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The nCounter Analysis System is a complete, fully automated system for the next generation of digital gene expression analysis. Extraordinarily easy-to-use, it provides everything a lab needs to move from the past to the future of genomics research. Through the use of a unique coding technology, direct counting of individual mRNA molecules is made possible across all levels of biological expression. Requiring only 100ng of total RNA or equivalent amounts of raw cell or blood lysate to study hundreds of genes, the simple automated workflow can be easily incorporated into any existing laboratory.
System Highlights:
- Robust Engineering
- Fully-Automated
- Easy Workflow
- State-of-the-Art User Interface
nCounter Prep Station
The nCounter Prep Station is the automated fluidic handling component of the nCounter Analysis System. It processes samples post-hybridization to prepare them for data collection on the nCounter Digital Analyzer. Prior to placing samples on the Prep Station, samples need to be hybridized according to the nCounter protocol. On the deck of the Prep Station, hybridized samples are purified and subsequently immobilized in the sample cartridge for data collection. All consumable components and reagents required for sample processing on the Prep Station are provided in the nCounter Master Kit, ready to load on the deck of the robot. The Prep Station can process up to 12 samples per run in approximately 2 hours.
nCounter Digital Analyzer
The nCounter Digital Analyzer collects data by taking images of the immobilized flourescent reporters in the sample cartridge with a CCD camera through a microscope objective lens. Images are processed internally and the results are exported as a .csv file. The file can be processed with Excel® or by all commonly used data analysis and visualization packages.
The nCounter Digital Analyzer comes with the nCounter Imaging Oil Applicator, a stand-alone unit used to apply precise amounts of oil to the nCounter cartridge prior to data collection. The application of optical oil is important for achieving high quality images.
