NEW! nCounter SPRINT™ Profiler
For Research Labs
Affordable benchtop device used to analyze differential expression of genes and proteins.
Maximize Sample: Don’t let samples limit your experimental design. Analyze 10s to 100s of targets simultaneously using single-tube multiplexing, and avoid waste by eliminating RNA extraction; process crude cell lysates from as few as 2,500 cells.
Boost Productivity: Enjoy an intuitive workflow that requires only ten minutes of hands-on time from sample to data. Deploy this technology without the need to hire additional staff, and free your team to advance other parts of your research project.
Simplify Data: Don’t waste time waiting for a bioionformatics specialist to review your data. Get results in a simple CSV file that contains direct counts for each target. Import it into your favorite application or use the included nSolver™ Analysis Software for convenient data analysis.
Detect Small Fold Changes: Eliminate cDNA synthesis, amplification*, and library prep so you experience less technical variation in your assay. See real differences in your sample and work faster by reducing the need for experimental replicates.
* Single cell assays require amplification prior to sample processing and data collection.
nCounter® SPRINT™ Profiler Specifications
- Supported Applications
- Compatible Chemistries
|RNA:Protein||Perform multianalyte measurement of both RNA and proteins simultaneously.|
|mRNA||Automated RNA profiling without cDNA synthesis, library prep, or amplification.|
|miRNA||Specific measurements of microRNA transcriptomes from human, mouse, and rat.|
|CNV||Highly accurate and reproducible detection of gene copy number variation.|
|Fusion Genes||Find breakpoint locations and specific junction sequences.|
Compatible Barcode Chemistries
|For Pathway Analysis||For Lab Tests|
|nCounter CodeSets||nCounter Elements|
|Targets per sample||20 - 800||1 - 216|
|Samples per Lane||Up to 2||1|
|Total samples per run||Up to 24||12|
*Assay comes with gene-specific oligos and/or epitope-specific antibodies.
|nCounter SPRINT Cartridge|
|nCounter SPRINT Reagent Pack|
|For Design||For Operation||For Analysis|
|nDesign™ Gateway||nCounter SPRINT Control Center||nCounter SPRINT iPhone® App||nSolver Analysis Software|
|Create custom nCounter CodeSets.||Set up runs and access data from your desktop.||Coming Soon! Monitor your run from anywhere.||Automated QC, normalization, and analysis of your nCounter data.|
Platform Performance Specifications
|Level of multiplexing||Up to 800 targets|
|Recommended amount of starting material||RNA:
- 25 ng > 400 target plex
- 50 ng < 400 target plex
- 150 ng
- 200-2,500 cell equivalent lysates
|Sample types supported||Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene lysed whole blood|
|Reaction volume||Up to 35 μL|
|Limit of detection 0.5fM spike-in control||15 zeptomole spike-in control|
|Fold change sensitivity||> 1.5-fold (if > 5 copies per cell)
> 2-fold (if > 1 copy per cell)
|Spike correlation||R² ≥ 0.95|
|Linear dynamic range||6 x 10⁵ total counts|
|Throughput||12 lanes per 6 hours|
|Power source||100-240 VAC, 50-60 Hz|
|Dimensions||107 x 72 x 82 cm|