers to leverage the bioinformatics knowledge incorporated into every nCounter Panel Kit and provides unprecedented flexibility to tailor a comprehensive list of genes to your exact needs. In addition using a panel kit combined with Panel-Plus will allow you to survey a comprehensive collection of genes for a lower cost per sample when compared to the same gene set being ordered as a custom CodeSet.

For more information, please fill out the Request Information form.

nCounter CodeSet-Plus

The CodeSet-Plus product provides researchers the ability to update custom CodeSets that may be used to analyze large sample sets over many months. Genes of interest that become known from ongoing research, or from a new publication, can now easily be added to a custom CodeSet. This flexibility means that a custom CodeSet sufficient to analyze all the samples in a large study can be purchased with confidence. Purchasing a larger custom CodeSet provides a more cost effective solution versus purchasing multiple smaller custom CodeSets.

For more information, please fill out the Request Information form.

Performance of Panel-Plus Gene Sets with Pre-designed Panels
(Standard Gene Expression and Single Cell Analysis)

Two experiments were performed by adding Panel-Plus genes to the Cancer Reference Panel. Data in each figure below demonstrate that addition of CodeSet-Plus probes does not impact performance of original nCounter Panel probes or Single Cell Panel probes.

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100ng) was hybridized with either nCounter Cancer Reference Panel (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the Original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100pg) was converted to cDNA and enriched via a Multiplexed Target Enrichment (MTE) with primers appropriate for downstream hybridization according to the nCounter Single Cell Expression Assay Protocol for Total RNA. The enriched cDNA was hybridized with either nCounter Cancer Reference Panel CodeSet (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel-Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

View the full data in the Product Data Sheet.


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